A5100446949- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Food composition and properties
- Advanced biosensing and bioanalysis techniques
- Biofuel production and bioconversion
- Biosensors and Analytical Detection
- Polysaccharides Composition and Applications
- Pancreatic function and diabetes
- Phytase and its Applications
- Enzyme Production and Characterization
- Protein Interaction Studies and Fluorescence Analysis
- Protein Hydrolysis and Bioactive Peptides
- Polyamine Metabolism and Applications
- Fungal Biology and Applications
- Metal complexes synthesis and properties
- Medicinal Plants and Bioactive Compounds
- Alcohol Consumption and Health Effects
- Drug Transport and Resistance Mechanisms
- Polysaccharides and Plant Cell Walls
- Proteins in Food Systems
- Endoplasmic Reticulum Stress and Disease
- Fermentation and Sensory Analysis
- Gut microbiota and health
- Fungal and yeast genetics research
- Electrochemical sensors and biosensors
Shandong University of Technology
2017-2025
Zhejiang University of Science and Technology
2012-2025
University of Jinan
2023-2025
Medical Process (France)
2025
Sichuan Agricultural University
2023-2024
Sichuan University of Science and Engineering
2024
Ministry of Agriculture and Rural Affairs
2024
Beijing Proteome Research Center
2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
Jiangnan University
2018-2021
To develop a high throughput colorimetric biosensor for detection of Staphylococcus aureus (SA) based on specific aptamer and catalysis dsDNA-SYBR Green I (SG I) complex. SA was immobilized 96-well plate by hybridization with the capture probe anchored surface through streptavidin-biotin binding. In presence SA, dissociated from probe-aptamer duplex due to stronger interaction between SA. The consequent single-strand could be hybridized three-way junction (TWJ) probe. With SG I, dsDNA-SG...
γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways some plant microbial GLA have been deciphered, current understanding correlation between desaturases synthesis in oleaginous fungi incomplete. In previous work, we found that a large amount oleic (OA) had not converted to linoleic (LA) or Mucor circinelloides CBS 277.49, which may be due inadequate activities delta-12 delta-6 desaturases, thus leading...
Alkaline sphingomyelinase (alk-SMase) hydrolyses sphingomyelin (SM) to ceramide in the gut. To evaluate physiological importance of enzyme, we generated alk-SMase knockout (KO) mice by Cre-recombinase-Locus X-over P1(Cre-LoxP) system and studied SM digestion. Both wild-type (WT) KO were fed 3H-palmitic acid labeled together with milk gavage. The lipids intestinal content, tissues, serum, liver analyzed TLC. In mice, nondigested 3H-SM content increased 6-fold formation 3H-ceramide decreased...
Increasing energy demands and health-related concerns worldwide have motivated researchers to adopt diverse strategies improve medium-chain fatty acid (MCFA) biosynthesis for use in the functional food aviation industries. The abundance of naturally produced MCFAs from botanical sources (i.e., coconut fruit/seeds palm tree) has been observed be insufficient compared with various microorganisms used cope industrial demands. Mucor circinelloides is one many promising microorganisms; it...
Summary The effects of reduced glutathione (GSH) on dough rheology, water state and distribution, gluten conformation, protein molecular weight distribution were investigated. Addition GSH (0.02−0.04%) resulted in a more viscous less elastic with decreased G ′ increased tanδ values, which suggested cross‐links network weakened structure. proteins was by the GSH‐induced cleavage intermolecular disulphide bonds. Fourier transform infrared spectroscopy showed high fraction β‐sheet formation at...
Abstract Febrifugine is a kind of quinazolinone compound with high biological activity from Chinese herb called Chang Shan ( Dichroa febrifuga ). and its derivatives possess extensive activities, some which exhibited anti‐tumor activities as FAK inhibitors. However, they are not very effective at inhibiting tumor metastasis, perhaps because tumors gain energy through compensatory activation other signaling pathways that promote cell migration invasion. Therefore, seventeen novel febrifugine...
This study aimed to compare the effects of various selenium (Se) sources (2 mg/kg) on performance, quality, and antioxidant capacity laying hens as well Se content in their eggs blood. We selected 720 34-wk-old Lohmann pink-shell were randomly assigned into 6 groups fed a basal diet (control) or supplemented with (Se-enriched yeast, SY-A, SY-C, SY-N; selenomethionine SM, nano-Se SN) for 16 wk. There 10 replicates 120 per group. Dietary supplementation increased egg production rate all hens....
Microbial lipases could be used to hydrolyze or recombine fats and oils, had great applications in food processing, bioenergy chemical industry. Mucor circinelloides was an important gamma-linolenic acid producing strain, its genome predicted contain a large number of genes encoding lipases, the key enzymes lipid metabolism. In present study, potential lipase WJ_23 from WJ11 cloned for first time heterologously expressed purified homogeneity Pichia pastoris. By SDS-PAGE analysis, molecular...
Abstract Background Gut microbial compositional and functional variation can affect health production performance of farm animals. Analysing metabolites in biological samples provides information on the basic mechanisms that well-being traits However, extent to which host breeds gut microbiome serum metabolome meat rabbits is still unknown. In this study, differences phylogenetic composition capacities microbiota two commercial rabbit Elco Ira were determined by 16S rRNA gene metagenomic...
Abstract In our previous work, we reported a novel approach for increasing lipid production in an oleaginous fungus Mucor circinelloides by overexpression of mitochondrial malate transporter protein. This plays vital role fatty acid biosynthesis during and citrate transport systems fungi. this study, the controlling metabolic supplementation strategy was used to improve protein M. strain coded as Mc-MT-2. The effects different intermediates on batch fermentation Mc-MT-2 were investigated....
The aim of this study was to evaluate the contribution mitochondrial DNA (mtDNA) mutations in oxidative phosphorylation (OXPHOS) deficiency. complete genomes 41 families with OXPHOS deficiency were screened for mutations. Mitochondrial functional analysis then performed primary and cybrid cells containing candidate identified during screening. A novel NADH dehydrogenase 5 (ND5) m.12955A > G mutation a patient exercise intolerance developmental delay. biochemical revealed deficiencies...
Concerns about global warming, fossil-fuel depletion, food security, and human health have promoted metabolic engineers to develop tools/strategies overproduce microbial functional oils directly from renewable resources. Medium-chain fatty acids (MCFAs, C8–C12) been shown be important sources due their diverse biotechnological importance, providing benefits ranging lipids uses in bio-fuel production. However, oleaginous microbes do not carry native pathways for the production of MCFAs,...