A5040494365- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiac Ischemia and Reperfusion
- Nitric Oxide and Endothelin Effects
- Anesthesia and Neurotoxicity Research
- Anesthesia and Sedative Agents
- Neuroscience and Neuropharmacology Research
- Hemodynamic Monitoring and Therapy
- Neuroscience and Neural Engineering
- Pain Mechanisms and Treatments
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Sepsis Diagnosis and Treatment
- Airway Management and Intubation Techniques
- Anesthesia and Pain Management
- Pelvic and Acetabular Injuries
- Musculoskeletal pain and rehabilitation
- Cardiac, Anesthesia and Surgical Outcomes
- Restless Legs Syndrome Research
- Eicosanoids and Hypertension Pharmacology
- Renal function and acid-base balance
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ion Transport and Channel Regulation
- Optical Imaging and Spectroscopy Techniques
- Potassium and Related Disorders
Aichi Medical University
2011-2022
Aichi Medical University Hospital
2015-2018
University of Toyama
1993-2011
Wakayama Medical University
2008-2010
Nagoya University
2010
Marymount University
2009
Kagawa University Hospital
2008
Saitama Medical University
2008
Japanese Red Cross Society Wakayama Medical Center
2008
Tohoku Medical and Pharmaceutical University
1995-2006
Abstract Pericytes are believed to originate from either mesenchymal or neural crest cells. It has recently been reported that pericytes play important roles in the central nervous system (CNS) by regulating blood-brain barrier homeostasis and blood flow at capillary level. However, origin of CNS microvascular mechanism their recruitment remain unknown. Here, we show a new source cerebrovascular during neurogenesis. In embryonic day 10.5 mouse embryos, CD31 + F4/80 hematopoietic lineage...
Two new quinochalcone C-glycosides, hydroxysafflor yellow A (1a) and tinctormine (2a), were isolated from Carthamus tinctorius L. (Compositae) together with carthamin, safflor B safflomin C. The structures of 1a 2a have been determined by spectroscopic methods including heteronuclear multiple-bond multiple-quantum coherence linked scan FAB-MS. latter compound (2a) was demonstrated to potent Ca2+ antagonistic action.
Kynurenine is a potential contributor to hypotension in animal and human sepsis. The present study was designed examine whether the voltage-dependent K(+) channels encoded by KCNQ gene family (Kv7 channels) mediate vasodilator effects of kynurenine modulation these ameliorates caused this compound. Rat aortas mesenteric arteries or omental without endothelium were used. Some rings incubated with selective Kv7 channel inhibitor linopirdine (10 μM). l-Kynurenine μM-1 mM) induced...
The use of glucocorticoids for treatment sepsis has waxed and waned during the past several decades, recent randomized controlled trials have evoked a reassessment this therapy. Most glucocorticoid actions are mediated by its specific intracellular receptors (GRs). Thus we initially evaluated whether high-dose corticosteroid therapy can regulate guinea pig pulmonary expression GRs: active receptor, GRα, dominant negative GRβ. Sepsis induction LPS injection (300 μg/kg ip) decreased mRNA...
Supraventricular tachyarrhythmias, including atrial fibrillation, are occasionally observed in patients suffering from sepsis. Modulation of cardiac ion channel function and expression by sepsis may have a role the genesis tachyarrhythmias.Sepsis was induced LPS (i.p.; 300 µg·kg(-1) ) guinea pigs. Membrane potentials ionic currents were measured myocytes isolated pigs 10 h after LPS, using whole cell patch-clamp methods.In cells LPS-treated animals, action potential duration (APD)...
Smooth muscle cells from the rabbit esophageal muscularis mucosae were studied for presence of ATP-sensitive K+ channel (KATP) and its inhibition by carbachol. Lemakalim (10 microM), a synthetic opener, increased whole cell currents -174 +/- 15 pA with 0.1 mM intracellular ATP concentration ([ATP]i) -70 11 5 [ATP]i. Glibenclamide microM) completely abolished lemakalim-induced currents. These therefore denoted as KATP. Carbachol suppressed KATP 74 4% 10 ethylene glycol-bis(beta-aminoethyl...
Neuropathic pain is the most difficult to manage in clinic, and sleep problems are common among patients with chronic including neuropathic pain. In present study, we tried visualize intensity of by assessing neuronal activity investigated disturbance under a pain-like state mice using functional magnetic resonance imaging (fMRI) electroencephalogram (EEG)/electromyogram (EMG), respectively. Furthermore, effect gabapentin (GBP) on these phenomena. model pain, sciatic nerve ligation caused...
We studied the effects of sevoflurane on contraction and membrane potentials in isolated canine ventricular muscle strips. Sevoflurane (>0.5%) depressed electrically induced a dose-dependent manner. Moreover, did not show stimulation frequency-dependent depression as shown with local anesthetics. The inhibitory effect was more pronounced high-K+ Tyrode's solution than normal solution, suggesting that may inhibit transmembrane Ca2+ influx. In contrast, isoflurane halothane were equally...
Sevoflurane can induce prolongation of the cardiac QT interval by inhibiting repolarization phase action potential. This may occur as a result inhibition human ether-a-go-go related gene (HERG) channel. To clarify mechanisms anesthetics on HERG channels, we monitored electrocardiogram and measured intervals in guinea pig presence sevoflurane propofol. (1%-4%) prolonged QTc dose-dependently (7.5%-21.2%), but propofol did not affect it. Furthermore, channels were expressed Xenopus oocytes...
The present study was designed to examine roles of the phosphatidylinositol 3-kinase-Akt pathway and reduced nicotinamide-adenine dinucleotide phosphate oxidases in ATP-sensitive K(+) channel function via superoxide produced by high glucose human artery. We evaluated activity pathway, as well oxidases, intracellular levels omental artery without endothelium. Levels p85-alpha subunit oxidase subunits, including p47phox, p22phox, Rac-1, increased membrane fraction from arteries treated with...
BACKGROUND: Chronic pain is a major problem because it can result in not only reduction activities of daily living and quality life but also requires initiation social assistance. Seeking to eliminate itself would appear be too narrow an objective, addition often being unachievable; therefore, multifaceted, comprehensive approach with multiple objectives needed. OBJECTIVE: To describe the effects program (the ‘Chronic Pain Class’) offering cognitive behavioural therapy small groups...
In Brief Previously, we reported that propofol hyperpolarizes vascular smooth muscle (VSM) cells of small arteries and veins. The current study was designed to determine whether propofol-mediated hyperpolarization is the result specific effects on potassium channels known exist in VSM steps intracellular nitric oxide (NO), cyclic guanosine monophosphate (cGMP), adenosine (cAMP) second messenger pathways. transmembrane potentials (Em) were measured situ sympathetically denervated, mesenteric...
The effects of sevoflurane was examined on the contractile response, membrane potential, ionic currents, and intracellular cyclic adenosine monophosphate (cAMP) in canine single ventricular myocytes.Contraction measured by a video-edge detector, currents were recorded using whole-cell voltage clamp technique. Intracellular cAMP with radioimmunoassay method.Sevoflurane (1.0-4.0%) decreased contraction cells dose-dependent reversible manner. Also, action potential plateau shortened duration...
We studied the role of tyrosine kinase in regulation Ca2+ entry single smooth muscle cells rabbit colonic muscularis mucosae using whole cell patch-clamp technique. Step depolarization to +10 mV from a holding potential -60 produced inward currents that were abolished by 1 microM nifedipine, consistent with activation L-type channels. The inhibitors, genistein and tyrphostin B42, dose dependently inhibited these currents. inactive analogue tyrphostins, A1, did not affect at concentrations up...
It is not known whether thromboxane A2 impairs adenosine triphosphate (ATP)-sensitive K channel function via increased production of superoxide in blood vessels and propofol as a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor restores this modification.Rat aortas without endothelium were used for isometric force recording, measurements membrane potential, Western immunoblotting. Vasorelaxation to an ATP-sensitive opener levcromakalim was obtained during contraction...
Summary: We studied the subcellular mechanisms responsible for negative inotropic effects of two Ic drugs flecainide and pilsicainide. Aequorin luminescence (Ca2+i) isometric tension were recorded simultaneously in isolated trabeculae from dog ventricle. In myocytes same ventricle, slow inward current (ICa) was recorded. Both pilsicainide decreased peak Ca2+i, tension, ICa concentration dependently. Each effect with more marked than that pilsicainide; however, Ca2+i paralleled each other...
The whole cell patch-clamp technique was used to record a transient outward K+ current (ITO) from single smooth muscle cells isolated opossum esophageal circular muscle. threshold for its activation -50 mV holding potentials negative -70 mV. peaked within 10 ms and decayed completely in 200 between test depolarization of -40 -10 ITO recorded at room temperature the presence 5 mM internal ethylene glycol-bis(beta-amino-ethyl ether)-N,N,N',N'-tetraacetic acid. Both inactivation kinetics were...
The root of Solanum sisymbriifolium. Lam (Solanceae), a perennial herb, has been used as traditional medicine possessing diuretic and antihypertensive properties in Paraguay. It was reported that extracts from this plant exerted hypotensive effects rats. Nuatigenosido isolated the extract one prospective active compounds. We have investigated its pharmacological actions. current study demonstrated following results: (1) at 100 µg/kg 1 mg/kg i.v. lowered blood pressure rats, (2) nuatigenosido...