A5103069604- Glioma Diagnosis and Treatment
- interferon and immune responses
- Cancer Mechanisms and Therapy
- Syphilis Diagnosis and Treatment
- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- Reproductive tract infections research
- Neuroblastoma Research and Treatments
- Chromatin Remodeling and Cancer
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- Neonatal Respiratory Health Research
- Photoreceptor and optogenetics research
- Mesenchymal stem cell research
- Single-cell and spatial transcriptomics
- Microtubule and mitosis dynamics
- Neuroscience and Neural Engineering
- Neurogenesis and neuroplasticity mechanisms
- Mathematical Biology Tumor Growth
- Immune cells in cancer
- Computational Drug Discovery Methods
- Neuroscience and Music Perception
- Body Image and Dysmorphia Studies
- Microbial Inactivation Methods
Second Affiliated Hospital of Shantou University Medical College
2015-2024
Shantou University
2015-2024
Key Laboratory of Guangdong Province
2024
Third Affiliated Hospital of Guangzhou Medical University
2023-2024
Guangzhou Medical University
2023-2024
Yale University
2015-2023
Guangdong Province Women and Children Hospital
2021
Amgen (United States)
2021
University of Connecticut
2012-2016
Institute for Systems Biology
2015
Cell death is prevalent throughout life; however, the coordinated interactions and roles of phagocytes during corpse removal in live brain are poorly understood. We developed photochemical viral methodologies to induce single cells combined this with intravital optical imaging. This approach allowed us track multicellular phagocytic precise spatiotemporal resolution. Astrocytes microglia engaged dying neurons an orchestrated synchronized fashion. Each glial cell played specialized roles:...
Abstract irGSEA is an R package designed to assess the outcomes of various gene set scoring methods when applied single-cell RNA sequencing data. This incorporates six distinct that rely on expression ranks genes, emphasizing relative levels over absolute values. The implemented include AUCell, UCell, singscore, ssGSEA, JASMINE and Viper. Previous studies have demonstrated robustness these variations in dataset size composition, generating enrichment scores based solely individual cells. By...
The ability to induce targeted mutations in somatic cells developing organisms and then track the fates of those is a powerful approach both for studying neural development modeling human disease. CRISPR/Cas9 system allows such mutagenesis, we therefore tested it combination with piggyBac transposase lineage labeling neocortical progenitors genes linked neurodevelopmental disruptions tumor formation. We show that sgRNAs designed target PTEN successfully decreased expression, led neuronal...
A major bottleneck limiting understanding of mechanisms and consequences cell death in complex organisms is the inability to induce visualize this process with spatial temporal precision living animals. Here we report a technique termed two-photon chemical apoptotic targeted ablation (2Phatal) that uses focal illumination femtosecond-pulsed laser bleach nucleic acid-binding dye causing dose-dependent apoptosis individual cells without collateral damage. Using 2Phatal, achieve precise...
ArcLight, a genetically encoded fluorescent protein voltage probe with large ΔF/ΔV, is fusion between the sensing domain of Ciona instestinalis sensitive phosphatase and super ecliptic pHluorin carrying single mutation (A227D in protein). Without this produces only very small change fluorescence response to deflections (∼ 1%). The signal afforded by allows optical detection action potentials sub-threshold electrical events single-trials vitro vivo. However, it unclear how such modulation its...
In utero RNAi of the dyslexia-associated gene Kiaa0319 in rats (KIA-) degrades cortical responses to speech sounds and increases trial-by-trial variability onset latency. We tested hypothesis that KIA- would be impaired at sound discrimination. needed twice as much training quiet conditions perform control levels remained several tasks. Focused using truncated was able normalize discrimination background noise conditions. Training also normalized neural temporal phase locking. Cortical...
Human umbilical cord matrix-derived stem cells (uMSCs), owing to their cellular and procurement advantages, are in clinical trials treat type 1 (T1D) 2 diabetes (T2D). Yet the therapeutic basis remains be fully understood. The immunomodulatory property of uMSCs may explain use treating T1D, however mere immune modulation not sufficient support T2D. We thus tested whether could exert direct trophic effects on β-cells. Infusion into chemically induced diabetic rats prevented hyperglycemic...
Dyslexia is the most common developmental language disorder and marked by deficits in reading phonological awareness. One theory of dyslexia suggests that awareness deficit due to abnormal auditory processing speech sounds. Variants DCDC2 several other neural migration genes are associated with may contribute deficits. In current study, we tested hypothesis RNAi suppression Dcdc2 rats causes cortical responses sound impaired discrimination. were subjected utero RNA interference targeting...
The etiology of central nervous system (CNS) tumor heterogeneity is unclear. To clarify this issue, a novel animal model was developed glioma and atypical teratoid/rhabdoid-like (ATRT) produced in rats by nonviral cellular transgenesis initiated utero. This affords the opportunity for directed oncogene expression, clonal labeling, addition tumor-modifying transgenes. By directing HRasV12 AKT transgene expression different cell populations with promoters that are active ubiquitously (CAG...
In utero electroporation (IUE) is an effective transfection method for delivering plasmid DNA into neural progenitor cells and neurons of mammalian neocortex in vivo. Although IUE at multiple plasmids populations cells, unfortunately delivered remain largely episomal often get inactivated or lost after cell division. This results a form “birthdate” labeling which only the types that do not undergo second division continue to express transfected plasmids. limits application with standard...
The heterogeneity of stem cells is a significant factor inhibiting their clinical application, as different cell subpopulations may exhibit substantial differences in biological functions. We performed single-cell sequencing on HUMSCs from three donors gestational ages (22 + 5 weeks, 28 39 weeks). also compared the data with BMSCs two public databases. content CD146+Nestin+ MSCs preterm 5W: 30.2%, 28W: 25.8%) was higher than that full-term (39W: 0.5%) and (BMSC1: 0, BMSC2: 0.9%). Cell cycle...
Syphilis, a sexually transmitted disease, is commonly referred to as the "great imitator" because of its wide-ranging clinical presentations. Recently, we noticed that patients with neurosyphilis (NS) seemed be more susceptible complications diabetes mellitus (DM). This an interesting phenomenon, but it also puzzles clinicians scant knowledge about this situation.A case-control study was conducted explore association between NS and DM. Clinical data prevalence DM among patients, syphilis not...
Optogenetic manipulation with single-cell resolution can be achieved by two-photon excitation. However, this frequently requires relatively high laser powers. Here, we developed a novel strategy that improve the efficiency of current stimulation technologies positioning fluorescent proteins or small molecules cross-sections in vicinity opsins. This generates highly localized source endogenous single-photon illumination tailored to match optimal opsin absorbance. Through neuronal and vascular...
ABSTRACT Background: Neurosyphilis (NS) may present with neuropsychiatric disorders characterized by cognitive impairment, personality disorders, and confusion, among others. Very few studies have focused on secondary to NS in elderly people. Method: A retrospective chart review was performed characterize the psychiatric findings, clinical signs symptoms, laboratory brain magnetic resonance imaging results of ten inpatients NS. Results: In these patients, most common presenting symptoms...
We developed a new Boson chemiluminescence immunoassay (CIA) and evaluated its application with cross-sectional analyses. Our results indicated that the CIA demonstrated strong discriminatory power in diagnosing syphilis it can be used as first-line screening test for serodiagnosis using European Centre Disease Prevention Control algorithm or confirmatory when combined patient's clinical history.
Abstract Background: Despite the progress in perinatal-neonatal medicine, complications of extremely preterm infants continue to constitute major adverse outcomes neonatal intensive care unit. Human umbilical cord Wharton’s Jelly-derived mesenchymal stem cells (HUMSCs) may offer new hope for treatment intractable disorders. This study will explore functional differences HUMSCs between and term infants. Methods: UMSCs from 5 infants(weeks gestation: 22 +5 w,24 +4 w,25 +3 w,26 w,28 w) 2...
Multispecific antibodies, often composed of three to five polypeptide chains, have become increasingly relevant in the development biotherapeutics. These molecules mechanisms action that include redirecting T cells tumors and blocking multiple pathogenic mediators simultaneously. One major challenges for asymmetric multispecific antibodies is generating a high proportion correctly paired antibody during production. To understand causes effects chain mispairing impurities difficult express...
Abstract Optogenetic manipulation with single-cell resolution can be achieved by two-photon excitation; however, this frequently requires relatively high laser powers or holographic illumination. Here we developed a practical strategy to improve the efficiency of stimulation positioning fluorescent proteins small molecules cross-sections in vicinity opsins. This generates highly localized source endogenous single-photon illumination that tailored match optimal opsin absorbance. Through...
Central nervous system tumors are marked with high intra- and inter-tumoral heterogeneity. For instance, an integrative large scale gene expression study performed in 2010 revealed 4 subtypes glioblastoma multiforme (GBM) differential responses to treatment prognosis [1]. A more comprehensive GBM classification based on combination of epigenetics, copy number variation, genetic mutation analysis has led identification as many 6 subgroups [2]. Two, not mutually exclusive, general models...