A5010326726- Reproductive Biology and Fertility
- Ovarian function and disorders
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Ovarian cancer diagnosis and treatment
- Chemotherapy-induced organ toxicity mitigation
- Adipose Tissue and Metabolism
- Regulation of Appetite and Obesity
- Endometriosis Research and Treatment
- Rabbits: Nutrition, Reproduction, Health
- Sirtuins and Resveratrol in Medicine
- Dietary Effects on Health
- Pluripotent Stem Cells Research
- FOXO transcription factor regulation
- Circadian rhythm and melatonin
- Bee Products Chemical Analysis
- Plant tissue culture and regeneration
- Growth Hormone and Insulin-like Growth Factors
- Reproductive Health and Technologies
- Renal and related cancers
- Seed Germination and Physiology
- Phytoestrogen effects and research
- Animal Genetics and Reproduction
- Assisted Reproductive Technology and Twin Pregnancy
Universidade Federal do Vale do São Francisco
2014-2023
Universidade do Vale do Sapucaí
2022
Universidade São Francisco
2015-2019
We investigated the effects of insulin-like growth factor 1 (IGF-1) on morphology and follicular activation ovine preantral follicles cultured in situ whether phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway is involved IGF-1 action sheep ovary. Ovine ovarian fragments were fixed for histological terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) analyses (fresh control) or supplemented alpha-minimum essential medium (α-MEM+; α-MEM+ with (1, 10, 50, 100...
The aim of this study was to evaluate the effects kaempferol on morphology, follicular activation, growth, and DNA fragmentation ovine preantral follicles cultured in situ, a phosphatidylinositol 3 kinase (PI3K) inhibitor expression phosphorylated protein B (pAKT) after culture. Ovine ovarian fragments were fixed for histological terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling (TUNEL) analyses (fresh control) or α‐MEM+ alone (control) with different concentrations (0.1,...
We aimed to study the effects of resveratrol on morphology, DNA fragmentation, follicular activation and cell proliferation after in vitro culture ovine ovarian tissue, verify if PI3K pathway is involved action sheep ovary. Ovaries were collected divided into fragments. One fragment was fixed for histology (fresh control). The remaining fragments cultured 7 days control medium (α-MEM+ ) alone or with (2, 10 30 µM). After culture, tissue destined morphological analysis. TUNEL proliferating...
Summary The present study aimed to investigate the effect of quercetin as an alternative antioxidant cysteamine on in vitro maturation. Oocytes were collected from goat ovaries, destined for maturation and distributed into three groups: CIS group, oocytes immersed MIV base medium; Groups Q4 Q8, medium adding 4 μM or 8 quercetin, respectively, cultured 24 h at 38.5°C with 5% CO 2 . groups presented same percentage expanded cumulus cells, but per cent Q8 group was significantly lower than that...
Summary The worldwide consumption of red wine, nuts and grapes has resulted in increased human exposure to resveratrol, which could affect reproductive function. However, the effect resveratrol on vitro culture early-stage ovarian follicles never been investigated. aims present study were evaluate sheep secondary follicle morphology, growth, DNA fragmentation, intracellular levels glutathione (GSH) active mitochondria. Secondary isolated from ovaries cultured for 18 days supplemented α -MEM...
This study evaluated the effects of kit ligand (KL) on morphology and development ovine preantral follicles (fresh control) after 7 days in vitro culture α-Minimal Essential Medium (α-MEM; control medium) or presence KL (1, 10, 50, 100 200 ng/ml). There was an increase percentage primary at concentration ng/ml KL, compared with fresh control, medium (α-MEM) other concentrations. Follicle diameter significantly higher than only concentrations 50 KL. In conclusion, promoted transition from...
This study aimed to characterize the hydroethanolic extract of red propolis (HERP) and nanoparticles containing HERP for using as an additive in culture medium isolated ovine preantral follicles. was characterized by high-performance liquid chromatography (HPLC) determination flavonoid content, mean particle diameter, polydispersity index (PI) encapsulation efficiency (EE). The effect (10 20 ηg/ml-HERP10 HERP20 groups) (NP10 NP20 on secondary follicles cultured vitro 12 days observed...
Summary The aim of this study was to investigate the effect ovarian tissue transportation conditions (medium and period time) on morphology, apoptosis development ovine preantral follicles cultured in vitro . Each pair cut into nine slices, with one fragment being fixed immediately (fresh control). remaining fragments were placed individually cryotubes containing conservation medium (minimal essential (MEM) without supplementation or MEM + – supplementation) stored at 35ºC for 6 12 h...
Abstract This study analysed the effect of growth differentiation factor‐9 (GDF‐9) on in vitro culture isolated ovine secondary follicles. The follicles were cultured α‐MEM supplemented with BSA, insulin, glutamine, hypoxanthine, transferrin, selenium, ascorbic acid and FSH (α‐MEM + —control medium) or 1, 10, 50 100 ng/ml GDF‐9. Next, oocytes destined to maturation (IVM). After 12 days culture, there no differences regarding percentage normal follicles, antrum formation follicle diameter...
This study evaluated the effects of leptin on primordial follicle survival and activation after in vitro culture ovine ovarian tissue if acts through phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) pathway. Ovarian fragments were fixed for histology (fresh control) or cultured 7 days control medium (α-MEM+) alone supplemented with (1, 5, 10, 25 50 ng/ml). Follicle morphology, apoptosis analyzed. Next, that showed best results absence presence PI3K inhibitor (LY294002),...
This study demonstrated the effect of Morus nigra leaf extract during ovine ovarian tissue transportation on survival and apoptosis preantral follicles in vitro. High Performance Liquid Chromatography (HPLC) was used to determine fingerprint chromatogram crude ethanolic extract. Four pairs ovaries from four sheep were collected. The cortex fragmented one fragment fixed 10% buffered formaldehyde processed for histological TUNEL analysis (fresh control). other fragments placed Minimal...