A5100781841- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Tea Polyphenols and Effects
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Soybean genetics and cultivation
- Plant Parasitism and Resistance
- Photosynthetic Processes and Mechanisms
- Coffee research and impacts
- Plant and animal studies
- Fermentation and Sensory Analysis
- Legume Nitrogen Fixing Symbiosis
- Microbial Natural Products and Biosynthesis
- Plant tissue culture and regeneration
- Phytochemicals and Antioxidant Activities
- Plant Pathogens and Fungal Diseases
- Aluminum toxicity and tolerance in plants and animals
- Plant Surface Properties and Treatments
- Microbial Metabolism and Applications
- Plant Micronutrient Interactions and Effects
- Tannin, Tannase and Anticancer Activities
- Sirtuins and Resveratrol in Medicine
- Light effects on plants
- Electric and Hybrid Vehicle Technologies
Anhui Agricultural University
2017-2025
Shanxi Agricultural University
2025
Huazhong Agricultural University
2015-2024
Beijing Jiaotong University
2024
China Automotive Engineering Research Institute
2024
West Virginia State University
2020
Nanjing Agricultural University
2017
Significance A high-quality genome assembly of Camellia sinensis var. facilitates genomic, transcriptomic, and metabolomic analyses the quality traits that make tea one world’s most-consumed beverages. The specific gene family members critical for biosynthesis key metabolites, monomeric galloylated catechins theanine, are indicated found to have evolved specifically these functions in plant lineage. Two whole-genome duplications, evolution two identified dated, but shown account less...
Tea plant is an important economic crop, which used to produce the world's oldest and most widely consumed tea beverages. Here, we present a high-quality reference genome assembly of (Camellia sinensis var. sinensis) consisting 15 pseudo-chromosomes. LTR retrotransposons (LTR-RTs) account for 70.38% genome, evidence that LTR-RTs play critical roles in size expansion transcriptional diversification genes through preferential insertion promoter regions introns. Genes, particularly those coding...
Flower color is the main character throughout plant kingdom. Though substantial information exists regarding structural and regulatory genes involved in anthocyanin flavonol biosynthesis, little known that what make a diverse white vs. red flower natural species. Here, contents of pigments seven species from varied phylogenetic location plants with flowers were determined. Flavonols could be detected flowers, but anthocyanins almost undetectable cultivar. Comparisons expression patterns gene...
SUMMARY Tea ( Camellia sinensis ) is concocted from tea plant shoot tips that produce catechins, caffeine, theanine, and terpenoids, which collectively determine the rich flavors health benefits of infusion. However, little known about integrated regulation tip development characteristic secondary metabolite biosynthesis in plants. Here, we demonstrate MYB transcription factors (TFs) play key yet diverse roles regulating leaf stem development, biosynthesis, environmental stress responses By...
Summary Tea trichomes synthesize numerous specialized metabolites to protect plants from environmental stresses and contribute tea flavours, but little is known about the regulation of trichome development. Here, we showed that CsMYB1 involved in formation galloylated cis ‐catechins biosynthesis plants. The variations expression levels are closely correlated with indexes contents plant populations. Genome resequencing may be selected modern cultivars, since a 192‐bp insertion promoter was...
Summary Linalool not only is one of characteristic flavour volatiles tea, contributing to floral aroma, but also a kind defensive compounds, playing essential roles in resistance against biotic/abiotic stresses. Although the linalool synthases have been identified, much unknown about regulation mechanism tea plants. We identified two pairs MYB paralogs as biosynthesis activators, which pair (CsMYB148/CsMYB193) specifically expressed flowers, and another (CsMYB68/CsMYB147) highly leaves,...
Summary The MYB ‐ basic helix–loop–helix ( bHLH )‐ WD 40 complexes regulating anthocyanin and proanthocyanidin PA ) biosynthesis in plants are not fully understood. Here Medicago truncatula Mt TT 8 was characterized as a central component of these ternary that control biosynthesis. Mttt8 mutant seeds have transparent testa phenotype with reduced s anthocyanins. restores productions Arabidopsis tt8 mutant. Ectopic expression anthocyanins mttt8 plant hairy roots further enhances both wild‐type...
Summary The multidrug and toxin extrusion ( MATE ) transporter family comprises 70 members in the Medicago truncatula genome, they play seemingly important, yet mostly uncharacterized, physiological functions. Here, we employed bioinformatics molecular genetics to identify characterize transporters involved citric acid export, Al 3+ tolerance Fe translocation. Mt 69 is a induced by Fe‐deficiency. Overexpression of hairy roots altered homeostasis hormone levels under Fe‐deficient or...
SUMMARY Anthocyanin and catechin production in tea ( Camellia sinensis ) leaves can positively affect quality; however, their regulatory mechanisms are not fully understood. Here we report that, while the CsMYB75‐ or CsMYB86‐directed MYB–bHLH–WD40 (MBW) complexes differentially activate anthocyanin biosynthesis leaves, respectively, CsMYBL2a CsMYBL2b homologs negatively modified light‐ temperature‐induced both Arabidopsis plants. The MBW activated synthesis genes downstream repressor ....
Summary Mt PAR is a proanthocyanidin ( PA ) biosynthesis regulator; the mechanism underlying its promotion of not fully understood. Here, we showed that promotes production by direct repression isoflavones, major flavonoids in legume, and redirecting immediate precursors, such as anthocyanidins, flux into pathway. Ectopic expression repressed isoflavonoid directly binding suppressing isoflavone biosynthetic genes synthase IFS ). Meanwhile, up‐regulated ‐specific decreased anthocyanin levels...
Abstract It has been reported that lipid biosynthesis in plant host root cells plays critical roles legume‐fungal or ‐rhizobial symbioses, but little is known about its regulatory mechanism legume–rhizobia interaction. Soybean WRINKLED1 ( WRI1 ) a and b , with their alternative splicing (AS) products a’ b’ are highly expressed developing seeds nodules, functions soybean nodulation not known. GmWRI1a differently promoted triacylglycerol (TAG) accumulation both Arabidopsis wild‐type wri1...
Abstract The growth of leaves and biosynthesis characteristic secondary metabolites are critically important for tea production quality control. However, little is known about the coordinated regulation leaf development catechin in plants. Here, we reported that TCP TFs involved both development. An integrated analysis profiling CsTCP expression different tissues plants under various environmental conditions at developmental stages indicated significant correlations between transcript levels...
Abstract Soybean has undergone extensive selection pressures for seed nutrient composition and color during domestication, but the major genetic loci controlling coat have not been completely understood, transcriptional regulation relationship among remains elusive. Here, two regulators, GmMYBA2 GmMYBR, were functionally characterized as an anthocyanin activator repressor, respectively. Ectopic expression of in soybean hairy roots conferred enhanced accumulation delphinidin cyanidin types...
As the most well-known and globally consumed central nervous system stimulant, caffeine is a purine alkaloid natural product usually derived from tea coffee. Caffeine has wide range of health benefits on human body, plays crucial roles in pollination, resistance to herbivore attacks, pathogen infections plants (Zhao et al., 2020). While biosynthetic pathways have been extensively studied (Camellia sinensis L) coffee plants, regulation biosynthesis not understood Tea Synthase1 (TCS1) first...
Cold stress declines the quality and yield of tea, yet molecular basis underlying cold tolerance tea plants (Camellia sinensis) remains largely unknown. Here, we identified a circadian rhythm component LUX ARRHYTHMO (LUX) that potentially regulates through genome-wide association study transcriptomic analysis. The expression CsLUX phased with sunrise sunset was strongly induced by stress. Genetic assays indicated is positive regulator freezing in plants. directly activated CsCBF1 repressed...
A large number of terpenoid compounds have been extracted from different tissues S. officinalis. However, the molecular genetic basis terpene biosynthesis pathways is virtually unknown. In this study, approximately 6.6 Gb raw data were generated transcriptome officinalis leaves using Illumina HiSeq 2000 sequencing. After filtering and removing adapter sequences data, reads reached 21 million, comprising 98 million high-quality nucleotide bases. 48,671 unigenes assembled de novo annotated for...
Many terpenoid compounds have been extracted from different tissues of Salvia guaranitica. However, the molecular genetic basis terpene biosynthesis pathways is virtually unknown. In this study, approximately 4 Gb raw data were generated transcriptome S. guaranitica leaves using Illumina HiSeq 2000 sequencing. After filtering and removing adapter sequences data, number reads reached 32 million, comprising 186 million high-quality nucleotide bases. A total 61,400 unigenes assembled de novo...
Malonylated isoflavones are the major forms of isoflavonoids in soybean plants, genes responsible for their biosyntheses not well understood, nor physiological functions. Here we report a new BAHD family isoflavone glucoside malonyltransferase GmIMaT1, and GmIMaT3, which is allelic to previously characterized GmMT7 GmIF7MaT. Biochemical studies showed that recombinant GmIMaT1 GmIMaT3 enzymes used malonyl-CoA several 7-O-glucosides as substrates. The Km values glycitin, genistin daidzin were...
Despite of important functions strigolactones (SLs) and karrikins (KARs) in plant development, plant-parasite plant-fungi interactions, their roles soybean-rhizobia interaction remain elusive. SL/KAR signaling genes GmMAX2a, GmD14s, GmKAIs are activated by rhizobia infection. GmMAX2a restored atmax2 root hair defects soybean hairs were changed overexpression (GmMAX2a-OE) or knockdown (GmMAX2a-KD) mutants. GmMAX2a-KD gave fewer, whereas GmMAX2a-OE produced more nodules than GUS hairy roots....
Tea (Camellia sinensis L.) leaves synthesize and concentrate a vast array of galloylated catechins (e.g., EGCG ECG) non-galloylated EGC, catechin, epicatechin), together constituting 8 %-24 % the dry leaf mass. Galloylated account for major portion soluble in tea (up to 75%) make contribution astringency bitter taste green tea, their pharmacological activity human health. However, catechin galloylation mechanism plants is largely unknown at molecular levels. Previous studies indicated that...
Theanine is a unique major amino acid in tea plants responsible for umami taste and mental health benefits of tea. However, theanine biosynthesis physiological role are not fully understood. Here, we demonstrate that plant synthetase encoded by glutamine gene CsTSI. The expression pattern CsTSI closely correlated with levels various tissues. transcripts were accumulated root tip epidermal cells, pericycle procambial where presents as cytosolic protein. Ectopic the Arabidopsis led to greater...
The WRKY gene family is most widely known as being the key plant transcription factor involved in various stress responses and affecting growth development. In this study, a total of 86 members <italic>CsWRKY</italic> genes were identified from tea genome. Most these contain several important <italic>Cis</italic>-regulatory elements promoter regions associated with multiple stress-responses. These further classified into three groups, I, II, III, each 21, 58, 7 members, respectively. We...
Caffeine is a characteristic bioactive compound in tea and coffee plants, which synthesized accumulated extensively leaves seeds. However, little known about the regulatory mechanism of caffeine synthesis plants. This study compared metabolite between We found that contained significantly higher than leaves, perhaps due to more members N-methyltransferase (NMT) genes as well expression levels Substantial numbers transcription factors were predicted be involved biosynthesis regulation,...