A5052947560- Inflammatory Bowel Disease
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- SARS-CoV-2 detection and testing
- Celiac Disease Research and Management
- Gut microbiota and health
- Eosinophilic Esophagitis
- Clinical Nutrition and Gastroenterology
- SARS-CoV-2 and COVID-19 Research
- Digestive system and related health
- Barrier Structure and Function Studies
- Biosensors and Analytical Detection
- Autoimmune and Inflammatory Disorders
- Asthma and respiratory diseases
- COVID-19 Clinical Research Studies
- Drug Transport and Resistance Mechanisms
- Gastroesophageal reflux and treatments
- COVID-19 diagnosis using AI
- IL-33, ST2, and ILC Pathways
- Advanced biosensing and bioanalysis techniques
- Clostridium difficile and Clostridium perfringens research
- Gastrointestinal disorders and treatments
- Diet and metabolism studies
- Gastric Cancer Management and Outcomes
- Liver Disease Diagnosis and Treatment
University of Calgary
2016-2025
Alberta Health Services
2022-2024
University of Alberta
1996-2022
University of Waterloo
2022
Alberta Bible College
2018
Health Sciences Centre
2007-2017
Institute of Infection and Immunity
2013
World Water Watch
2011
Vehicle Technologies Office
2009
Royal Alexandra Hospital
2007
Increased intestinal permeability (IP) has emerged recently as a common underlying mechanism in the pathogenesis of allergic, inflammatory, and autoimmune diseases. The characterization zonulin, only physiological mediator known to regulate IP reversibly, remained elusive. Through proteomic analysis human sera, we have now identified zonulin precursor for haptoglobin-2 (pre-HP2). Although mature HP is scavenge free hemoglobin (Hb) inhibit its oxidative activity, no function ever been...
The addition of spermine or spermidine to rat liver assay systems produced marked changes in a number microsomal drug oxidations. hydroxylation aniline and the N-demethylation ethylmorphine were both enhanced with concentrations 1-10 mM spermidine. results putrescine on metabolism less dramatic, no effect could be observed studies other substrates. In contrast enhancing effects, inhibition was when added p-nitroanisole O-demethylation mixtures, assays for acetanilide hydroxylation....
INTRODUCTION: Nonalcoholic fatty liver disease (NAFLD) is an obesity-related disorder that rapidly increasing in incidence and considered the hepatic manifestation of metabolic syndrome. The gut microbiome plays a role metabolism maintaining barrier integrity. Studies have found differences microbiota between NAFLD healthy patients increased intestinal permeability with NAFLD. Fecal transplantation (FMT) can be used to alter microbiome. It was hypothesized FMT from thin donor given would...
Summary Background : Post‐infectious irritable bowel syndrome is a common clinical phenomenon of uncertain aetiology. Aim To test the association between intestinal permeability and symptoms 2 years after large waterborne outbreak bacterial gastroenteritis. Methods Consecutive adults with Rome I controls without attending community clinic were enrolled. Intestinal was measured as ratio fractional urinary excretions lactulose mannitol, compared among cases vs. predictors abnormal assessed....
Summary Background Lifelong adherence to a strict gluten‐free diet is the cornerstone of coeliac disease treatment. Elucidation pathogenesis has created opportunities for novel therapeutic approaches disease. AT‐1001 an inhibitor paracellular permeability whose structure derived from protein secreted by Vibrio cholerae . Aim To determine safety and tolerability 12 mg doses in subjects challenged with gluten. Methods An in‐patient, double‐blind, randomized placebo‐controlled study utilizing...
To determine whether treatment with a potent protease-resistant analog of human glucagon-like peptide 2 (GLP-2) might augment the adaptive response to massive intestinal resection, rats were divided into resected, which had 75% midjejunoileum removed, sham-resected, and nonsurgical groups. Within each group, animals assigned 21 days drug (0.1 μg/g GLP-2 in phosphate-buffered saline) or vehicle alone subcutaneously twice daily. Food intake; weight gain; jejunal ileal diameters, total mucosal...
Crohn's disease (CD) is a chronic relapsing inflammatory bowel disorder. Both biological and psychosocial factors may modulate the illness experience.The aim of this study was to identify clinical, parameters as predictors clinical relapse in quiescent CD.Patients medically induced remission were followed prospectively for 1 year, or less if they relapsed. Disease characteristics determined at baseline. Serum cytokines, anti-Saccharomyces cerevisiae antibodies, C-reactive protein (CRP),...
Background/Aims: The aim of this exploratory trial was to establish if the probiotic Bifidobacterium natren life start (NLS) strain may affect clinical course and pathophysiological features patients with untreated celiac disease (CD). Positive findings would be helpful in directing future studies. Methods: Twenty-two adult having 2 positives CD-specific tests were enrolled. Patients randomized receive capsules before meals for 3 weeks either infantis super (Lifestart 2) (2×109...
Vitamin D (vitD) supplementation may prolong remission in Crohn's disease (CD); however, the clinical efficacy and mechanisms are unclear.To determine changes intestinal permeability (IP), antimicrobial peptide (AMP) concentrations markers CD, response to vitD supplementation.In a double-blind randomised placebo-controlled study, we assigned 27 CD patients 2000 IU/day or placebo for 3 mos. We determined IP, plasma cathelicidin (LL-37 ng/mL), human-beta-defensin-2 (hBD2 pg/mL), activity...
Abstract Context Patients with type 1 diabetes (T1D) have lower microbiota diversity and distinct gut microbial profiles that been linked to changes in intestinal permeability. Prebiotics are nondigestible carbohydrates alter could potentially improve glycemic control reduce permeability thereby insulin sensitivity. Objective To determine the effect of prebiotics on control, microbiota, children T1D. Design A randomized, placebo-controlled trial 8 17 years age T1D using placebo or prebiotic...
SARS-CoV-2 has been detected in wastewater and its abundance correlated with community COVID-19 cases, hospitalizations deaths. We sought to use wastewater-based detection of assess the epidemiology hospitals. Between August December 2020, twice-weekly samples from three tertiary-care hospitals (totaling > 2100 dedicated inpatient beds) were collected. Hospital-1 Hospital-2 could be captured a single sampling point whereas Hospital-3 required separate monitoring sites. Wastewater...
Patients with MS have an altered gut microbiota compared to healthy individuals, as well elevated small intestinal permeability, which may be contributing the development and progression of disease.We sought investigate if fecal transplantation was safe tolerable in patients it could improve abnormal permeability.Nine were recruited provided monthly FMTs for up six months. The primary outcome investigated change peripheral blood cytokine concentrations. secondary outcomes composition, safety...
Wastewater-based epidemiology (WBE) is an emerging surveillance tool that has been used to monitor the ongoing COVID-19 pandemic by tracking SARS-CoV-2 RNA shed into wastewater. WBE was performed occurrence and spread of from three wastewater treatment plants (WWTP) six neighborhoods in city Calgary, Canada (population 1.44 million). A total 222 WWTP 192 neighborhood samples were collected June 2020 May 2021, encompassing end first-wave (June 2020), second-wave (November December 2020)...
Increased small intestinal permeability is a longstanding observation in both Crohn's disease patients and their healthy, asymptomatic first‐degree relatives. However, the significance of this compromised gut barrier function its place pathogenesis remains poorly understood. The association between abnormal specific mutation NOD2 gene, which functions to modulate innate adaptive immune responses bacteria, suggests common, genetically determined pathway by an could result chronic...
Confocal endomicroscopy can be used to image intestinal mucosa. Epithelial gaps resulting from shedding of epithelial cells have been reported in patients. We hypothesize that the rate cell increases patients with Crohn's disease, leading more and barrier dysfunction. In this study, we probe-based confocal laser quantify disease compared controls. also determined density a mouse model inflammatory bowel disease-interleukin-10-deficient (IL-10) mice, versus background strain using rigid probe...
Abstract Background Children with food allergy have been shown to increased small intestinal permeability ( IP ) following ingestion of the offending as well during elimination diets. We investigated in asymptomatic allergic children an diet identify clinical characteristics associated altered . Methods Urinary recovery ratios lactulose and mannitol L / M were determined 5 h 7.5 g 2 131 cow's milk egg children. An ratio ≥0.025 was considered abnormal based upon previously established...