A5047150575- Gastrointestinal motility and disorders
- Diet and metabolism studies
- Gut microbiota and health
- Adenosine and Purinergic Signaling
- Helicobacter pylori-related gastroenterology studies
- Infant Health and Development
- Congenital gastrointestinal and neural anomalies
- Gastroesophageal reflux and treatments
- Neuropeptides and Animal Physiology
- Child Nutrition and Feeding Issues
- Tryptophan and brain disorders
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolites in Food Biotechnology
- Restless Legs Syndrome Research
- Clinical Nutrition and Gastroenterology
- GDF15 and Related Biomarkers
- Whipple's Disease and Interleukins
- Seaweed-derived Bioactive Compounds
- Frailty in Older Adults
- Ginger and Zingiberaceae research
- Biochemical and Molecular Research
- Neuroscience of respiration and sleep
- Vagus Nerve Stimulation Research
- Bipolar Disorder and Treatment
- Therapeutic Uses of Natural Elements
United States Department of Agriculture
2025
Agricultural Research Service
2025
University College Cork
2018-2024
APC Microbiome Institute
2018-2024
University of Arkansas at Fayetteville
2021-2022
University of Padua
2013-2022
Istituto Oncologico Veneto
2017
Istituti di Ricovero e Cura a Carattere Scientifico
2017
Background and Purpose Gut microbiota is essential for the development of gastrointestinal system, including enteric nervous system (ENS). Perturbations gut in early life have potential to alter neurodevelopment leading functional bowel disorders later life. We examined hypothesis that dysbiosis impairs structural integrity ENS, dysmotility juvenile mice. Experimental Approach To induce dysbiosis, broad‐spectrum antibiotics were administered by gavage (3weeks old) male C57Bl/6 mice 14 days....
Antibiotic use during adolescence may result in dysbiosis-induced neuronal vulnerability both the enteric nervous system (ENS) and central (CNS) contributing to onset of chronic gastrointestinal disorders, such as irritable bowel syndrome (IBS), showing significant psychiatric comorbidity. Intestinal microbiota alterations influence expression molecular factors involved development ENS CNS. In this study, we have evaluated brain-derived neurotrophic factor (BDNF) its high-affinity receptor...
Recent evidence indicates an involvement of P2X7 purinergic receptor (P2X7R) in the fine tuning immune functions, as well driving enteric neuron apoptosis under intestinal inflammation. However, participation this regulation neuromuscular functions remains undetermined. This study was aimed at investigating role P2X7Rs control colonic motility experimental colitis.Colitis induced rats by 2,4-dinitrobenzenesulfonic acid. P2X7R distribution examined immunofluorescence analysis. The effects...
Edible seaweeds have been consumed by Asian coastal communities since ancient times.
Objective Toll-like receptors (TLRs) play a pivotal role in the homeostatic microflora-host crosstalk. TLR4-mediated modulation of both motility and enteric neuronal survival has been reported mainly for colon with limited information on TLR4 tuning structural functional integrity nervous system (ENS) controlling small bowel motility. Methods Male knockout (TLR4-/-, 9±1 weeks old) sex- age-matched wild-type (WT) C57BL/6J mice were used experiments. Alterations ENS morphology neurochemical...
The present study was designed to examine the role of enteric glial cells (EGCs) in colonic neuromuscular dysfunctions a mouse model high-fat diet (HFD)-induced obesity. C57BL/6J mice were fed with HFD or standard (SD) for 1, 2, 8 weeks. Colonic interleukin (IL)-1β, IL-6, and malondialdehyde (MDA) levels measured. Expression occludin tissues examined by western blot. Substance P (SP), S100β, GFAP, phosphorylated mitogen-activated protein kinase 1 (pERK) assessed whole mount specimens plexus...
Background We evaluated the effect of Saccharomyces boulardii CNCM I-745 on intestinal neuromuscular anomalies in an IBS-type mouse model gastrointestinal motor dysfunctions elicited by Herpes Simplex Virus type 1 (HSV-1) exposure. Methods Mice were inoculated intranasally with HSV-1 (102 PFU) or vehicle at time 0 and 4 weeks later intragastric (IG) route (108 PFU). Six after IG inoculum, mice randomly allocated to receive oral gavage either S. (107 CFU/day) vehicle. After following...
The role played by adenosine A2B receptors (A2BRs) in the regulation of enteric glial cell (EGC) functions remains unclear. This study was aimed at investigating involvement A2BRs control EGC a model obesity. C57BL/6 mice were fed with standard diet (SD) or high fat (HFD) for eight weeks. Colonic tachykininergic contractions recorded presence BAY60-6583 (A2BRs agonist), MRS1754 antagonist), and gliotoxin fluorocitrate. Immunofluorescence distribution HuC/D, S100β, assessed whole mount...
Nutritional approaches have emerged over the past number of years as suitable interventions to ameliorate enduring effects early life stress. Maternal separation (MS) is a rodent model stress which induces widespread changes across microbiota-gut-brain axis. Milk fat globule membrane (MFGM) neuroactive structure that surrounds milk globules in breast and has been shown positive health infants, yet mechanisms behind this are not fully known. Here, we investigated MFGM supplementation from...
The developing central nervous system is highly sensitive to nutrient changes during the perinatal period, emphasising potential impact of alterations maternal diet on offspring brain development and behaviour. A growing body research implicates gut microbiota in neurodevelopment Maternal overweight obesity period has been linked neurodevelopment, plasticity affective disorders offspring, with implications for microbial signals from gut. Here we investigate high-fat (mHFD)-induced...
Adenosine A(2B) receptors regulate several physiological enteric functions. However, their role in the pathophysiology of intestinal dysmotility associated with inflammation has not been elucidated. Hence, we investigated expression A2B rat colon and control cholinergic motility presence bowel inflammation.Colitis was induced by 2,4-dinitrobenzenesulfonic acid (DNBS). Colonic receptor localization were examined RT-PCR immunofluorescence. The interaction between adenosine deaminase assayed...
Enteric glial cells (EGCs) influence nitric oxide (NO)− and adenosine diphosphate (ADP)− mediated signaling in the enteric nervous system (ENS). Since Toll-like receptor 4 (TLR4) participates to EGC homoeostasis, this study aimed evaluate possible involvement of EGCs alterations inhibitory neurotransmission TLR4−/− mice. Ileal segments from male wild-type (WT) C57BL/6J mice were incubated with gliotoxin fluoroacetate (FA). Alterations ENS morphology neurochemical coding investigated by...
Abstract Background and Purpose Inflammatory bowel disease (IBD) patients display genetic polymorphisms in toll‐like receptor 4 (TLR4) genes, contributing to dysregulate enteric nervous system (ENS) circuits with increased levels of 5‐HT alteration the neuroimmune crosstalk. In this study, we investigated impact TLR4 signalling on mouse ENS dysfunction caused by dextran sulphate sodium (DSS)‐induced ileitis. Experimental Approach Male C57BL/6J (wild‐type [WT]) −/− mice (10 ± 2 weeks old)...
Antidopaminergic gastrointestinal prokinetics are indeed commonly used to treat motility disorders, although the precise role of dopaminergic transmission in gut is still unclear. Since dopamine transporter (DAT) involved several brain disorders by modulating extracellular central nervous system, this study evaluated impact DAT genetic reduction on morpho-functional integrity mouse small intestine enteric system (ENS). In heterozygous (DAT+/−) and wild-type (DAT+/+) mice (14 ± 2 weeks)...
Background and Purpose Enteric neurogenic/inflammation contributes to bowel dysmotility in obesity. We examined the role of NLRP3 colonic neuromuscular dysfunctions mice with high‐fat diet (HFD)‐induced Experimental Approach Wild‐type C57BL/6J NLRP3‐KO ( Nlrp3 −/− ) were fed HFD or standard for 8 weeks. The activation inflammasome pathways tissues from obese was assessed. vivo transit vitro tachykininergic contractions substance P distribution evaluated. effect on signalling tested cultured...
Oxidized phospholipid derivatives (OxPAPCs) act as bacterial lipopolysaccharide (LPS)-like damage-associated molecular patterns. OxPAPCs dose-dependently exert pro- or anti-inflammatory effects by interacting with several cellular receptors, mainly Toll-like receptors 2 and 4. It is currently unknown whether may affect enteric nervous system (ENS) functional structural integrity.Juvenile (3 weeks old) male C57Bl/6 mice were treated intraperitoneally OxPAPCs, twice daily for 3 days. Changes...