A5035468956- Cancer Cells and Metastasis
- Ion Channels and Receptors
- Epigenetics and DNA Methylation
- Ion channel regulation and function
- Neuroendocrine regulation and behavior
- Cancer, Hypoxia, and Metabolism
- Adenosine and Purinergic Signaling
- Bioactive Compounds and Antitumor Agents
- Neonatal Respiratory Health Research
- Drug Transport and Resistance Mechanisms
- Neuroscience of respiration and sleep
- Chemical Reactions and Isotopes
- Neurobiology and Insect Physiology Research
- Estrogen and related hormone effects
- Wnt/β-catenin signaling in development and cancer
- 3D Printing in Biomedical Research
- Protein Kinase Regulation and GTPase Signaling
- Bone Metabolism and Diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Advanced Fluorescence Microscopy Techniques
- Erythrocyte Function and Pathophysiology
- Animal Genetics and Reproduction
- Metabolism, Diabetes, and Cancer
- MicroRNA in disease regulation
- Ion Transport and Channel Regulation
The University of Queensland
2014-2024
UNSW Sydney
2021-2024
Aarhus University
2021-2024
EMBL Australia
2021-2023
National Institute of Environmental Health Sciences
2016-2023
Institute of Health Visiting
2023
Translational Research Institute
2016-2021
Mater Research
2016-2021
University of Cambridge
2015-2018
National Institutes of Health
2013-2016
Abstract The entry of calcium into the mammary epithelial cell from maternal plasma (i.e., influx mechanisms) during lactation is poorly understood. As alterations in channels and pumps are a key feature some cancers, including breast cancer, understanding these pathways may have significance beyond biology. We show that store-operated protein, Orai1, increased whereas Orai1 activator Stim1, but not Stim2, downregulated. Stim2 siRNA reduced basal levels model. Our results suggest remodeled...
Abstract The mammary gland undergoes cycles of growth and regeneration throughout reproductive life, a process that requires stem cells (MaSCs). Whilst recent genetic fate-mapping studies using lineage-specific promoters have provided valuable insights into the epithelial hierarchy, true differentiation potential adult MaSCs remains unclear. To address this, herein we utilize stochastic genetic-labelling strategy to indelibly mark single cell its progeny in situ , combined with tissue...
High-resolution 3D imaging of intact tissue facilitates cellular and subcellular analyses complex structures within their native environment. However, difficulties associated with immunolabelling fluorescent proteins deep whole organs have restricted applications to thin sections or processed preparations, precluding comprehensive rapid visualisation. Several clearing methods been established circumvent issues depth in opaque specimens. The application these techniques study the elaborate...
Homozygous mutation of the Csf1r locus ( Csf1rko ) in mice, rats and humans leads to multiple postnatal developmental abnormalities. To enable analysis mechanisms underlying phenotypic impacts mutation, we bred a rat allele inbred dark agouti (DA) genetic background -mApple reporter transgene. The led almost complete loss embryonic macrophages ablation most adult tissue macrophage populations. We extended previous phenotype early development reveal on musculoskeletal proliferation...
In addition to their well-defined roles in replenishing depleted endoplasmic reticulum (ER) Ca2+ reserves, molecular components of the store-operated entry pathway regulate breast cancer metastasis. A process implicated metastasis that describes conversion a more invasive phenotype is epithelial-mesenchymal transition (EMT). this study we show EGF-induced EMT MDA-MB-468 cells associated with reduction agonist-stimulated and influx, prior induction have high level non-stimulated influx. The...
Significance All mammals, from platypuses to humans, produce relatively immature offspring that are wholly dependent on their mother’s milk for postnatal growth and development. However, the dynamic signaling molecular mechanisms responsible transport of key constituents (e.g., calcium) into alveolar unit contraction ejection not fully understood. Using genetically modified mouse models, we demonstrate store-operated Ca 2+ channel Orai1 delivers over 50% calcium ions present in milk. We also...
Hypoxia is a feature of the microenvironment many cancers and can trigger epithelial-mesenchymal transition (EMT), process by which cells acquire more invasive phenotype with enriched survival. A remodeling adenosine 5'-triphosphate (ATP)-induced Ca(2+) signaling via purinergic receptors associated epidermal growth factor (EGF)-induced EMT in MDA-MB-468 breast cancer cells. Here, we assessed ATP-mediated model hypoxia-induced Like EGF, hypoxia treatment (1% O2) was also significant reduction...
Mammary gland development begins in the embryo and continues throughout reproductive life of female mammals. Tissue macrophages (Mϕs), dependent on signals from Mϕ colony stimulating factor 1 receptor (CSF1R), have been shown to regulate generation, regression regeneration this organ, which is central for mammalian offspring survival. However, distribution Mϕs pre- post-natal mammary gland, as it undergoes distinct phases regression, unknown or has inferred immunostaining thin tissue...
Errors in clinical decision-making are disturbingly common. Here, we show that structured information–sharing networks among clinicians significantly reduce diagnostic errors, and improve treatment recommendations, as compared to groups of ...Errors Recent studies have found 10 15% all decisions regarding diagnoses inaccurate. experimentally study the ability ...
Abstract Background Epithelial-mesenchymal transition (EMT) is a process implicated in cancer metastasis that involves the conversion of epithelial cells to more mesenchymal and invasive cell phenotype. In breast EMT associated with altered store-operated calcium influx changes signalling mediated by activation surface purinergic receptors. this study, we investigated whether MDA-MB-468 induced undergo exhibit mRNA levels channels, pumps exchangers located on intracellular storing...
Background The microenvironment plays a pivotal role in tumor cell proliferation, survival and migration. Invasive cancer cells face new set of environmental challenges as they breach the basement membrane colonize distant organs during process metastasis. Phenotypic switching, such that which occurs epithelial-mesenchymal transition (EMT), may be associated with remodeling surface receptors thus altered responses to signals from microenvironment. Methodology/Principal Findings We assessed...
ABSTRACT Mammary gland development occurs over multiple phases, beginning in the mammalian embryo and continuing throughout reproductive life. The remarkable morphogenetic capacity of mammary at each stage is attributed to activities distinct populations stem cells (MaSCs) progenitor cells. However, relationship between embryonic adult MaSCs, their fate during different waves morphogenesis, remains unclear. By employing a neutral, low-density genetic labelling strategy, we characterised...
Lacrimal glands function to produce an aqueous layer, or tear film, that helps nourish and protect the ocular surface. secrete proteins, electrolytes water, loss of gland can result in film disorders such as dry eye syndrome, a widely encountered debilitating disease ageing populations. To combat these disorders, understanding underlying molecular signalling processes control lacrimal will give insight into corrective therapeutic approaches. Previously, single cells isolated from glands, we...
ABSTRACT The ability of a mother to produce nutritionally complete neonatal food source has provided powerful evolutionary advantage mammals. Milk production by mammary epithelial cells is adaptive, its release exquisitely timed, and own glandular stagnation with the permanent cessation suckling triggers cell death tissue remodeling that enables female mammals nurse successive progeny. Chemical mechanical signals both play role in this process. However, despite duality input, much remains...