A5102978981- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- Infectious Diseases and Tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Diagnosis and treatment of tuberculosis
- Immunodeficiency and Autoimmune Disorders
- Respiratory viral infections research
- Emergency and Acute Care Studies
- Nosocomial Infections in ICU
- Chronic Disease Management Strategies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Sepsis Diagnosis and Treatment
- Clostridium difficile and Clostridium perfringens research
- Cystic Fibrosis Research Advances
- Pain Mechanisms and Treatments
- Diabetes and associated disorders
- Actinomycetales infections and treatment
- Venous Thromboembolism Diagnosis and Management
- Inhalation and Respiratory Drug Delivery
- Myasthenia Gravis and Thymoma
- Orthopedic Infections and Treatments
Odense University Hospital
2012-2025
University of Copenhagen
2012-2024
Gentofte Hospital
1998-2024
Haukeland University Hospital
2022
Regionshospitalet Silkeborg
2017-2020
Kolding Hospital
2019
Nordsjællands Hospital
2009-2017
Copenhagen University Hospital
2007-2017
Zealand University Hospital
2012-2017
University College Capital
2012-2017
Tuberculosis control relies on the identification and preventive treatment of individuals who are latently infected with Mycobacterium tuberculosis . However, direct latent infection is not possible. The diagnostic tests used to identify M. , in vivo tuberculin skin test ex interferon-γ release assays (IGRAs), designed an adaptive immune response against, but necessarily a with, proportion truly remain after or IGRA conversion unknown. It also uncertain how long responses towards...
Anti-tumour necrosis factor (TNF) monoclonal antibodies or soluble TNF receptors have become an invaluable treatment against chronic inflammatory diseases, such as rheumatoid arthritis, bowel disease and psoriasis. Individuals who are treated with antagonists at increased risk of reactivating latent infections, especially tuberculosis (TB). Following antagonist therapy, the relative for TB is up to 25 times, depending on clinical setting used. Interferon-γ release assays or, alternative in...
In the absence of active tuberculosis, a positive tuberculin skin test (TST) or interferon-γ release assay (IGRA) result defines latent infection with Mycobacterium although results may vary depending on immunodeficiency.This study compared performance TST and IGRAs in five different groups immunocompromised patients, evaluated their ability to identify those at risk for development tuberculosis.Immunocompromised patients HIV infection, chronic renal failure, rheumatoid arthritis,...
Background A basic paradigm of human infection is that acute bacterial disease caused by fast growing planktonic bacteria while chronic infections are slow-growing, aggregated bacteria, a phenomenon known as biofilm. For lung infections, this has been thought to be supported observations how proliferate in well-established growth media the laboratory—the gold standard microbiology. Objective To investigate architecture sputum from patients with and infections. Methods Advanced imaging...
ABSTRACT Tests based on tuberculin purified protein derivative (PPD) cannot distinguish between tuberculosis infection, Mycobacterium bovis BCG vaccination, or exposure to environmental mycobacteria. The present study investigated the diagnostic potential of two -specific antigens (ESAT-6 and CFP10) in experimental animals as well during natural infection humans cattle. Both were frequently recognized vivo vitro induction delayed-type hypersensitivity responses ability induce gamma...
Human T cell responses to ESAT-6 and eight synthetic overlapping peptides were investigated in tuberculosis (TB) patients control subjects from regions of high low endemicity for TB. was recognized by 65% all tuberculin purified protein derivative—responsive TB patients, whereas only 2 29 bacille Calmette-Guérin—vaccinated Danish healthy donors this molecule. In Ethiopia, a frequency (58%) contacts ESAT-6. All the some donors, indicating that molecule holds multiple epitopes. Ethiopian...
ABSTRACT Culture filtrate from Mycobacterium tuberculosis contains protective antigens of relevance for the generation a new antituberculosis vaccine. We have identified two previously uncharacterized M. proteins (TB7.3 and TB10.4) highly active low-mass fraction culture filtrate. The molecules were characterized, mapped in two-dimensional electrophoresis reference map short-term filtrate, compared with another recently protein, CFP10 (F. X. Berthet, P. B. Rasmussen, I. Rosenkrands,...
ABSTRACT Diagnosis of latent Mycobacterium tuberculosis infection is considered essential for control but hampered by the lack specific reagents. We report that strong recognition complex-specific antigen ESAT-6 healthy household contacts patients correlates with subsequent development active during a 2-year follow-up period.
Abstract Background Although tuberculosis (TB) is a minor problem in Denmark, severe and complicated cases occur HIV positive. Since the new M. specific test for latent TB, QuantiFERON-TB In-Tube (QFT-IT) became available patients our clinic have been screened presence of TB using QFT-IT test. We here report results from first screened. Methods On routine basis was performed 590 positive individuals consecutively tested are presented here. CD4 cell count risk-factors were recorded patient...
ABSTRACT A new immunodiagnostic test based on the Mycobacterium tuberculosis -specific antigens CFP-10/ESAT-6(QFT-RD1) has been launched as an aid in diagnosis of latent (TB) infection (LTBI). The aim this study was to evaluate for active TB. Eighty-two patients with suspicion TB and 39 healthy BCG-vaccinated persons were enrolled. Forty-eight had TB, 25 did not, 9 excluded. Sensitivity specificity evaluated a prospective blinded manner suspected sensitivity QFT-RD1 85% (40/48; confidence...
The aim of the present study was to evaluate potential diagnostic tests based on interferon-γ inducible protein (IP)-10 and monocyte chemotactic (MCP)-2, compare performance with QuantiFERON TB® Gold In-Tube (QFT-IT; Cellestis, Carnagie, Australia) test. IP-10 MCP-2 were determined in supernatants from whole blood stimulated <i>Mycobacterium tuberculosis</i>-specific antigens. Samples obtained 80 patients culture- and/or PCR-proven tuberculosis (TB), 124 unexposed healthy controls: 86 high...
During screening for latent tuberculosis infection (LTBI), before anti-tumor-necrosis-factor-α treatment, most patients are already receiving immunosuppressive therapy. The objective was to evaluate the performance of QuantiFERON Gold In-Tube (QFT-IT) and Tuberculin Skin Test (TST).A prospective multicenter study included 248 with ulcerative colitis (39), Crohn's disease (54), rheumatoid arthritis (111), spondylo-arthropathy (44).QFT-IT positive in 7/248 (3%), negative 229 (92%),...
There are limited data on outcomes of moderate to severe coronavirus disease 2019 (COVID-19) among patients treated with remdesivir and dexamethasone in a real-world setting. We sought compare the effectiveness standard care (SOC) alone versus SOC plus dexamethasone.Two population-based nationwide cohorts individuals hospitalized COVID-19 during February through December 2020 were studied. Death within 30 days need mechanical ventilation (MV) compared by inverse probability treatment...
Abstract Lack of rapid and comprehensive microbiological diagnosis in patients with community acquired pneumonia (CAP) hampers appropriate antimicrobial therapy. This study evaluates the real-world performance BioFire FilmArray Pneumonia panel plus (FAP ) explores feasibility evaluation a randomised controlled trial. Patients presenting to hospital suspected CAP were recruited prospective study. An induced sputum or an endotracheal aspirate was obtained from all participants. The FAP...
Many aspects of the widely used bacillus Calmette-Guérin (BCG) vaccine against tuberculosis are still subject controversy. There is a huge variation in efficacy from one clinical trial to another and no relationship between vaccine-induced skin test conversion subsequent protection. We have studied vitro cell-mediated immune responses primed by BCG vaccination 22 healthy Danish donors with different levels purified protein derivative (PPD) reactivity before vaccination. The study...
Abstract Contacts of adults with tuberculosis (TB) are at risk for infection. Tests based on interferon-γ (IFN-γ) expression in response to Mycobacterium antigens may be more sensitive than the tuberculin skin test (TST). Risk infection was assessed by using TST and an IFN-γ-based assay (QuantiFERON Gold Tube [QFT-IT] test) 207 children Nigeria 1 3 groups: contact smear-positive TB, smear-negative controls. For these groups, respectively, results were >10 mm 38 (49%) 78, 13 (16%) 83, 6 (13%)...
We have screened peripheral blood mononuclear cells (PBMC) from tuberculosis (TB) patients for proliferative reactivity and interferon-gamma (IFN-gamma) secretion against a panel of purified recombinant (r) natural (n) culture filtrate (rESAT-6, nMPT59, nMPT64 nMPB70) somatic-derived (rGroES, rPstS, rGroEL rDnaK) antigens Mycobacterium tuberculosis. The responses PBMC to these defined were compared with the corresponding results obtained complex antigens, such as whole-cell M. tuberculosis,...
ABSTRACT The currently used method for immunological detection of tuberculosis infection, the tuberculin skin test, has low specificity. Antigens specific Mycobacterium to replace purified protein derivative are therefore urgently needed. We have performed a rigorous assessment diagnostic potential four recently identified antigens (Rv2653, Rv2654, Rv3873, and Rv3878) from genomic regions that lacking bovis bacillus Calmette-Guérin (BCG) vaccine strains as well most common nontuberculous...
IFN-gamma responses to M. tuberculosis antigens are used as in-vitro diagnostic tests for infection. The highly specific but sensitivity may be impaired due immuno-suppression. objective of this small exploratory study was compare three novel biomarkers diagnosis - MCP-1, MCP-3 and IL-1RA with the current established biomarker newly described IP-10 MCP-2.Whole blood from 8 patents active 7 healthy controls stimulated mitogen in Quantiferon In Tube test tubes. Levels were measured using...
There is a need for simple tools such as the M.tuberculosis specific IFN-gamma release assays (IGRA) to improve diagnosis of M.tuberculosis-infection in children. The aim study was evaluate performance an IP-10 and IL-2 based tests recently exposed children from Nigeria.Samples were obtained 59 at high risk infection with (contacts adults smear culture-positive tuberculosis) 61 low smear-negative/culture-positive tuberculosis or community controls). measured plasma after stimulation...