A5077329616- Microplastics and Plastic Pollution
- Photoreceptor and optogenetics research
- Cancer-related molecular mechanisms research
- Photosynthetic Processes and Mechanisms
- biodegradable polymer synthesis and properties
- Advanced NMR Techniques and Applications
- Light effects on plants
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Ferroptosis and cancer prognosis
- Polymer crystallization and properties
- Traditional Chinese Medicine Analysis
- Estrogen and related hormone effects
- Bone Metabolism and Diseases
- Sarcoma Diagnosis and Treatment
- Extracellular vesicles in disease
- Cancer Research and Treatments
- Photochromic and Fluorescence Chemistry
- MicroRNA in disease regulation
- Healthcare and Venom Research
- Effects and risks of endocrine disrupting chemicals
- Pharmacological Effects of Natural Compounds
- Bone health and treatments
- Recycling and Waste Management Techniques
Central South University
2022-2024
Second Xiangya Hospital of Central South University
2022-2024
Leipzig University
2020-2024
Earth is flooded with plastics and the need for sustainable recycling strategies polymers has become increasingly urgent. Enzyme-based hydrolysis of post-consumer plastic an emerging strategy closed-loop polyethylene terephthalate (PET). The polyester hydrolase PHL7, isolated from a compost metagenome, completely hydrolyzes amorphous PET films, releasing 91 mg terephthalic acid per hour enzyme. Vertical scanning interferometry shows degradation rates film 6.8 μm h
Abstract The recently discovered metagenomic-derived polyester hydrolase PHL7 is able to efficiently degrade amorphous polyethylene terephthalate (PET) in post-consumer plastic waste. We present the cocrystal structure of this with its hydrolysis product terephthalic acid and elucidate influence 17 single mutations on PET-hydrolytic activity thermal stability PHL7. substrate-binding mode similar that thermophilic LCC deviates from mesophilic Is PETase. subsite I modifications L93F Q95Y,...
Background: Osteoarthritis (OA) is a degenerative disease which serious affects patients. Ligusticum chuanxiong (CX) has been shown to have certain curative effect on osteoarthritis in traditional Chinese medicine therapy. This study based network pharmacology and molecular docking technology explore the potential mechanism of CX. Methods: Components CX treat were screened TCMSP database targets predicted by PharmMapper database, collected from GeneCards intersection genes found be possible...
Enzymatic degradation of polyethylene terephthlate (PET) by polyester hydrolases is currently subject to intensive research, as it considered a potential eco-friendly recycling method for plastic waste. However, the substrate-binding mode and molecular mechanism enzymatic PET hydrolysis are still under intense investigation, controversial hypotheses have been presented. To help unravel inherent biocatalytic at atomic level, we performed solid-state NMR measurements cutinase from Thermobifida...
Abstract Background Long non-coding RNA X-inactive specific transcript (XIST) regulates the progression of a variety tumors, including osteosarcoma. Bone marrow mesenchymal stem cells (BMSCs) can be recruited into osteosarcoma tissue and affect by secreting exosomes. However, whether BMSCs derived exosomes transmit XIST to regulate growth metastasis related mechanism are still unclear. Method In this study, were used treat human MG63 143B, level in was intervened siRNA. CCK-8, EdU, transwell...
The solid-state photo-chemically induced dynamic nuclear polarization (photo-CIDNP) effect generates non-Boltzmann spin magnetization, referred to as hyperpolarization, allowing for high gain of sensitivity in magnetic resonance (NMR). Well known occur photosynthetic reaction centers, the was also observed a light-oxygen-voltage (LOV) domain blue-light receptor phototropin, which functional cysteine removed prevent photo-chemical reactions with cofactor, flavin mononucleotide (FMN). Upon...
In blue-light sensing LOV domains tryptophans and tyrosines near the flavin chromophore dramatically influence triplet state decay, efficiency of singlet oxygen production yield photoproduct.
Background: The GINS complex, composed of GINS1/2/3/4 subunits, is an essential structure Cdc45-MCM-GINS (CMG) helicase and plays a vital role in establishing the DNA replication fork chromosome replication. Meanwhile, genes have been associated with poor prognosis various malignancies. However, abnormal expression their diagnostic prognostic value sarcomas (SARC) remain unclear. Methods: Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), Kaplan-Meier Plotter, Cancer cell line...
Abstract Breast cancer is one of the most common types which described as leading cause death in women. After competitive endogenous RNA (ceRNA) hypothesis was proposed, this triple regulatory network has been observed various cancers, and increasing evidences reveal that ceRNA plays a significant role migration, invasion, proliferation cells. In current study, our target to construct CD24-associated network, further identify key prognostic biomarkers breast cancer. Using transcriptom...
Flavin mononucleotide (FMN) is a ubiquitous blue-light pigment due to its ability drive one- and two-electron transfer reactions. In both light-oxygen-voltage (LOV) domains of phototropin from the green algae Chlamydomonas reinhardtii, FMN noncovalently bound. LOV1 cysteine-to-serine mutant (C57S), light-induced electron nearby tryptophan occurs, transient spin-correlated radical pair (SCRP) formed. Within this photocycle, nuclear hyperpolarization created by solid-state photochemically...
The Cover Feature shows the direct enzymatic degradation of post-consumer PET packaging by a novel metagenomic polyester hydrolase. monomers can be recovered and used for production virgin PET, thereby achieving closed-loop plastic recycling process with low carbon footprint. More information found in Full Paper C. Sonnendecker et al.
Abstract Background: Soft tissue sarcoma is among the most challenging malignancies for clinicians and associated with poor prognosis. Former studies have revealed that cancer-associated fibroblasts (CAFs) are involved in tumor development, migration, invasion metastasis.Methods: DEGs WGCNA were employed to obtain significant CAFs-related genes constructing signature. Somatic mutation, immune status, checkpoints chemotherapy sensitivity applied define characteristics of identified...