A5062758926- Kidney Stones and Urolithiasis Treatments
- Nutrition and Health in Aging
- Metabolomics and Mass Spectrometry Studies
- Pediatric Urology and Nephrology Studies
- Frailty in Older Adults
- Urinary Tract Infections Management
- Vitamin D Research Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Graphene and Nanomaterials Applications
- Metabolism and Genetic Disorders
- Nanocluster Synthesis and Applications
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- Body Composition Measurement Techniques
- Parkinson's Disease Mechanisms and Treatments
- Carbon and Quantum Dots Applications
- Bone health and osteoporosis research
- Cancer, Hypoxia, and Metabolism
- GDF15 and Related Biomarkers
- Parathyroid Disorders and Treatments
- Extracellular vesicles in disease
- Urinary Bladder and Prostate Research
- Protein Tyrosine Phosphatases
- Therapeutic Uses of Natural Elements
- Pelvic floor disorders treatments
Agostino Gemelli University Polyclinic
2016-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2023
Università Cattolica del Sacro Cuore
2014-2022
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2018
University of Florida
2018
Sapienza University of Rome
2018
Physical frailty and sarcopenia (PF&S) share multisystem derangements, including variations in circulating amino acids chronic low-grade inflammation. Gut microbiota balances inflammatory responses several conditions according to nutritional status. Therefore, an altered gut-muscle crosstalk has been hypothesized PF&S. We analyzed the gut microbial taxa, systemic inflammation, metabolic characteristics of older adults with without An innovative multi-marker analytical approach was applied...
Physical frailty and sarcopenia (PF&S) are hallmarks of aging that share a common pathogenic background. Perturbations in protein/amino acid metabolism may play role the development PF&S. In this initial report, 68 community-dwellers aged 70 years older, 38 with PF&S 30 non-sarcopenic, non-frail controls (nonPF&S), were enrolled as part “BIOmarkers associated Sarcopenia EldeRly pErsons” (BIOSPHERE) study. A panel 37 serum amino acids derivatives was assayed by UPLC-MS....
Recent evidence has shown that graphene quantum dots (GQDs) are capable of crossing the blood–brain barrier, barrier reduces cancer therapy efficacy. Here, we tested three alternative GQDs’ surface chemistries on two neural lineages (glioblastoma cells and mouse cortical neurons). We showed chemistry modulates biocompatibility. When used in combination with chemotherapeutic drug doxorubicin, GDQs exerted a synergistic effect tumor cells, but not neurons. This appears to be mediated by...
Physical frailty and sarcopenia (PF&S) is a prototypical geriatric condition characterized by reduced physical function low muscle mass. The multifaceted pathophysiology of this recapitulates all hallmarks aging making the identification specific biomarkers challenging. In present study, we explored relationship among three processes that are thought to be involved in PF&S (i.e., systemic inflammation, amino acid dysmetabolism, mitochondrial dysfunction). We took advantage well-characterized...
Glioblastoma is the most aggressive and lethal brain cancer. Current treatments involve surgical resection, radiotherapy chemotherapy. However, life expectancy of patients with this disease remains short chemotherapy leads to severe adverse effects. Furthermore, presence blood–brain barrier (BBB) makes it difficult for drugs effectively reach brain. A promising strategy lies in use graphene quantum dots (GQDs), which are light-responsive nanoparticles that have shown capability crossing BBB....
Diabetes and frailty are highly prevalent conditions that impact the health status of older adults. Perturbations in protein/amino acid metabolism associated with both functional impairment type 2 diabetes mellitus (T2DM). In present study, we compared concentrations a panel circulating 37 amino acids derivatives between frail/pre-frail adults T2DM robust non-diabetic controls. Sixty-six functionally impaired persons aged 70+ 30 age sex-matched controls were included analysis. We applied...
Background and Purpose Amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the degeneration of upper lower motor neurons, progressive wasting paralysis voluntary muscles is currently incurable. Although considered to be pure neuron disease, increasing evidence indicates that sole protection neurons single targeted drug not sufficient improve pathological phenotype. We therefore evaluated therapeutic potential multi‐target used treatment coronary artery...
Background. Kidney stones are a common illness with multifactorial etiopathogenesis. The determination of crystalline and molecular composition the quantification all stone components important to establish etiology disease but it is often laborious obtain using chemical method. aim this paper compare spot test FT-IR spectroscopy, for possible introduction in our laboratory. Methods. We analyzed 48 calculi Urinary Calculi Analysis kit accordance manufacturer’s instructions. same samples were...
Serum amino acid (AA) profiles represent a valuable tool in the metabolic assessment of cancer patients; still, information on AA pattern head and neck (HNC) patients is insufficient. The aim study was to assess whether serum levels were associated with stage neoplastic disease prognosis primary HNC patients.Two hundred two included study. Thirty-one AAs derivatives measured through an ultraperformance liquid chromatography-mass spectrometry (UPLC-MS). association between concentrations...
An altered amino acid metabolism has been described in frail older adults which may contribute to muscle loss and functional decline associated with frailty. In the present investigation, we compared circulating profiles of physical frailty sarcopenia (PF&S, n = 94), frail/pre-frail type 2 diabetes mellitus (F-T2DM, 66), robust non-diabetic controls (n 40). Partial least squares discriminant analysis (PLS-DA) models were built define signatures different phenotypes. PLS-DA allowed correct...
Introduction: Tacrolimus (TAC) stands out as one of the most widely utilized immu-nosuppressive drugs in prevention rejection following solid organ transplantation. In case liver transplantation (LTx), biochemical pathway for drug metabolism and excretion is an attribute transplanted organ, primary focus immunological functional moni-toring. Consequently, different reduced hepatic functions every patient may lead to di-minished TAC catabolism excretion, resulting a biliary concentration...
Global pandemic management represents a serious issue for health systems. In some cases, repurposing of existing medications might help find compounds that have the unexpected potential to combat microorganisms. same way, changing cell–drug interaction by nanotechnology could represent an innovative strategy fight infectious diseases. Tuberculosis (TB) remains one most alarming worldwide diseases and there is urgent need new drugs treatments, particularly emergence spread drug-resistant...
Physical frailty and sarcopenia (PF&S) are hallmarks of aging that share a common pathogenic background. Perturbations in protein/amino acid metabolism may play role the development PF&S. In this preliminary study, 68 community-dwellers aged 70 years older, 38 with PF&S 30 non-sarcopenic, non-frail controls (nonPF&S), were enrolled. A panel 37 serum amino acids derivatives was assayed by UPLC-MS. Partial Least Squares Discriminant Analysis (PLS-DA) used to...
Determination of urinary lactulose/mannitol is one the most used tests to evaluate intestinal barrier function. High-performance liquid chromatography (HPLC) separation with electrospray ionization tandem mass spectrometry guarantees high levels selectivity and reproducibility. In this paper we report an upgrade previous published method, introducing more reliable internal standards ultra-performance ethylene bridged hybrid amide columns. The provided efficient chromatographic two sugars in...
Aim . Lactulose/mannitol ratio is used to assess intestinal barrier function. of this work was develop a robust and rapid method for the analysis lactulose mannitol in urine by liquid chromatography coupled tandem mass spectrometry. has been measured pediatric patients suffering from irritable bowel syndrome. Methods Calibration curves raffinose, as internal standard, were prepared water : acetonitrile 20 80. Fifty μ L sample added 450 standard solution. The chromatographic separation...
Urolithiasis is the process of stone formation in urinary tract. Its etiology only partly known, and efficient therapeutic approaches are currently lacking. Metabolomics increasingly used biomarkers discovery for its ability to identify mediators relevant (patho)physiological processes. Amino acids may be involved kidney formation. The aim present study was investigate presence an amino acid signature former urine through a targeted metabolomic approach.A panel 35 derivatives assessed urines...
Glioblastoma multiforme (GBM) is the most aggressive brain cancer. We previously demonstrated effect of biocompatible surface functionalized graphene quantum dots (GQDs) on GBM cells as chemotherapy enhancers in combination with antitumor drug doxorubicin (Dox). However, traditional two-dimensional cultures could not represent a reliable model tumor behavior. In this work, we investigated carboxylated (COOH-GQDs), aminated (NH2-GQDs) and unfunctionalized GQDs three-dimensional neurospheres....