A5033981085- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Fire effects on ecosystems
- COVID-19 Clinical Research Studies
- Flood Risk Assessment and Management
- Inflammasome and immune disorders
- Heme Oxygenase-1 and Carbon Monoxide
- CAR-T cell therapy research
- Venous Thromboembolism Diagnosis and Management
- Long-Term Effects of COVID-19
- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Optimism, Hope, and Well-being
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Immune responses and vaccinations
University of Helsinki
2021-2024
Severe COVID-19 is characterized by extensive pulmonary complications, to which host immune responses are believed play a role. As the major arm of innate immunity, neutrophils one first cells recruited site infection where their excessive activation can contribute lung pathology. Low-density granulocytes (LDGs) circulating neutrophils, whose numbers increase in some autoimmune diseases and cancer, but poorly acute viral infections. Using flow cytometry, we detected significant LDGs blood...
The severity of COVID-19 is linked to excessive inflammation. Neutrophils represent a critical arm the innate immune response and are major mediators inflammation, but their role in pathophysiology remains poorly understood. We conducted transcriptomic profiling neutrophils obtained from patients with mild severe COVID-19, as well SARS-CoV-2 infected mice, comparison non-infected healthy controls. In addition, we investigated inflammasome formation potential mice upon infection....
Innate immune cells like monocytes patrol the vasculature and mucosal surfaces, recognize pathogens, rapidly redistribute to affected tissues cause inflammation by secretion of cytokines. We previously showed that are reduced in blood but accumulate airways patients with Puumala virus (PUUV) caused hemorrhagic fever renal syndrome (HFRS). However, dynamics monocyte infiltration kidneys during HFRS, its impact on disease severity currently unknown. Here, we examined longitudinal peripheral...
Old–world orthohantaviruses cause hemorrhagic fever with renal syndrome (HFRS), characterized by acute kidney injury (AKI) transient proteinuria. It seems plausible that proteinuria during HFRS is mediated the disruption of glomerular filtration barrier (GFB) due to vascular leakage, a hallmark orthohantavirus–caused diseases. However, direct infection endothelial cells does not result in increased permeability, and alternative explanations for leakage diminished GFB function are necessary....
Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome (PUUV-HFRS) is characterized by strong neutrophil activation. Neutrophils are the most abundant immune cell type in circulation and specially equipped to rapidly respond infections. They more heterogenous than previously appreciated, specific subsets recently implicated inflammation immunosuppression. Furthermore, neutrophils can be divided based on their density either low-density granulocytes (LDGs) or "normal density"...
In humans, orthohantaviruses can cause hemorrhagic fever with renal syndrome (HFRS) or hantavirus pulmonary (HPS). An earlier study reported that acute Andes virus HPS caused a massive and transient elevation in the number of circulating plasmablasts specificity towards both viral host antigens suggestive polyclonal B cell activation. Immunoglobulins (Igs), produced by different populations, comprise heavy light chains; however, certain amount free chains (FLCs) is constantly present serum....
Old World Orthohantaviruses cause hemorrhagic fever with renal syndrome (HFRS) characterized by increased vascular permeability and acute kidney injury (AKI). Despite the systemic nature of disease, virus enters humans through inhalation therefore initially encounters immunoglobulin class A (IgA) dominated mucosal immune system. Herein, we IgA responses their potential relationship to activation examining blood samples obtained from patients hospitalized due Puumala orthohantavirus...
Though cryopreservation of cell fractions is widely used in flow cytometry studies, whole blood more challenging due to the presence erythrocytes and effects fixatives commonly for preservation. Here, we evaluated compared head-to-head performance four commercial kits; (1) Cytodelics, (2) Stable-Lyse V2 Stable-Store (SLSS-V2), (3) Proteomic stabilizer (PROT-1), (4) Transfix. We found that PROT-1, Transfix, Cytodelics maintained distribution major leukocyte subsets-granulocytes, T cells,...
Abstract The severity of COVID-19 is linked to excessive inflammation. Neutrophils represent a critical arm the innate immune response and are major mediators inflammation, but their role in pathophysiology remains poorly understood. We conducted transcriptomic profiling neutrophils obtained from patients with mild severe COVID-19, as well SARS-CoV-2 infected mice, comparison non-infected healthy controls. In addition, we investigated inflammasome formation potential mice upon infection....
Abstract Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome (PUUV-HFRS) is characterized by strong neutrophil activation. Neutrophils are the most abundant immune cell type in circulation and specially equipped to rapidly respond infections. They more heterogenous than previously appreciated, specific subsets recently implicated inflammation immunosuppression. Furthermore, neutrophils can be divided based on their density either low-density granulocytes (LDGs) or “normal...
Abstract Old World Orthohantaviruses cause hemorhagic fever with renal syndrome (HFRS) characterized by increased vascular permeability and acute kidney injury (AKI). Despite the systemic nature of disease, virus enters humans through inhalation therefore initially encounters immunoglobulin class A (IgA) dominated mucosal immune system. Herein, we IgA responses their potential relationship to activation examining blood samples obtained from patients hospitalized due Puumala orthohantavirus...
Abstract Vascular leakage characterizes Puumala orthohantavirus (PUUV)-caused hemorrhagic fever with renal syndrome (HFRS). Disruption in the endothelial glycocalyx layer, which protects blood vessels from increased vascular into tissues, may contribute to disease severity during acute PUUV-HFRS. Recent evidence suggests that heparanase (HPSE) activity could play a role degradation. Additionally, dynamic changes monocyte populations, especially decrease endothelium “patrolling” CD14 + CD16...