A5081982394- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- Immune cells in cancer
- Autophagy in Disease and Therapy
- Cancer Research and Treatments
- Virus-based gene therapy research
- Icing and De-icing Technologies
- Inflammatory Biomarkers in Disease Prognosis
- interferon and immune responses
- Hepatitis B Virus Studies
- Pancreatic function and diabetes
- CAR-T cell therapy research
- Phosphodiesterase function and regulation
- Cholinesterase and Neurodegenerative Diseases
- Phagocytosis and Immune Regulation
- Nanoplatforms for cancer theranostics
- Sexual function and dysfunction studies
- vaccines and immunoinformatics approaches
- Ultrasound and Hyperthermia Applications
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- Metabolism, Diabetes, and Cancer
- Lymphoma Diagnosis and Treatment
Southeast University
2014-2021
Shanghai Cell Therapy Research Institute
2020
Zhongda Hospital Southeast University
2016-2019
A better understanding of the immune profile non-small cell lung cancer (NSCLC) and immunomodulatory impact chemotherapy is essential to develop current therapeutic approaches. Herein, we collected peripheral blood from 20 healthy donors 50 patients with advanced NSCLC, before after chemotherapy, followed by phenotypic analysis lymphocyte subsets assessment correlation between their post-chemotherapy levels progression-free survival (PFS). Results showed that, CD8+ lymphocytes, PD-1+CD4+,...
Pancreatic cancer is one of the most lethal malignancies all types due to lack early symptoms and its resistance conventional therapy. In our previous study, we have shown that v‑ets erythroblastosis virus E26 oncogene homolog‑1 (ETS‑1) promote cell migration invasion in pancreatic cells. However, function ETS‑1 regulation glycolysis autophagy during progression has not been defined yet. this sought identify potential role for silencing reducing expression glucose transporter‑1 (GLUT‑1)...
Our previous studies have confirmed that tumor cell-released autophagosomes (TRAP) could induce the differentiation of B cells into IL-10+ regulatory (Bregs) with suppressive activities on T lymphocytes. However, mechanism TRAP-mediated immune suppression is still largely unclear. Herein, we sought to assess immunomodulatory effect TRAPs human neutrophils, a major cell type infiltrates tissues. We found enriched from malignant effusions or ascites cancer patients and lines were rapidly...
A simple and effective strategy was developed to enrich ubiquitinated proteins (UPs) from cancer cell lysate using the α-Al2O3 nanoparticles covalently linked with ubiquitin binding protein (Vx3) (denoted as α-Al2O3–Vx3) via a chemical linker. The functionalized α-Al2O3–Vx3 showed long-term stability high efficiency for enrichment of UPs lysates. Flow cytometry analysis results indicated dendritic cells (DCs) could more effectively phagocytize α-Al2O3–Vx3-UPs than physical mixture Vx3-UPs...
We previously reported that enriched ubiquitinated proteins (UPs) from tumor cells have the potential to be used as immunotherapy vaccine against cancer. Here we UPs epirubicin (EPB)-induced multi-drug-resistant cancer stem-like breast cell line (4T1/EPB) and tested efficacy of α-Al 2 O 3 -UPs-4T1/EPB (short for UPs-4T1/EPB) therapeutic alone in combination with stimulator interferon genes (STING) agonist mice drug-resistant metastatic Vaccination UPs-4T1/EPB exerted profound anti-tumor...
Our previous studies have demonstrated that autophagosome-enriched vaccine (named DRibbles: DRiPs-containing blebs) induce a potent anti-tumor efficacy in different murine tumor models, which DRibble-containing ubiquitinated proteins are efficient tumor-specific antigen source for the cross-presentation after being loaded onto dendritic cells. In this study, we sought to detect whether enriched from cells could be used directly as novel cancer vaccine. The ubiquitin binding protein Vx3(A7)...
Aim: Severe cardiac toxicity of doxorubicin and an immunosuppressive tumor micro-environment become main obstacles for the effective treatment B-cell lymphoma. In this research, rituximab-conjugated doxorubicin-loaded microbubbles (RDMs) were designed exploring a combination approach targeted with ultrasound (US) irradiation PD-1 inhibitor to overcome mentioned above. Methods: vivo studies performed on SU-DHL-4 cell-grafted mice ex CD20+ human cells T cells. Results: A greater therapeutic...
The success of peptide-based dendritic cell (DC) cancer vaccines mainly depends on the utilized peptides and selection an appropriate adjuvant. Herein, we aimed to evoke a broad immune response against multiple epitopes concurrently in presence immunoadjuvant. Three synthetic HLA-A<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mo>∗</mml:mo></mml:math>0201-restricted were separately linked with HMGB1-derived peptide (SAFFLFCSE, denoted as HB 100-108 ) immunoadjuvant via...
Phosphodiesterase 5 (PDE5) is a catalytic enzyme for degradation of cyclic guanosine monophosphate (cGMP) in human smooth muscle cells. Inhibition this by certain chemicals including sildenafil, vardenafil and tadalafil, considered breakthrough treatment erectile dysfunction. Unfortunately, these drugs are associated with several side effects. In study, another strategy adopted the inhibition PDE5 using monoclonal antibody (Mab) against enzyme. The Mab was prepared immunizing mouse, taking...
e24125 Background: Recently, there has been a growing interest in lung cancer immunotherapy due to its preliminary results achieving meaningful treatments responses. We conducted this research for more understanding the immunophenotyping profile of peripheral blood from patients with advanced non-small cell (NSCLC) after chemotherapy and correlation patients’ survival. Methods: Peripheral samples were collected 15 healthy donors 35 NSCLC two cycles chemotherapy. Phenotypic analysis...