Background: Oxidative stress from elevated reactive oxygen species (ROS) has been reported to induce cell apoptosis and may provide a means target cancer cells. Celastrol is natural bioactive compound that was recently shown increase ROS levels cause in However, the underlying mechanism for this cytotoxic action remains unclear direct molecular targets of have not identified. Methods: Proteome microarray, surface plasmon resonance, isothermal titration calorimetry simulation were used...

Background Trichinellosis is a serious zoonositc parasitosis worldwide. Because its clinical manifestations aren't specific, the diagnosis of trichinellosis not easy to be made. Trichinella spiralis muscle larva (ML) excretory–secretory (ES) antigens are most widely applied diagnostic for human trichinellosis, but major drawback ES assaying anti-Trichinella antibodies false negative in early infection period. The aim this study was characterize T. putative serine protease (TsSP) and...

Background Gut epithelium is the first natural barrier against Trichinella spiralis larval invasion, but mechanism by which penetration of gut not completely elucidated. Previous studies showed that proteases secreted T . intestinal infective larvae (IIL) degraded tight junctions (TJs) proteins and mediated invasion. A new serine proteinase (TsSPc) was identified in IIL surface ES proteins, rTsSPc bound to epithelial cell (IECs) promoted invasion IECs. The aim this study characterize...

The aim of this study was to the molecular characteristics Trichinella spiralis galectin (Tsgal) and interactions between Tsgal host's intestinal epithelial cells (IECs). functional domain cloned expressed in an E. coli system. 97.1% identity T. nativa 20.8% galectin-8 humans. Conserved analysis revealed that belongs TR-type has two carbon recognized domain. rTsgal with 29.1 kDa could be by spiralis-infected mice at 42 days post-infection (dpi). transcription expression gene detected RT-PCR...

In our previous work, a Trichinella spiralis putative serine protease (TsSP) was identified from ES products of T. intestinal infective larvae (IIL) and adult worms (AW) by immunoproteomics: it highly expressed in IIL compared with muscle (ML). this study, the TsSP biological characteristics larval invasion growth were its potential as vaccine target against infection investigated. Expression at various developmental phases (newborn larvae, ML, IIL, AW) detected qPCR, immunofluorescent test...

Trichinella spiralis serine protease inhibitor (TsSPI) was identified in ES proteins of adult worms (AW), the TsSPI gene highly expressed at enteral stage (AW and newborn larvae), distributed mainly cuticle stichosome this nematode. Vaccination mice with rTsSPI exhibited a 62.2% reduction intestinal AW 57.25% muscle larvae after larval challenge. The aim study to investigate biological characteristics its roles process T. invasion host's epithelium cells (IECs). inhibition on trypsin...

Nudix hydrolases (Nd) is a widespread superfamily, which found in all classes of organism, hydrolyse wide range organic pyrophosphates and has ‘housecleaning’ function. The previous study showed that Trichinella spiralis Nd (TsNd) bound to intestinal epithelial cells (IECs), the vaccination mice with T7 phage-displayed TsNd polypeptides produced protective immunity. aim this was clone, express identify full-length investigate its immune protection against T. infection. cDNA sequence gene...

Trichinella spiralis is a zoonotic tissue-dwelling parasitic nematode that infects humans and other mammals. Its surface proteins are recognized as antigenic in many infected hosts, being directly exposed to the host's immune system main target antigens induce responses. The larval may also interact with intestinal epithelial cells play an important role invasion development process of T. spiralis. purpose this study was analyze characterize muscle larvae by two-dimensional gel...

Background Sparganosis is a neglected but important food-borne parasitic zoonosis. Clinical diagnosis of sparganosis difficult because there are no specific manifestations. ELISA using plerocercoid crude or excretory–secretory (ES) antigens has high sensitivity cross-reactions with other helminthiases. The aim this study was to characterize Spirometra erinaceieuropaei cysteine protease (SeCP) and evaluate its potential application for serodiagnosis sparganosis. Methodology/Principal Findings...

Previous study showed that Trichinella spiralis proteasome subunit beta type-7 (Tspst) gene is an up-regulated in intestinal infective larvae (IIL) compared to muscle (ML), which was screened by using suppression subtractive hybridization (SSH) and confirmed real-time PCR. Tspst may be related the larval invasion of epithelial cells (IECs). The aim this identify investigate its immune protection against T. infection.The encoding a 29 kDa protein from cloned, recombinant (rTspst) produced...

Trichinella spiralis is an important foodborne zoonotic parasite and it necessary to develop a vaccine in order interrupt transmission from animals humans. A 31 kDa protein T. (Ts31) antigen targeted by protective antibodies, Ts31 contains domain of trypsin-like serine protease that might have the function protease. The purpose this study was investigate molecular characteristics its induced immune protection. Expression localization various phases were investigated using qPCR...

Deoxyribonuclease II (DNase II) is a widespread endonuclease which can degrade the DNA. Trichinella spiralis adult-specific DNase II-1 (TsDNase II-1)/DNase II-7 II-7) were identified in excretory–secretory (ES) or surface proteins of adult worm (AW) and intestinal infective larvae (IIL) using immunoproteomics with early infection sera. The aim this study was to characterize two T. investigate their potential for vaccine candidate target molecules. cDNA sequences from 3 d AW cloned expressed....

Serine protease inhibitors (SPI) are a superfamily of the proteins able to suppress serine activity, and may exert major biological function in complement activation, inflammation, fibrinolysis. A SPI was identified from Trichinella spiralis adult worms (AW) by immunoproteomics with early infection sera. The aim this study investigate protective immune elicited TsSPI. complete TsSPI cDNA sequence cloned into pQE-80 L then expressed Escherichia coli BL21. rTsSPI purified its antigenicity...

Trichinella spiralis muscle larval (ML) excretion/secretion (ES) antigen is the most widely used diagnostic of trichinellosis, but preparation ES requires collecting worms from infected animals, and detection specific IgG against ML may result in a false negative at early stage infection. The aim study was to characterize T. elastase-1 (TsEla) evaluate its potential as for trichinellosis. complete cDNA sequences TsEla gene were cloned expressed, recombinant (rTsEla) purified. transcription...

Abstract The aim of this study was to investigate the biological characteristics and functions a Trichinella spiralis serine proteinase (TsSerp) during larval invasion development in host. full-length TsSerp cDNA sequence cloned expressed Escherichia coli BL21. results RT-PCR, IFA western blotting analyses showed that secretory protein highly at T. intestinal infective larva muscle stages primarily located cuticle, stichosome intrauterine embryos parasite. rTsSerp promoted epithelial cells...

Artificial digestion method is widely used for the detection of Trichinella larvae (mainly mature larvae, e.g., encapsulated in Trichinella) meat. The previous studies demonstrated that spiralis pre-encapsulated (PEL) at 14-18 days postinfection (dpi) had infectivity to new hosts. However, our knowledge, there no report on methods PEL purpose this study was compare efficiency artificial and Baermann's T. meat, test factors affecting sensitivity two methods. Forty-five male Kunming mice were...

Staphylinoidea (Insecta: Coleoptera) is one of the most species-rich groups in animals, but its huge diversity can hardly be explained by popular hypothesis (co-radiation with angiosperms) that applies to phytophagous beetles. We estimated evolutionary mode staphylinoid beetles and investigated relationship between palaeoclimate change, thus factors underlying current biodiversity pattern Our results demonstrate originated at around Triassic-Jurassic bound higher level clades underwent rapid...