A5016066988- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- Malaria Research and Control
- Virology and Viral Diseases
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Insect symbiosis and bacterial influences
- Respiratory viral infections research
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- Infectious Encephalopathies and Encephalitis
- Whipple's Disease and Interleukins
- COVID-19 epidemiological studies
- Vaccine Coverage and Hesitancy
- Animal Disease Management and Epidemiology
- Cytomegalovirus and herpesvirus research
- Long-Term Effects of COVID-19
- Animal Virus Infections Studies
- Travel-related health issues
- Dengue and Mosquito Control Research
- Insect and Pesticide Research
- T-cell and Retrovirus Studies
- Rabies epidemiology and control
Ministry of Public Health
2015-2024
Department of Disease Control
2015-2024
Armed Forces Research Institute of Medical Science
2015-2021
Keelung Chang Gung Memorial Hospital
2021
Thai Red Cross Society
2021
Chulalongkorn University
2021
University of Toronto
2015
Zika virus (ZIKV) is an emerging mosquito-borne pathogen with reported cases in Africa, Asia, and large outbreaks the Pacific. No autochthonous ZIKV infections have been confirmed Thailand. However, there several travelers returning from Here we report seven of acute infection Thai residents across country by molecular or serological testing including sequence data. These endemic cases, combined previous reports travelers, provide evidence that widespread throughout
We report two cases of coronavirus disease 2019 (COVID-19) in travellers from Wuhan, China to Thailand. Both were independent introductions on separate flights, discovered with thermoscanners and confirmed RT-PCR genome sequencing. do not seem directly linked the Huanan Seafood Market Hubei but viral genomes are identical four other sequences suggesting early spread within city already first week January.
Abstract In the age of a pandemic, such as ongoing one caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), world faces limited supply tests, personal protective equipment, and factories chains are struggling to meet growing demands. This study aimed evaluate efficacy specimen pooling for testing SARS‐CoV‐2 virus, determine whether costs resource savings could be achieved without impacting sensitivity testing. Ten previously tested nasopharyngeal throat swab specimens...
Background Disease incidence data are needed to guide decision-making for public health interventions. Although dengue is a reportable disease in Thailand and Cambodia, the degree that reported underrecognizes true burden unknown. We utilized calculated from laboratory-confirmed outpatient inpatient cases prospective cohort studies estimate magnitude of underrecognition establish more accurate estimates these countries. Methods Findings Cohort were conducted among children aged <15 years by...
Dengue virus has traditionally caused substantial morbidity and mortality among children less than 15 years of age in Southeast Asia. Over the last 2 decades, a significant increase mean cases been reported, once pediatric disease now causes burden adult population. An age-stratified serological study (n = 1,736) was conducted 2010 schoolchildren Mueang Rayong district Thailand, where similar had 1980/1981. Serotype-specific forces infection (λ(t)) basic reproductive numbers (R0) dengue were...
Zika virus (ZIKV) is a mosquito-borne member of the genus Flavivirus that has emerged since 2007 to cause outbreaks in Africa, Asia, Oceania, and most recently, Americas. Here, we used an isolate history as well genetic phylogenetic analyses characterize three low-passage isolates representing African (ArD 41525) Asian (CPC-0740, SV0127-14) lineages investigate potential phenotypic differences vitro vivo. The displayed large plaque phenotype (∼3-4 mm) on Vero HEK-293 cells, whereas either...
Zika virus (ZIKV) infection is an emerging and re-emerging arbovirus disease that transmitted to humans through the bite of infected mosquitoes. ZIKV infections were first described in Thailand 1954 from sera indigenous residents several travelers returning 2014. However, reported cases have been increasing since 2015 2016, epidemiological information about vectors unclear. We investigated molecular epidemiology genetic diversity mosquitoes collected different geographic regions experiencing...
Several mosquito species have been described as vectors for the Zika virus (ZIKV), such those in Aedes, Anopheles, Mansonia and Culex genera. Our previous survey studies were found ZIKV RNA positive both male, female larvae of quinquefasciatus Say Aedes aegypti (L.) mosquitoes collected from active infected patients' homes Thailand. Therefore, aims this study to investigate whether could be vertically transmitted Cx. quinquefasciatus, Ae. albopictus. Laboratory field colonies these...
BackgroundEnterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are the major causative agents of hand, foot mouth disease (HFMD) worldwide, particularly in Asia-Pacific region. Several strains have emerged, circulated, faded out over time recent decades. This study investigated EV-A71 CV-A16 circulating replacement genotypes/subgenotypes Thailand during years 2000–2017.MethodsThe complete VP1 regions 92 enteroviruses obtained from 90 HFMD patients, one asymptomatic adult contact case,...
We conducted molecular characterization, demonstrated the geographical distribution of Zika virus (ZIKV) circulating worldwide from 1947 to 2022 and explored potential genetic recombination site in Thailand ZIKV genomes.We constructed phylogenetic trees based on coding sequences (CDS) determined representative viruses by relationship timeline. among between other flaviviruses using similarity plot bootscan analyzes, together with phylogeny encompassing CDS eight subgenomic regions.The...
Emerging coronaviruses (CoVs) are understood to cause critical human and domestic animal diseases; the spillover from wildlife reservoirs can result in mild severe respiratory illness humans animals spread more readily these naïve hosts. A low-cost CoV molecular method that detect a variety of CoVs humans, animals, environmental specimens is an initial step ensure early identification known new viruses. We examine collection 50 human, 46 wastewater, 28 bat, 17 avian archived using 3...
ABSTRACT Here, we present the complete genome sequences of two Zika virus (ZIKV) strains, virus/ Homo sapiens -tc/THA/2014/SV0127-14 and H. -tc/PHL/2012/CPC-0740, isolated from blood patients collected in Thailand, 2014, Philippines, 2012, respectively. Sequencing phylogenetic analysis showed that both strains belong to Asian lineage.
Chikungunya virus (CHIKV) is a mosquito-borne belonging to the genus Alphavirus. The transmitted humans by bite of infected female Aedes mosquitoes, primarily aegypti. CHIKV infection spreading worldwide, and it periodically sparks new outbreaks. There are no specific drugs or effective vaccines against CHIKV. interruption pathogen transmission mosquito control provides only approach infection. Many studies have shown that can be among Ae. aegypti through vertical transmission. previous...
Hand, foot, and mouth disease (HFMD) caused by enteroviruses remains a public health threat, particularly in the Asia-Pacific region during past two decades. Moreover, introduction of multiple subgenotypes emergence recombinant viruses is epidemiological importance. Based on either full genome or VP1 sequences, 32 (30 from HFMD patients, 1 an encephalitic patient, asymptomatic contact case) isolated Thailand between 2006 2014 were identified as 25 enterovirus 71 (EV71) isolates (comprising...
Wastewater surveillance is considered a promising approach for COVID-19 in communities. In this study, we collected wastewater samples between November 2020 and February 2022 from twenty-three sites the Bangkok Metropolitan Region to detect presence of SARS-CoV-2 its variants comparison standard clinical sampling. A total 215 were tested RNA by real-time PCR with three targeted genes (N, E, ORF1ab); 102 positive (42.5%). The determined multiplex MassARRAY assay distinguish four variants,...
Thailand reported the first Middle East respiratory syndrome (MERS) case on 18 June 2015 (day 4) in an Omani patient with heart condition who was diagnosed pneumonia hospital admission 15 1). Two false negative RT-PCR upper tract samples days 2 and 3 led to a 48-hour diagnosis delay decision transfer out of pressure unit (NPU). Subsequent examination sputum later day confirmed MERS coronavirus (MERS-CoV) infection. The immediately moved back into NPU then transferred Bamrasnaradura...
Abstract The rapid emergence of SARS-CoV-2 variants with high severity and transmutability adds further urgency for multiplex molecular testing to identify the variants. A nucleotide matrix-assisted laser-desorption-ionization time-of-flight mass spectrophotometry (MALDI-TOF MS)-based assay was developed (called point mutation array, PMA) four major concern (VOCs) including Alpha, Beta, Delta, Omicron (namely PMA-ABDO) differentiate subvariant PMA-Omicron). PMA-ABDO PMA-Omicron consist 24 28...
In vitro determination of severe acute respiratory syndrome coronavirus 2 neutralizing antibodies induced in serum samples from recipients the CoronaVac vaccine showed a short protection period against original virus strain and limited variants concern. These data provide support for boosters, especially concern circulate.
Abstract Background Nipah virus (NiV) infection causes encephalitis and has > 75% mortality rate, making it a WHO priority pathogen due to its pandemic potential. There have been NiV outbreak(s) in Malaysia, India, Bangladesh, southern Philippines. naturally circulates among fruit bats of the genus Pteropus detected widely across Southeast South Asia. Both Malaysian Bangladeshi strains found Thailand. This study summarizes 20 years pre-emptive One Health surveillance Thailand, including...
In early January 2020, Thailand became the first country where a coronavirus disease 2019 (COVID-19) patient was identified outside China. this study, 23 whole genomes of severe acute respiratory syndrome 2 (SARS-CoV-2) from patients who were hospitalized to March 2020 analyzed, along with their travel histories. Six lineages including A, A.6, B, B.1, B.1.8, and B.58, among which lineage A.6 dominant. Seven China traveled in February. Five them infected B virus, other two cases different A...
Here, we describe the development of in-house anti-Zika virus (ZIKV) IgM antibody capture ELISA (in-house ZIKV ELISA) for detection and diagnosis acute infections. We compared assay performance against two commercial kits, Euroimmun InBios 2.0 ELISA. tested assays' ability to detect anti-ZIKV using a well-defined serum sample panel. This panel included 80 negative samples (20 negative, 20 found be primary dengue [DENV][ infections, secondary DENV Japanese encephalitis [JEV] infections) 67...
The emergence of Omicron as the fifth variant concern within SARS-CoV-2 pandemic in late 2021, characterized by its rapid transmission and distinct spike gene mutations, underscored pressing need for cost-effective efficient methods to detect viral variants, especially given their evolving nature. This study sought address this assessing effectiveness two classification platforms based on RT-PCR mass spectrometry. primary aim was differentiate between Delta, BA.1, BA.2 variants using 618...
In resource-limited countries, early detection of novel pathogens is often challenging, due to financial and technical constraints. This study reports the efficacy family-wide polymerase chain reaction (PCR) in screening, detecting identifying initial cases SARS-CoV-2 Thailand. Respiratory secretions were collected from suspected individuals travelling Wuhan, China Thailand at beginning January 2020. Family-wide PCR assays yielded positive results for coronavirus one traveller within 12...
Zika virus (ZIKV) continues to affect certain parts of the World. Here we report a case that supports breastfeeding regardless mother ZIKV status by providing clinical and virological studies.
We determined the levels of neutralizing antibodies against SARS-CoV-2 ancestral strain, Delta and Omicron variants concern (VOCs), in 125 healthcare workers who received CoronaVac as their primary vaccination later either a single ChAdOx1 or combi-nation two consecutive boosters using doses BNT162b2 second boosters, respectively, BNT162b2. The titers 12 weeks after were inadequate to neutralize all strains. After booster, neutralization at Day 30 varied significantly, with only small...