A5065095901- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Botulinum Toxin and Related Neurological Disorders
- Neurological disorders and treatments
- Peroxisome Proliferator-Activated Receptors
- Metabolism, Diabetes, and Cancer
- Hereditary Neurological Disorders
- Hepatitis B Virus Studies
- Mosquito-borne diseases and control
- Nuclear Receptors and Signaling
- RNA Interference and Gene Delivery
- Cancer Treatment and Pharmacology
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Retinoids in leukemia and cellular processes
- Biotin and Related Studies
- Viral gastroenteritis research and epidemiology
- 14-3-3 protein interactions
- Adipose Tissue and Metabolism
- Insect symbiosis and bacterial influences
- Cholesterol and Lipid Metabolism
- Antibiotic Resistance in Bacteria
- Protein Kinase Regulation and GTPase Signaling
- Histone Deacetylase Inhibitors Research
- vaccines and immunoinformatics approaches
United States Army Medical Research Institute of Infectious Diseases
2012-2022
National Cancer Institute
2022
United States Army Medical Research and Development Command
2014-2015
Telemedicine & Advanced Technology Research Center
2014-2015
Jackson Foundation
2014-2015
Henry M. Jackson Foundation
2014-2015
The Geneva Foundation
2014
Pfizer (United States)
2006-2009
Duke University Hospital
1998-2003
Duke Medical Center
1998-2003
Mavrilimumab, a human monoclonal antibody targeting the alpha subunit of granulocyte-macrophage colony-stimulating factor receptor, was evaluated in phase 2 randomised, double-blind, placebo-controlled study to investigate efficacy and safety subjects with rheumatoid arthritis (RA).Subcutaneous mavrilimumab (10 mg, 30 50 or 100 mg) placebo administered every other week for 12 weeks on stable background methotrexate therapy. The primary endpoint proportion achieving ≥1.2 decrease from...
In C2C12 myoblasts, endogenous histone deacetylase HDAC4 shuttles between cytoplasmic and nuclear compartments, supporting the hypothesis that its subcellular localization is dynamically regulated. However, upon differentiation, this dynamic equilibrium disturbed we find accumulates in nuclei of myotubes, suggesting a positive role muscle differentiation. Consistent with notion regulation intracellular trafficking, reveal contains modular structure consisting C-terminal autonomous export...
Zika virus (ZIKV) is a mosquito-borne member of the genus Flavivirus that has emerged since 2007 to cause outbreaks in Africa, Asia, Oceania, and most recently, Americas. Here, we used an isolate history as well genetic phylogenetic analyses characterize three low-passage isolates representing African (ArD 41525) Asian (CPC-0740, SV0127-14) lineages investigate potential phenotypic differences vitro vivo. The displayed large plaque phenotype (∼3-4 mm) on Vero HEK-293 cells, whereas either...
The crystal structure of the adipocyte lipid-binding protein (ALBP) with coordinated fatty acid shows hydrophobic ligand bound within a water-filled central cavity its carboxyl group engaged in hydrogen bonding network involving, at least part, functional groups residues R126 and Y128. We produced mutant forms ALBP which altered these amino acids, expressed Escherichia coli as glutathione S-transferase (GST) fusion proteins, examined their ligand-binding properties using fluorescent acids...
Filoviridae currently includes five official and one proposed genera. Genus Ebolavirus established ebolavirus species for Bombali virus (BOMV), Bundibugyo (BDBV), Ebola (EBOV), Reston (RESTV), Sudan (SUDV) Taï Forest (TAFV), genus Marburgvirus a single Marburg (MARV) Ravn (RAVV). (EBOV) has emerged as significant public health concern since the 2013–2016 Virus Disease outbreak in Western Africa. Currently, there are no therapeutics approved need Ebola-specific remains gap. In search...
The keratinocyte lipid-binding protein (KLBP) has been identified on the basis of nucleotide sequence analysis its cloned cDNA as a new member intracellular (iLBP) multigene family. To characterize KLBP and determine ligand-binding properties, was subcloned into Escherichia coli, overexpressed purified to homogeneity by combination acid extraction, gel permeation, ion-exchange chromatographies. Purified exhibited high-affinity binding fluorescent hydrophobic probe...
Efficacious therapeutics for Ebola virus disease are in great demand. infections mediated by mucosal exposure, and aerosolization particular, present a novel challenge due to nontypical massive early infection of respiratory lymphoid tissues. We performed randomized blinded study compare outcomes from vehicle-treated remdesivir-treated rhesus monkeys lethal model resulting aerosolized exposure. Remdesivir treatment initiated 4 days after exposure was associated with significant survival...
Emerging infectious diseases like those caused by arboviruses such as Venezuelan equine encephalitis virus (VEEV) pose a serious threat to public health systems. Development of medical countermeasures against emerging are utmost importance. In this work, an acrylate and vinyl sulfone-based chemical series was investigated promising starting scaffolds VEEV inhibitors the cysteine protease domain VEEV's nonstructural protein 2 (nsP2). Primary screen dose response studies were performed...
Lectin-like oxidized LDL (ox-LDL) receptor-1 (LOX-1) is a type-II transmembrane protein that belongs to the C-type lectin family of molecules. LOX-1 acts as cell surface endocytosis receptor and mediates recognition internalization ox-LDL by vascular endothelial cells. Internalization results in number pro-atherogenic cellular responses implicated development progression atherosclerosis. In an effort elucidate functional domains responsible for binding receptor, series site-directed mutants...
The nuclear receptor peroxisome proliferator-activated α (PPARα) is recognized as the primary target of fibrate class hypolipidemic drugs and mediates lipid lowering in part by activating a transcriptional cascade that induces genes involved catabolism lipids. We report here characterization three novel PPARα agonists with therapeutic potential for treating dyslipidemia. These structurally related compounds display potent selective binding to human support robust recruitment coactivator...
A number of crystallographic studies the adipocyte lipid-binding protein have established that fatty acid-binding site is within an internalized water-filled cavity. The same also suggested existence a region physically distinct from which connects cavity with external solvent, hereafter referred to as portal. In effort examine portal region, we used site-directed mutagenesis introduce mutations V32D/F57H into murine ALBP cDNA. Mutant has been isolated, crystallized, and its stability...