A5038216980- Fungal Infections and Studies
- Lipid Membrane Structure and Behavior
- Peroxisome Proliferator-Activated Receptors
- Protein Structure and Dynamics
- Antifungal resistance and susceptibility
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- Plant Pathogens and Fungal Diseases
- Enzyme Structure and Function
- Receptor Mechanisms and Signaling
- Drug Transport and Resistance Mechanisms
- Carbohydrate Chemistry and Synthesis
- Scientific Computing and Data Management
- RNA and protein synthesis mechanisms
- Cholesterol and Lipid Metabolism
- Chemical Synthesis and Analysis
- Enzyme Catalysis and Immobilization
- Yeasts and Rust Fungi Studies
- Protein Interaction Studies and Fluorescence Analysis
- Plant Disease Resistance and Genetics
- Bioinformatics and Genomic Networks
- Parasitic Infections and Diagnostics
- Heat shock proteins research
- Protein Degradation and Inhibitors
- Galectins and Cancer Biology
University of Health Sciences and Pharmacy
2015-2019
King's College - Pennsylvania
2013
Commonwealth Medical College
2013
Rutgers, The State University of New Jersey
2006-2007
San Diego Supercomputer Center
2007
National Center for Biotechnology Information
2006
European Bioinformatics Institute
2006
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2006
University of Minnesota
1997-2006
Washington University in St. Louis
2003-2004
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The Worldwide Protein Data Bank (wwPDB; wwpdb.org) is the international collaboration that manages deposition, processing and distribution of PDB archive. online archive at ftp://ftp.wwpdb.org repository for coordinates related information more than 47 000 structures, including proteins, nucleic acids large macromolecular complexes have been determined using X-ray crystallography, NMR electron microscopy techniques. members wwPDB–RCSB (USA), MSD-EBI (Europe), PDBj (Japan) BMRB (USA)–have...
Cryptococcus neoformans (Cn) is a pathogenic yeast and the cause of cryptococcal meningitis. Prevalence disease between males females skewed, with having an increased incidence disease. Based on reported gender susceptibility differences to Cn in literature, we used clinical isolates from Botswanan HIV-infected patients test hypothesis that different environments exerted selective pressures Cn. When examined this data set, found men had significantly higher risk death despite CD4+ T...
The physiological consequences of molecular chaperone overproduction in Escherichia coli are presented. Constitutive DnaK from a multicopy plasmid containing large chromosomal fragments spanning the dnaK region resulted instability. Co-overproduction DnaJ with stabilized levels. To examine effects altered levels and more specific manner, an inducible expression system for dnaJ was constructed characterized. Differential rates synthesis were determined by quantitative Western blot...
Abstract Cryptococcus neoformans is an opportunistic fungal pathogen responsible for serious meningitis. Although many useful molecular tools have been developed its study, there are currently few inducible promoters available general use. To address this need, we constructed expression plasmids incorporating upstream elements of the C. copper transporter gene CTR4 , and tested them in serotypes A D. In response to deprivation, these mediate high‐level a reporter protein. This can be...
We have investigated a large set of interactions from the Helicobacter pylori protein interaction map previously identified by high-throughput yeast two-hybrid (htY2H)-based methods. This study had two aims: i) to validate htY2H as source protein-protein complexes for biochemical and structural studies H. interactome; ii) biochemically shown involve components type IV secretion systems. Thus, 17 involving 31 proteins fragments were studied, general strategy was designed produce...
UDP-glucose dehydrogenase catalyses the conversion of into UDP-GlcA, a critical precursor for glycan synthesis across evolution. We have cloned gene encoding this important enzyme from opportunistic pathogen Cryptococcus neoformans. In fungus, UDP-GlcA is required capsule polysaccharides, which in turn are essential virulence. The was expressed Escherichia coli and 51.3-kDa recombinant protein wild-type five mutants purified analysis. cryptococcal strongly inhibited by UDP-xylose NADH, has...
Adipocyte lipid-binding protein (ALBP) is a small (14.5 kDa) 10-stranded beta-barrel found in mammalian fat cells. The crystal structures of various holo-forms ALBP have been solved and show the fatty acid ligand bound large (approximately 400 A3) cavity isolated from bulk solvent. Examination suggests that it would be good site for creation an artificial catalyst, as numerous well defined are available past studies shown conformation to reasonably tolerant modification mutagenesis. Previous...
A number of crystallographic studies the adipocyte lipid-binding protein have established that fatty acid-binding site is within an internalized water-filled cavity. The same also suggested existence a region physically distinct from which connects cavity with external solvent, hereafter referred to as portal. In effort examine portal region, we used site-directed mutagenesis introduce mutations V32D/F57H into murine ALBP cDNA. Mutant has been isolated, crystallized, and its stability...
Currently, there is very little known about the phenotypic variability within species of Rhodotorula strains and role their capsule. Cryptococcus neoformans has been considered only encapsulated human fungal pathogen, but as more individuals come to live in states immunocompromised health, they are susceptible infections, including those by . R. mucilaginosa some most commonly associated with clinical infections. We wanted know if environmental demonstrated disparate capsule phenotypes. With...