Twenty-five years ago this month, Thomas Gold published a seminal manuscript suggesting the presence of “deep, hot biosphere” in Earth’s crust. Since publication, considerable amount attention has been given to study deep biospheres, their role geochemical cycles, and potential inform on origin life its outside Earth. Overwhelming evidence now supports biosphere ubiquitously distributed Earth both terrestrial marine settings. Furthermore, it become apparent that much is dependent...

Landfills are the final repository for most of discarded material from human society and its "built environments". Microorganisms subsequently degrade this in landfill, releasing gases (largely CH4 CO2) a complex mixture soluble chemical compounds leachate. Characterization "landfill microbiomes" their comparison across several landfills should allow identification environmental or operational properties that influence composition these microbiomes potentially biodegradation capabilities. To...

Microbial activity associated with produced water from hydraulic fracturing operations can lead to gas souring and corrosion of carbon-steel equipment. We examined the microbial ecology prospective role prevalent microorganisms in a production field Barnett Shale. The community was mainly composed halophilic, sulfidogenic bacteria within order Halanaerobiales, which reflected geochemical conditions highly saline containing sulfur species (S2O3 (2-), SO4 HS(-)). A predominant, halophilic...

Conventional water resources are not sufficient in many regions to meet the needs of growing populations. Due cyclical weather cycles, drought, and climate change, stress has increased worldwide including Southern California, which serves as a model for that integrate reuse wastewater both potable non-potable use. The Orange County Water District (OCWD) Advanced Purification Facility (AWPF) is highly engineered system designed treat produce up 100 million gallons per day (MGD) purified from...

Serpentinization can generate highly reduced fluids replete with hydrogen (H2) and methane (CH4), potent reductants capable of driving microbial methanogenesis methanotrophy, respectively. However, CH4 in serpentinized waters is thought to be primarily abiogenic, raising key questions about the relative importance methanogens methanotrophs production consumption these systems. Herein, we apply molecular approaches examine functional capability activity cycling serpentinization-impacted...

Biodiesel is a renewable substitute, or extender, for petroleum diesel that composed of mixture fatty acid methyl esters (FAME) derived from plant animal fats. Ultra-low sulfur (ULSD) blended with up to 20% FAME can be used interchangeably ULSD, compatible existing infrastructure, but also more susceptible biodegradation. Microbial proliferation and fuel degradation in biodiesel blends has been linked microbiologically influenced corrosion (MIC), this not studied previously situ. We,...

Mass-spectrometry-based metabolomics and molecular phylogeny data were used to identify a metabolically prolific strain of

Hot spring environments can create physical and chemical gradients favorable for unique microbial life. They also include authigenic mineral precipitates that may preserve signs of biological activity on Earth possibly other planets. The abiogenic or biogenic origins such be difficult to discern, therefore a better understanding formation processes is critical the accurate interpretation biosignatures from hot springs. Little Creek (LHC) complex located in Long Valley Caldera, California,...

Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom alga Picocystis sp. strain ML occurred spring 2016 at Mono Lake, hyperalkaline lake California, which was also apex multiyear-long drought. These conditions presented unique sampling opportunity investigate microbiological dynamics and potential metabolic function during an intense natural algal bloom. We conducted...

Few secondary metabolites have been reported from mammalian microbiome bacteria despite the large numbers of diverse taxa that inhabit warm-blooded higher vertebrates. As a means to investigate natural products these microorganisms, an opportunistic sampling protocol was developed, which focused on exploring isolated roadkill mammals. This initiative made possible through establishment newly created discovery pipeline, couples laser ablation electrospray ionization mass spectrometry...

Biological sulfur cycling in polar, low-temperature ecosystems is an understudied phenomenon part due to difficulty of access and the dynamic nature glacial environments. One such environment where known play important role microbial metabolisms located at Borup Fiord Pass (BFP) Canadian High Arctic. Here, transient springs emerge from ice near terminus a glacier, creating large area proglacial aufeis (spring-derived ice) that often covered bright yellow/white sulfur, sulfate, carbonate...

Bacteria associated with mammals are a rich source of microbial biodiversity; however, little is known concerning the abilities these microbes to generate secondary metabolites. This report focuses on bacterium isolated from ear feral hog southwestern Oklahoma, USA. The was identified as new strain (PE36) Brevibacillus latersporus, which shown via genomic analysis contain large number gene clusters presumably involved in metabolite biosynthesis. A scale-up culture B. latersporus PE36 yielded...

The analysis of microbial assemblages in industrial, marine, and medical systems can inform decisions regarding quality control or mitigation. Modern molecular approaches to detect, characterize, quantify microorganisms provide rapid thorough measures unbiased by the need for cultivation. requirement timely extraction high nucleic acids is faced with specific challenges when used study influence on oil production. Production facilities are often ill equipped acid techniques, making...

Abstract Ancient putative microbial structures that appear in the rock record commonly serve as evidence of early life on Earth, but details their formation remain unclear. The study modern mat can help inform properties ancient counterparts, mineralizing systems with morphological similarity to are rare. Here, we characterize partially lithified mats containing cm-scale dendrolitic coniform from a geothermal pool (“Cone Pool”) at Little Hot Creek, California, if fully lithified, would...

The genome of a member the phylum Chlorobi was assembled from shotgun metagenomic sequence hot spring in Mammoth Lakes, CA. This organism appears to be novel, aerobic, photosynthetic member, expanding knowledge this underrepresented phylum.

Microorganisms found in terrestrial subsurface environments make up a large proportion of the Earth's biomass. Biogeochemical cycles catalysed by microbes have potential to influence speciation and transport radionuclides managed geological repositories. To gain insight on factors that constrain microbial processes within formation with restricted groundwater flow we performed meta-community analysis collected from multiple discrete fractures underlying Chalk River Laboratories site (located...

The heterotrophic, epiphytic, symbiotic bacterial strain WH2K(T) was previously isolated from a two-member culture in which it attached to the heterocysts of Anabaena (SSM-00). Analysis its 16S rRNA gene sequence demonstrated that symbiont most closely related type Hoeflea marina (96.9 % similarity), belongs family Phyllobacteriaceae within order Rhizobiales class Alphaproteobacteria. A polyphasic taxonomic study performed on WH2K(T), consisted irregular rods (2-5 µm long, 0.2 wide) appeared...

Microbial mats are found in a variety of modern environments, with evidence for their presence as old the Archean. There is much debate about rates and conditions processes that eventually lithify preserve microbialites. Here we apply novel tracer experiments to quantify both mat biomass addition formation CaCO3. from Little Hot Creek (LHC), California, contain calcium carbonate formed within multiple layers, thus constitute good test case investigate relationship between rate microbial...

Abstract Background The COVID-19 pandemic highlighted the need for pathogen surveillance systems to augment both early warning and outbreak monitoring/control efforts. Community wastewater samples provide a rapid accurate source of environmental data complement direct patient sampling. Due its global presence critical missions, US military is leader in preparedness Clinical testing on Air Force (USAF) bases (AFBs) was effective but costly with respect monetary costs indirect due lost time....

Thermoanaerobaculum aquaticum MP-01(T) is currently the only cultivated and described member of Acidobacteria subdivision 23. Here, we report genome sequence for this novel microorganism that was isolated from a hot spring.

Traveler's diarrhea (TD) is a recurrent and significant issue for many travelers including the military. While known enteric pathogens exist that are causative agents of diarrhea, our gut microbiome may also play role in TD susceptibility. To this end, we conducted pilot study warfighters prior to- after deployment overseas to identify marker taxa relevant TD. This initial utilized full-length 16S rRNA gene sequencing provide additional taxonomic resolution toward identifying predictive...

Abstract Micro‐organisms have long been implicated in the construction of stromatolites. Yet, establishing a microbial role modern stromatolite growth via molecular analysis is not always straightforward because DNA stromatolites can multiple origins. For example, genomic material could represent microbes responsible for (i.e., “builders”), that inhabited structure after it was built “tenants”), or microbes/organic matter were passively incorporated from water column later diagenetic fluids...

Steep Cone Geyser is a unique geothermal feature in Yellowstone National Park (YNP), Wyoming, actively gushing silicon-rich fluids along outflow channels possessing living and silicifying microbial biomats. To assess the geomicrobial dynamics occurring temporally spatially at Cone, samples were collected discrete locations one of Cone’s for both community composition aqueous geochemistry analysis during field campaigns 2010, 2018, 2019, 2020. Geochemical characterized as an oligotrophic,...