A5005101051- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Prostate Cancer Treatment and Research
- SARS-CoV-2 detection and testing
- Acute Lymphoblastic Leukemia research
- Immunodeficiency and Autoimmune Disorders
- Chronic Myeloid Leukemia Treatments
- Sexual Differentiation and Disorders
- Galectins and Cancer Biology
- Cancer Genomics and Diagnostics
- Viral gastroenteritis research and epidemiology
- Hormonal and reproductive studies
- Urinary Bladder and Prostate Research
- Urological Disorders and Treatments
- COVID-19 Clinical Research Studies
- Prostate Cancer Diagnosis and Treatment
- Biosensors and Analytical Detection
- Tissue Engineering and Regenerative Medicine
- Advanced Breast Cancer Therapies
- PARP inhibition in cancer therapy
- Calpain Protease Function and Regulation
- Protease and Inhibitor Mechanisms
- Gut microbiota and health
- Genetic Associations and Epidemiology
Booz Allen Hamilton (United States)
2020-2024
VA Boston Healthcare System
2024
University of Chicago
2013-2019
National Heart Lung and Blood Institute
2012-2018
National Institutes of Health
2012-2018
University of Wisconsin–Madison
2017
Rensselaer Polytechnic Institute
2011
Center for Interdisciplinary Studies
2011
In vitro and in vivo studies implicate occludin the regulation of paracellular macromolecular flux at steady state response to tumor necrosis factor (TNF). To define roles these processes, we established intestinal epithelia with stable knockdown. Knockdown monolayers had markedly enhanced tight junction permeability large molecules that could be modeled by size-selective channels radii ~62.5 Å. TNF increased occludin-sufficient, but not occludin-deficient, monolayers. Complementation using...
Abstract Purpose: Germline mutations within the MEIS-interaction domain of HOXB13 have implicated a critical function for MEIS–HOX interactions in prostate cancer etiology and progression. The functional predictive role changes MEIS expression tumor progression, however, remain largely unexplored. Experimental Design: Here we utilize RNA datasets, annotated tissue microarrays, cell-based assays to investigate MEIS1 MEIS2 metastatic Results: These analyses demonstrate stepwise decrease both...
Abstract Chronic lymphocytic leukemia (CLL) is a malignancy of mature B cells driven by B-cell receptor (BCR) signaling and activated primarily in the lymph node. The Bruton's tyrosine kinase (BTK) inhibitor ibrutinib effectively inhibits BCR-dependent proliferation survival signals has emerged as breakthrough therapy for CLL. However, complete remissions are uncommon achieved only after years continuous therapy. We hypothesized that other pathways sustain CLL cell partially inhibited...
Abstract Introduction The coronavirus disease 2019 (COVID-19) pandemic is a global public health emergency causing disparate burden of death and disability around the world. viral genetic variants associated with outcome severity are still being discovered. Methods We downloaded 155 958 severe acute respiratory syndrome 2 (SARS-CoV-2) genomes from GISAID. Of these genomes, 3637 samples included useable metadata on patient outcomes. Using this subset, we evaluated whether SARS-CoV-2 genomic...
Previous studies from our lab have shown that both boric (BA) and phenylboronic- acid (PBA) inhibit the migration of prostate cancer cell lines, as well non-tumorigenic cells. Our results indicate PBA is more potent than BA in targeting metastatic proliferative properties Here we focus on impact Rho family GTP-binding proteins their downstream targets. Treatment with 1mM decreases activities RhoA, Rac1, Cdc42 DU-145 cells, but not normal RWPE-1 Furthermore, ROCKII activity phosphorylation...
Abstract Background The COVID-19 pandemic highlighted the need for pathogen surveillance systems to augment both early warning and outbreak monitoring/control efforts. Community wastewater samples provide a rapid accurate source of environmental data complement direct patient sampling. Due its global presence critical missions, US military is leader in preparedness Clinical testing on Air Force (USAF) bases (AFBs) was effective but costly with respect monetary costs indirect due lost time....
Abstract Identification of defined epithelial cell populations with progenitor properties is critical for understanding prostatic development and disease. Here, we demonstrate that Sox2 expression enriched in the cells proximal prostate adjacent to urethra. We use lineage tracing Sox2-positive during development, homeostasis, regeneration show capable self-renewal contributes regeneration. Persisting luminal express after castration, highlighting a potential role survival...
Ribavirin and lamivudine are representatives of antiviral drugs that widely used to treat viral infections, especially chronic liver disease. To compare binding mechanism behavior with human serum albumin ( HSA ), we performed fluorescence spectroscopy X‐ray crystallography investigate the interactions ribavirin . Fluorescence showed inhibit affinity each other. Our results further demonstrated lamivdudine interaction could be affected by presence other compounds, including non‐steroidal...
Abstract Mosaic loss of chromosome Y (mLOY) is a common somatic mutation in leukocytes older males. mLOY was detected 126,108 participants the Million Veteran Program: 106,054 European (EUR), 13,927 admixed African (AFR), and 6,127 Hispanic. In multi-ancestry genome-wide association analysis, we identified 323 significant loci, 167 which were novel–more than doubling number known loci. Tract-based ancestry deconvolution resolved local inflation at AFR lead SNPs. Transcriptome-wide...
Abstract Introduction The coronavirus disease 2019 (COVID-19) pandemic is a global public health emergency causing disparate burden of death and disability around the world. molecular characteristics virus that predict better or worse outcome are largely still being discovered. Methods We downloaded 155,958 severe acute respiratory syndrome 2 (SARS-CoV-2) genomes from GISAID evaluated whether variants improved prediction reported severity beyond age region. also specific to determine...
A fundamental understanding of prostate development and tissue homeostasis has the high potential to reveal mechanisms for disease initiation identify novel therapeutic approaches prevention treatment. Our current lineage specification stems from use developmental model systems that rely upon embryonic preprostatic urogenital sinus mesenchyme induce formation mature epithelial cells. It is unclear, however, how epithelium can derive both adult urethral glands epithelia. Furthermore, vast...
Electric cell-substrate impedance sensing (ECIS) is an in situ and real-time monitoring system used to detect toxic agents by changes of a confluent cell monolayer. When are introduced cells, they can cause change the barrier function, direct measure resistance current flow caused tight junction formation between cells. This exposure results immediate, quantitative measured electrodes, thus, continuously behaviour extension, exposure. We have developed ECIS-based protocol functionally...
The 2019 coronavirus disease (COVID-19) pandemic has demonstrated the importance of predicting, identifying, and tracking mutations throughout a event. As COVID-19 global surpassed one year, several variants had emerged resulting in increased severity transmissibility. Here, we used PCR as surrogate for viral load consequent to evaluate real-world capabilities genome-based clinical predictive algorithm. Using previously published algorithm, compared predictions clinically derived PCR-based...
Summary We previously interrogated the relationship between SARS-CoV-2 genetic mutations and associated patient outcomes using publicly available data downloaded from GISAID in October 2020 [1]. Using high-level included some submissions, we were able to aggregate status values differentiate severe mild COVID-19 outcomes. In our previous publication, utilized a logistic regression model with an L1 penalty (Lasso regularization) found several statistically significant associations severity....
Abstract The 2019 coronavirus disease (COVID-19) pandemic has demonstrated the importance of predicting, identifying, and tracking mutations throughout a event. As COVID-19 global surpassed one year, several variants had emerged resulting in increased severity transmissibility. In order to reduce impact on human life, it is critical rapidly identify which genetic result virulence or transmission. To address former, we evaluated if genome-based predictive algorithm designed predict clinical...
Abstract Prostate cancer is the most common type of in men, with approximately 181,000 new cases diagnosed 2016. Due to central role androgen receptor (AR) prostate development and more importantly cell survival proliferation, strategies targeting AR have been mainstay therapy for over 70 years. However, despite potent inhibition pathway activation, many patients develop castration-resistant (CRPC). Second-line therapies, such as enzalutamide, has increased overall CRPC, but resistance these...
Abstract Expression of the constitutively active androgen receptor splice variant AR-V7 is associated with resistance to anti-androgen therapies and worse clinical outcomes in prostate cancer. Dimerization a requirement for transcriptional activity, but whether that occurs through homodimerization or heterodimerization remains poorly understood. Because NR3C-family nuclear hormone receptors rely on highly conserved D-box motif within DNA-binding domain mediate dimerization, we hypothesized...
<p>Supplementary Figure Legends S1-S10</p>