Hydrogel systems based on cross-linked polymeric materials which could provide both adhesion and cohesion in wet environment have been considered as a promising formulation of tissue adhesives. Inspired by marine mussel adhesion, many researchers tried to exploit the 3,4-dihydroxyphenylalanine (DOPA) molecule cross-linking mediator synthetic polymer-based hydrogels is known be able achieve cohesive hardening well adhesive bonding with diverse surfaces. Beside DOPA residue, composition other...

During implant surgeries, antibacterial agents are needed to prevent bacterial infections, which can cause the formation of biofilms between implanted materials and tissue. Mussel adhesive proteins (MAPs) derived from marine mussels bioadhesives that show strong adhesion coating ability on various surfaces even in wet environment. Here, we proposed a novel surface-independent strategy based fusion MAP silver-binding peptide, synthesize silver nanoparticles having broad activity. This sticky...

Abstract A novel bioinspired strategy for protein nanoparticle (NP) synthesis to achieve pH‐responsive drug release exploits the pH‐dependent changes in coordination stoichiometry of iron(III)–3,4‐dihydroxyphenylalanine (DOPA) complexes, which play a major cross‐linking role mussel byssal threads. Doxorubicin‐loaded polymeric NPs that are based on Fe III –DOPA complexation were thus synthesized with DOPA‐modified recombinant adhesive through co‐electrospraying process. The doxorubicin was...

Abstract Nanofibrous scaffolds have been assessed as one of many promising tissue engineering to be utilized for wound‐healing applications. Previously, we reported multi‐functionalized electrospun nanofibrous blended with mussel adhesive protein (MAP) and polycaprolactone (PCL), which provide durable mechanical strength, cell‐friendly environments, a substantial ability capture diverse bioactive molecules without any surface modifications. In the present work, applied mats MAP PCL in vivo...

Marine mussels utilize multiple bidentate complexes formed by Fe(iii) and DOPA in a mussel adhesive protein (fp-1) to reinforce tough elastic byssal fibers as specialized underwater aid. In this study, mussel-inspired electrospun nanofibers were fabricated using recombinant (rfp-1), Fe(iii)-DOPA complexes, polycaprolactone. The mechanical properties of the reinforced complex found fp-1, which is key component naturally occurring high-performance fiber coating. Experimental results show that...

Following surgical resection for primary treatment of solid tumors, systemic chemotherapy is commonly used to eliminate residual cancer cells prevent tumor recurrence. However, its clinical outcome often limited due insufficient local accumulation and the toxicity anticancer drugs. Here, we propose a sprayable adhesive nanoparticle (NP)-based drug delivery system using bioengineered mussel protein (MAP) effective locoregional therapy. The MAP NPs could be administered target surfaces in...

Background Donor human milk should be processed to guarantee microbiological safety prior infant feeding, but this process can influence the structure and quantity of functional proteins. Objective The aim study was determine effect thawing, homogenization, vat-pasteurization (Vat-PT), retort sterilization (RTR) ultra-high-temperature (UHT) processing on bioactive proteins in donor milk. Methods Pooled either not treated (Raw) or with an additional freeze-thaw cycle without Vat-PT, RTR UHT...

Lactoferrin (LF) is a major component of human milk. LF supplementation (currently bovine) supports the immune system and helps maintain iron homeostasis in adults. No recombinant lactoferrin (rhLF) available for commercial food use. To determine extent to which rhLF (Effera™) produced by Komagataella phaffii digests similarly hmLF, validated vitro digestion protocol was carried out. Bovine (bLF) used as an additional control, it approved use various categories. This study compared intact...

The use of tissue mimics in vivo, including patterned vascular networks, is expected to facilitate the regeneration functional tissues and organs with large volumes. Maintaining patency channels contact blood an important issue development a network. Endothelium only known completely non-thrombogenic material; however, results from treatments induce endothelialization are inconclusive. present study was designed evaluate clinical applicability situ recruitment endothelial cells/endothelial...

Donor human milk (DHM) is recommended for preterm infants when a parent's own unavailable or insufficient. To ensure microbiological safety, DHM processed by donor processing companies using vat pasteurization (Vat-PT), ultra-high temperature (UHT) sterilization retort (RTR) with and without homogenization. These processes may differentially affect the bioactive proteins in DHM. LC-MS/MS-based proteomics was applied to compare detection of soluble fraction after Vat-PT, UHT sterilization,...

Human milk-protein-derived peptides exhibit an array of bioactivities. Certain bioactivities cannot be exerted unless the are absorbed across gastrointestinal lumen into bloodstream. The purpose study was to determine which derived from in vitro digestion human milk could cross intestinal Caco-2 cell monolayers. Our results showed that numbers by monolayer were different at concentrations (44 out 169 detected 10 μg/mL, 124 204 100 and 175 236 1000 μg/mL). Four (NLHLPLP (β-casein [138-144]),...

Human milk reduces risk for necrotizing enterocolitis in preterm infants. Necrotizing occurs the ileocecal region where thousands of protein-derived peptides have been released from digestion. Digestion-released may exert bioactivity, such as antimicrobial and immunomodulatory activities, gut. In this study, we applied mass spectrometry-based peptidomics to characterize present colostrum before after vitro Sequence-based computational modeling was predict with activity. We identified more...

Klebrige Sache: Neuartige Nanofaser-Kompositstrukturen auf der Grundlage von Muschel-Adhäsionsproteinen (MAPs) sind mechanisch beständig und üben Funktionen bioaktiver Peptide aus. Diese Gerüste, die auch mit vielfältigen Biomolekülen beschichtet werden können, vielversprechend für Anwendungen in Gewebezüchtung. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited or typeset. They made available...

Abstract A novel bioinspired strategy for protein nanoparticle (NP) synthesis to achieve pH‐responsive drug release exploits the pH‐dependent changes in coordination stoichiometry of iron(III)–3,4‐dihydroxyphenylalanine (DOPA) complexes, which play a major cross‐linking role mussel byssal threads. Doxorubicin‐loaded polymeric NPs that are based on Fe III –DOPA complexation were thus synthesized with DOPA‐modified recombinant adhesive through co‐electrospraying process. The doxorubicin was...

Abstract Whey protein concentrate (WPC) is consumed for its high content. The structure and biological functionality of whey proteins in WPC powders may be affected by the drying technique applied. However, specific impact spray freeze on overall profile derived from sweet streams at scale unknown. Herein, we examine effects commercial‐scale to determine which method better preserves bioactive proteins, with goal helping dairy industry create high‐value products that meet growing consumer...

Abstract Adhesion of cells to a surface is basic and important requirement in the fields cell culture tissue engineering. Previously, we constructed adhesive, fp‐151‐RGD, by fusion hybrid mussel adhesive protein, fp‐151, GRGDSP peptide, one major adhesion recognition motifs; fp‐151‐RGD efficiently immobilized on coated surfaces with no protein modifications, apparently enhanced adhesion, proliferation, spreading abilities. In present study, investigated potential use as biomimetic...