A5008155208- Aquaculture disease management and microbiota
- Natural product bioactivities and synthesis
- MicroRNA in disease regulation
- Aquaculture Nutrition and Growth
- Fish Ecology and Management Studies
- melanin and skin pigmentation
- Cancer-related molecular mechanisms research
- Phytochemistry and Biological Activities
- Fish Biology and Ecology Studies
- Genetic diversity and population structure
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Physiological and biochemical adaptations
- Biochemical Analysis and Sensing Techniques
- Sesquiterpenes and Asteraceae Studies
- Traditional and Medicinal Uses of Annonaceae
- Phytochemistry and Bioactive Compounds
- Identification and Quantification in Food
- Chemical synthesis and alkaloids
- Regulation of Appetite and Obesity
- Marine Sponges and Natural Products
- Invertebrate Immune Response Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
First Affiliated Hospital of Jiangxi Medical College
2024-2025
Nanchang University
2024-2025
Ministry of Agriculture and Rural Affairs
2019-2024
Chinese Academy of Fishery Sciences
2015-2024
Guangxi University
2022-2024
First Affiliated Hospital of Nanchang University
2024
Sapporo Medical University
2024
Shanghai Ocean University
2009-2023
Nanjing Agricultural University
2017-2023
Maternal and Child Health Hospital of Sichuan Province
2023
Abstract Red tilapia is becoming more popular for aquaculture production in China recent years. However, the pigmentation differentiation genetic breeding main problem limiting its development of commercial red culture and basis skin color variation still unknown. In this study, we conducted Illumina sequencing transcriptome on three variety tilapia. A total 224,895,758 reads were generated, resulting 160,762 assembled contigs that used as reference contigs. The had hits range 53.4% to 86.7%...
Abstract Background Grass carp ( Ctenopharyngodon idella ) belongs to the family Cyprinidae which includes more than 2000 fish species. It is one of most important freshwater food species in world aquaculture. A linkage map an essential framework for mapping traits interest and often first step towards understanding genome evolution. The aim this study construct a generation genetic grass using microsatellites SNPs generate new resource QTL economically conduct comparative analysis shed...
Delavatine A (1) is a structurally unusual isoquinoline alkaloid isolated from Incarvillea delavayi. The first and gram-scale total synthesis of 1 was accomplished in 13 steps (the longest linear sequence) commercially available starting materials. We exploited an construction strategy developed two reactions, namely Rh-catalyzed asymmetric hydrogenation indene-type tetrasubstituted olefins kinetic resolution β-alkyl phenylethylamine derivatives through Pd-catalyzed triflamide-directed C-H...
Genetic diversity within a species reflects population evolution, ecology, and ability to adapt. Genome-wide surveys of both natural introduced populations provide insights into genetic diversity, the evolutionary processes basis underlying local adaptation. Grass carp is most important freshwater foodfish for food water weed control. However, there as yet no overall picture on variations structure this species, which its aquaculture.We used 43,310 SNPs infer structure, evidence adaptation...
Differentiation and variation in body color has been a growing limitation to the commercial value of red tilapia. Limited microRNA (miRNA) information is available on skin differentiation fish so far. In this study, high-throughput Illumina sequencing sRNAs was conducted three varieties tilapia 81,394,491 raw reads were generated. A total 158 differentially expressed miRNAs (|log2(fold change)| ≥ 1 q-value ≤ 0.001) identified. Target prediction functional analysis color-related showed that...
MicroRNAs (miRNAs) are ∼22 nucleotide non-coding RNA molecules that act as crucial roles in plenty of biological processes. However, the molecular and cellular mechanisms miRNAs to regulate skin color differentiation pigmentation fish have not been fully understood. Herein, we revealed miR-206, a skin-enriched miRNA, regulates melanocortin 1 receptor (Mc1r, key regulator melanogenesis) expression by binding its 3'-untranslated (UTR) region through bioinformatics luciferase reporter assay koi...
Long non-coding RNAs (lncRNAs) perform crucial roles in biological process involving complex mechanisms. However, information regarding their abundance, characteristics and potential functions linked to fish skin color is limited. Herein, Illumina sequencing bioinformatics were conducted on black, white, red of Koi carp (Cyprinus carpio L.).
Gibberellin (GA) is an important endogenous hormone involved in plant responses to abiotic stresses. Experiments were conducted at the Research and Education Center of Agronomy, Shenyang Agricultural University (Shenyang, China) 2021.We used a pair near-isogenic inbred maize lines comprising, SN98A (light-sensitive line) SN98B (light-insensitive study effects exogenous gibberellin A3 (GA3) application on different light-sensitive under weak light conditions. The concentration GA3 was...
Abstract Background Fish reproduction, development and growth are directly affected by temperature, investigating the regulatory mechanisms behind high temperature stress is helpful to construct a finer molecular network. In this study, we systematically analyzed transcriptome miRNA information of American shad ( Alosa sapidissima ) liver tissues at different cultivation temperatures 24 ℃ (Low), 27 (Mid) 30 (High) based on high-throughput sequencing platform. Results The results showed that...
Ainsliatrimers A (1) and B (2), the first two guaianolide-type sesquiterpene lactone trimers, together with one new structurally related dimer ainsliadimer (3), were isolated from aerial part of Ainsliaea fulvioides. Their structures elucidated by spectroscopic techniques, including X-ray crystal diffraction. Both 1 2 showed potent cytotoxicites against LOVO CEM cell lines.
Abstract MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in numerous biological processes. However, the role of miRNAs antibacterial defence fish has not been fully determined. Here, we identified nine differentially expressed kidney between susceptible and resistant grass carp strains. Analysis spatial temporal miRNA expression patterns suggests cid-miRn-115 miR-142a-3p potential regulators anti-bacterial activity. Overexpressing results a visible change...
Abstract Developing metal‐free carbon catalysts is critical to achieve the electrosynthesis of hydrogen peroxide (H 2 O ) via two‐electron oxygen reduction reaction (2e − ORR) using seawater as electrolyte. Herein, N‐rich doped (NC) synthesized by directly pyrolyzing guanine both N and C sources, which can facilitate formation ultrahigh‐N dopant (up 33.89 at.%), their ratios. The NC obtained at 700 °C with hydrogenated pyridine‐N pyrrole‐N (3:2) dopants exhibits a superior selectivity H...
Background De novo transcriptome sequencing is a robust method of predicting miRNA target genes, especially for organisms without reference genomes. Differentially expressed miRNAs had been identified previously in kidney samples collected from susceptible and resistant grass carp (Ctenopharyngodon idella) affected by Aeromonas hydrophila. Target identification these differentially poses major challenge this non-model organism. Results Two cDNA libraries constructed mRNAs C. idella were...
MicroRNAs (miRNAs) are endogenous, small (21-25 nucleotide), non-coding RNAs that play important roles in numerous biological processes. Koi carp exhibit diverse color patterns, making it an ideal subject for studying the genetics of pigmentation. However, influence miRNAs on skin regulation and variation is poorly understood.Herein, we performed RNA (sRNA) analysis three main colors by Illumina sequencing. The results revealed 330, 397, 335 conserved (belonging to 81 families) 340, 353, 351...
Since the insulin-like growth factor 3 (igf3) gene was recently discovered in fish ovary, its function gonads has received much attention. In this study, we isolated two igf3 subtypes from common carp (Cyprinus carpio), which comprised full-length cDNA of 707 and 1153 nucleotides encoding 205 198 amino acids (aa), respectively. The Igf3 aa sequence had highest homology 72% with corresponding zebrafish (Danio rerio). Phylogenetic tree construction revealed that C. carpio first clustered D....