- Genomics and Phylogenetic Studies
- Fern and Epiphyte Biology
- Plant Diversity and Evolution
- Microbial Community Ecology and Physiology
- Plant and animal studies
- Chromosomal and Genetic Variations
- Algal biology and biofuel production
- Lichen and fungal ecology
- Genetic Mapping and Diversity in Plants and Animals
- Diatoms and Algae Research
- Plant and Fungal Species Descriptions
- RNA and protein synthesis mechanisms
- Ecology and Vegetation Dynamics Studies
- Lipid metabolism and biosynthesis
- Innovative Educational Techniques
- Legume Nitrogen Fixing Symbiosis
- Radiology practices and education
- Plant pathogens and resistance mechanisms
- Virus-based gene therapy research
- Cancer Immunotherapy and Biomarkers
- Medicinal Plants and Bioactive Compounds
- Higher Education and Teaching Methods
- Biocrusts and Microbial Ecology
- Genomics and Rare Diseases
- Immune cells in cancer
Gansu Agricultural University
2023
BGI Genomics
2019-2022
BGI Group (China)
2013-2022
Fujian Institute of Subtropical Botany
2018-2022
Sun Yat-sen University
2021
Institute of Botany
2012-2018
Chinese Academy of Sciences
2012-2018
Fujian Institute of Oceanography
2015-2016
Kailuan General Hospital
2016
Institute of Hydrobiology
2016
Long nanopore reads are advantageous in de novo genome assembly. However, usually have broad error distribution and high-error-rate subsequences. Existing correction tools cannot correct efficiently effectively. Most methods trim subsequences during correction, which reduces both the length of contiguity final Here, we develop an assembly tool designed to overcome complex errors reads. We propose adaptive read selection two-step progressive method quickly high accuracy. introduce a two-stage...
Here we present the genome sequence and annotation of wild olive tree (
Maize is one of the most important crops globally, and it shows remarkable genetic diversity. Knowledge this diversity could help in crop improvement; however, gold-standard genomes have been elucidated only for modern temperate varieties. Here, we present a high-quality reference genome (contig N50 15.78 megabases) maize small-kernel inbred line, which derived from tropical landrace. Using haplotype maps B73, Mo17 SK, identified 80,614 polymorphic structural variants across 521 diverse...
Abstract Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources. Here we report a reference-grade genome wild soybean accession W05, with final assembled size 1013.2 Mb and contig N50 3.3 Mb. The analytical power W05 is demonstrated by several examples. First, identify an inversion at locus determining seed coat color during domestication. Second, translocation event between chromosomes 11 13 some genotypes shown to...
Fig pollinating wasps form obligate symbioses with their fig hosts. This mutualism arose approximately 75 million years ago. Unlike many other intimate symbioses, which involve vertical transmission of symbionts to host offspring, female fly great distances transfer horizontally between In contrast, male are wingless and cannot disperse. Symbionts that keep contact hosts often show genome reduction, but it is not clear if the wide dispersal will counteract this general tendency. We sequenced...
Abstract Although long Nanopore reads are advantageous in de novo genome assembly, applying genomic studies is still hindered by their complex errors. Here, we developed NECAT, an error correction and assembly tool designed to overcome errors reads. We proposed adaptive read selection two-step progressive method quickly correct high accuracy. introduced a two-stage assembler utilize the full length of NECAT achieves superior performance both requires only 7,225 CPU hours assemble 35X...
Abstract Background Indehiscent sporangia are reported for only a few of derived leptosporangiate ferns. Their evolution has been likely caused by conditions in which promotion self-fertilization is an evolutionary advantageous strategy such as the colonization isolated regions and responds to stressful habitat conditions. The Lepisorus clathratus complex provides opportunity test this hypothesis because these ferns include specimens with regular dehiscent irregular indehiscent sporangia....
Abstract The phylogenetic relationships of the two derived fern genera Pleocnemia and Pteridrys were considered ambiguous even with molecular evidence from previous studies. In present study we determined position based on five plastid DNA regions, namely atpA , atpB rbcL rps4 + rps4–trnS intergenic spacer, trnL‐F region, an expanded taxonomic coverage including several accessions each genera. Our results showed that monophyletic genus belonged to Dryopteridaceae was not related...
Using DNA sequences of the choroplast genome, we addressed phylogenetic relationships two understudied fern species belonging to pteridoid clade Pteridaceae. The studied occur in Afro-Madagascar and Sino- Himalaya respectively. Our results recovered a sister relationship Sino-Himalayan Cerosora microphylla Afromadagascan Pityrogramma argentea. latter is not closely related predominantly Neotropical genus transferred Cerosora. also confirmed Actiniopteris Onychium. These contribute rapidly...
摘要: 石松类和蕨类植物是以孢子繁殖的维管植物, 在陆地植物演化上占据重要地位。随着分子系统学研究的开展, 各大类群间的系统发育关系得以阐明, 传统上的概念得以修正, 新的现代石松类和蕨类植物的分类系统也被提出, 并不断得到完善。该文介绍国内外在蕨类植物系统发育方面的研究成果, 重点讨论中国分布的类群的分类处理。文中提出了一个完整的中国现代石松类和蕨类植物的分类系统, 包括5亚纲、14目、39科及12亚科、约140余属。
Abstract Homoplasy, autapomorphy and rapid radiation have frequently been discussed as the main causes of instable generic classifications plants including ferns. In this study, we explore natural classification for selligueoid ferns by investigating phylogenetic relationships among almost all its previously recognized segregates. We compare phylogenies that resulted from two datasets. Dataset 1 comprises a broad taxonomic sampling fifty–one species Phylogenetic analysis dataset revealed...
Abstract The origin of the latitudinal biodiversity gradient has been studied using various approaches. Here, we employ a comparative phylogenetic approach to infer evidence for hypothesis that differences in diversification rates are one main factors contributing assembly this gradient. We phylogeny two sister genera Phegopteris and Pseudophegopteris . distinct their species richness (4 vs. 20 spp.) preferences temperate subtropical ( ) or tropical climates ). Using sequences three plastid...
Schizochytrium sp. TIO1101 is a crucial commercial alga used to produce docosahexaenoic acid (DHA), long-chain polyunsaturated fatty that beneficial for human health. In this study, we sequenced the mitochondrial genome (mitogenome) of first time using an Illumina HiSeq 2500 system (Illumina Inc., San Deigo, CA). The assembled mitogenome was 31 494 bp long with 33.92% GC content. contains 56 genes, including 33 protein-coding 21 transfer RNA genes and two ribosomal genes. Maximum-likelihood...
Breast cancer is the most prevalent malignancy globally in women, and tumor metastasis a major cause of high mortality advanced breast cancer. Immune components microenvironment can modulate progression, which could be attractive therapeutic targets. However, composition characterization ovarian was unknown. Here, single-cell RNA sequencing used to detect cancerous paracancerous tissue samples from 10 cell types 17 clusters were identified, including T cell, B & Plasma, Macrophage,...
Generating chromosome-scale haplotype resolved assembly is important for functional studies. However, current de novo assemblers are either haploid that discard allelic information, or diploid can only tackle genomes of low complexity.Here, Using robust programs, we build a genome pipeline called gcaPDA (gamete cells assisted Phased Diploid Assembler), which exploits gamete to assist in resolving haplotypes. We demonstrate the effectiveness based on simulated HiFi reads maize highly...
Pectinodesmus pectinatus is a green alga of commercial interest in sewage purification. Clarification its organelle genomes helpful for genetic manipulation, taxonomic revisions and evolutionary research. Here, de novo sequencing was used to determine chloroplast genome mitochondrial P. strain F34. The composed large single-copy (LSC) region 99,156 bp, small (SSC) 70,665 pair inverted repeats (IRs) with length 13,494 bp each separated by LSC SSC. contained 69 protein-coding genes, 25...
The complete mitochondrial genome of the green algae Hariotina sp. F30 was obtained in this study using Illumina sequencing data. It is 51 915 bp length with 36.23% GC content. contains 13 protein-coding genes, 23 tRNA genes and six rRNA all which are encoded on heavy strand. AUG a universal initiation codon among genes. UCA termination for most gens except UAA cox1 cob UGA nad6 gene. CUU anticodon tRNA-Lys detected first time Sphaeropleales.
Abstract A unicellular marine diatom,
With the improvement of China's comprehensive national strength, process internationalization higher education has been accelerating and scale expanding.The key to success or failure clinical medical students lies in quality education, so it is very important establish improve teaching assurance monitoring system.Based on author's practice, this paper puts forward system undergraduate medicine, namely: Improving management system, mechanism rules regulations foreign students; Strengthening...