A5042641990- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Diabetes Treatment and Management
- Animal Nutrition and Physiology
- Diet and metabolism studies
- Ion channel regulation and function
- Drug Transport and Resistance Mechanisms
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Diabetes and associated disorders
- Diabetes Management and Research
- Cardiac electrophysiology and arrhythmias
- Diet, Metabolism, and Disease
- Metabolism and Genetic Disorders
- Growth Hormone and Insulin-like Growth Factors
- Cardiovascular Function and Risk Factors
- Mitochondrial Function and Pathology
- Salivary Gland Disorders and Functions
- Bone health and osteoporosis research
- Historical Medical Research and Treatments
- Autophagy in Disease and Therapy
- Agriculture Sustainability and Environmental Impact
- Chronic Kidney Disease and Diabetes
- Genetics, Aging, and Longevity in Model Organisms
- Adrenal and Paraganglionic Tumors
- Ethics in Clinical Research
University of Kentucky
2016-2025
National University of Life and Environmental Sciences of Ukraine
2023
Université Libre de Bruxelles
2013-2015
Institutul Național de Endocrinologie C.I. Parhon
2015
Carol Davila University of Medicine and Pharmacy
2014
Zero to Three
2014
Leefmilieu Brussel
2013
Inserm
2010-2011
Université Lille Nord de France
2010-2011
Institut Pasteur de Lille
2010-2011
Bile acids (BA) participate in the maintenance of metabolic homeostasis acting through different signaling pathways. The nuclear BA receptor farnesoid X (FXR) regulates pathways BA, lipid, glucose, and energy metabolism, which become dysregulated obesity. However, role FXR obesity associated complications, such as dyslipidemia insulin resistance, has not been directly assessed.Here, we evaluate consequences deficiency on body weight development, lipid resistance murine models genetic...
Farnesoid X receptor (FXR) is highly expressed in liver and intestine where it controls bile acid (BA), lipid glucose homeostasis. Here we show that FXR functional, as assessed by target gene expression analysis, human islets β‐cell lines. predominantly cytosolic‐localized the of lean mice, but nuclear obese mice. Compared to FXR+/+ FXR−/− mice display a normal architecture mass certain islet‐specific genes altered. Moreover, glucose‐stimulated insulin secretion (GSIS) impaired Finally,...
Human induced pluripotent stem cells (iPSCs) offer hope for personalized regenerative cell therapy in amyotrophic lateral sclerosis (ALS). We analyzed the fate of human iPSC-derived neural progenitors transplanted into spinal cord wild-type and transgenic rats carrying a mutated SOD1(G93A) gene. The aim was to follow survival differentiation until day 60 post-transplantation two different vivo environments, one being ALS-like. efficiently engrafted adult survived at high numbers. Different...
To investigate the role of interleukin (IL)-33-ST2 axis in pathophysiology primary Sjögren's syndrome (pSS).Serum levels IL-33 and sST2 were determined by ELISA. The expression ST2 was investigated salivary glands (SG) immunohistochemistry. PBMC isolated stimulated with IL-33, IL-12 IL-23 cytokine profile response examined flow cytometry. Intracellular detection IFNγ IL-17 performed cytometry.Serum increased pSS patients compared controls systemic lupus erythematosus. Expression upregulated...
Loss-of-function mutations in the cytoskeletal protein ankyrin-B (AnkB) cause ventricular tachyarrhythmias humans. Previously, we found that a larger fraction of sarcoplasmic reticulum (SR) Ca 2+ leak occurs through sparks AnkB-deficient (AnkB +/− ) mice, which may contribute to arrhythmogenicity via waves. Here, investigated mechanisms responsible for increased spark frequency AnkB hearts. Using immunoblots and phospho-specific antibodies, phosphorylation ryanodine receptors (RyRs) by...
Abstract The relationship between osteoblast-specific insulin signaling, osteocalcin activation and gluco-metabolic homeostasis has proven to be complex potentially inconsistent across animal-model systems in humans. Moreover, the impact of postnatally acquired, deficiency on pancreas-to-skeleton-to-pancreas circuit not been studied. To explore this relationship, we created a model postnatal elimination signaling osteoprogenitors. Osteoprogenitor-selective ablation receptor was induced after...
Background/Aim: The submandibular gland is one of the three major salivary glands, producing a mixed secretion; this saliva hypotonic compared to plasma. It also secretes glucose, but mechanisms responsible for process are poorly understood. Our study addressed question whether glucose transporters expressed and how they localized within specific rodent cells, in order estimate possible implication disposal. Methods: Immunohistochemistry, RT-qPCR Western blotting were performed determine...
SGLT2 inhibitors reduce insulin resistance and may improve beta-cell function in humans with T2D. We studied the effects of Canagliflozin (Cana) on glucose homeostasis, islet architecture endocrine cell fate male TallyHO/JngJ (TH) mice, a new model T2D, which mimics many aspects polygenic T2D humans. By 8 weeks age, all TH mice developed moderate obesity hyperglycemia compared to control SWR/J mice; ∼60% converted quickly overt diabetes, characterized by elevated BG values (>400mg/dL)...
The non-invasive imaging of GLUT2-expressing cells remains a challenge. As streptozotocin, and similarly alloxan, may be transported into by GLUT2, the major aim present study was to assess possible use fluorescent desnitroso-streptozotocin analogs for in vitro labeling cells. INS-1E cells, human embryonic kidney (HEK) rat isolated pancreatic islets, hepatic exocrine tumoral insulin-producing BRIN-BD11 were incubated presence two distinct analogs, probes A B. immunocytochemistry GLUT2...