A5089352302- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- Pancreatic function and diabetes
- Biosensors and Analytical Detection
- RNA modifications and cancer
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Organ Transplantation Techniques and Outcomes
- Liver physiology and pathology
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Advanced Nanomaterials in Catalysis
- Gastric Cancer Management and Outcomes
- Advanced Photocatalysis Techniques
- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Mass Spectrometry Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Mesenchymal stem cell research
- Advanced Chemical Sensor Technologies
Peking University Shenzhen Hospital
2006-2024
University of Hong Kong - Shenzhen Hospital
2018-2024
Nanjing Sport Institute
2024
Nanjing University of Posts and Telecommunications
2023-2024
Nanjing Foreign Language School
2022
Fuling Center Hospital of Chongqing
2022
Huazhong University of Science and Technology
2021
Anhui Medical University
2018
South China Agricultural University
2012-2018
Peking University
2006-2017
The abundant and reversible N6-methyladenosine (m6A) RNA modification its modulators have important roles in regulating various gene expression biological processes. Here, we demonstrate that fat mass obesity associated (FTO), as an m6A demethylase, plays a critical anti-tumorigenic role clear cell renal carcinoma (ccRCC). FTO is suppressed ccRCC tissue. low of human correlates with increased tumour severity poor patient survival. Von Hippel-Lindau-deficient cells expressing restores...
Herein, G-quadruplex sequence was found to significantly decrease the diffusion current of methylene blue (MB) in homogeneous solution for first time. Electrochemical methods combined with circular dichroism spectroscopy and UV–vis were utilized systematically explore interaction between MB an artificial sequence, EAD2. The EAD2 (the binding constant, K ≈ 1.3 × 106 M–1) stronger than that double-stranded DNA (dsDNA) (K 2.2 105 M–1), stoichiometry (n) EAD2/MB complex calculated be 1.0...
Various studies demonstrate that long noncoding RNAs (IncRNAs) act as oncogenes or tumor suppressors in cancer. However, the function of IncRNAs bladder cancer still remains largely unknown. In this study, we identified an IncRNA, gastric cancer–associated IncRNA1 (GClnc1), which was high abundance tissues and its expression related to poor survival rates patients with vitro vivo assays showed GClnc1 significantly promoted cell proliferation, metastasis, invasiveness Mechanistically, first...
Abstract Cullin-RING E3 ubiquitin ligase (CRL) family members play critical roles in numerous biological processes and diseases including cancer Alzheimer’s disease. Oligomerization of CRLs has been reported to be crucial for the regulation their activities. However, structural basis its mechanism oligomerization are not fully known. Here, we present cryo-EM structures oligomeric CRL2 FEM1B unneddylated state, neddylated state complex with BEX2 as well FNIP1/FLCN. These reveal that...
We first developed a label-free and immobilization-free homogeneous electrochemical aptasensor, which combined smart functional DNA hairpin designed miniaturized device. Cocaine was chosen as model target. The anticocaine aptamer peroxidase-mimicking DNAzyme were integrated into one single-stranded hairpin. Both G-quadruplex elaborately blocked by the stem region. conformation switching induced affinity interaction between cocaine released part turned on activity. device, constructed...
Increasing evidence indicated that metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) acted as a key regulator in the proliferation and invasion of several cancers. However, function MALAT1 development cholangiocarcinoma has not been experimentally established. In present study, expression levels cell lines were detected by quantitative real-time PCR. The effects knockdown on cells with Cell Counting Kit-8 (CCK-8), colony formation assay Trans-well assay, respectively....
Renal cell carcinoma (RCC) is a most common kidney malignancy, with atypical symptoms in the early stage and poor outcome late stage. Recently, emerging evidence revealed that some miRNAs play an essential role tumorigenesis progression of RCC. Therefore, aim this study understand detailed molecular mechanism miR-23a-3p RCC identify its potential clinical value.
Renal cell carcinoma (RCC) accounts for more than 90% of cancers in the kidney. RCC is often asymptomatic, as a result people with generally have advanced disease by time it discovered and has poor prognosis compared to other cancers. Therefore, necessary explore its pathogenesis identify some reliable prognostic biomarker RCC. miRNAs are emerging important players development progression miR-31-5p been reported act tumor suppressor hepatocellular (HCC). The aim this study determine detailed...
To investigate the stepwise development and germ cell gene expression in allografted neonatal mouse testes differentiation of immature human testicular cells xenografted testes.Immunodeficient nude mice were used as hosts for allografting xenografting fetal tissues. Stepwise stage-specific allografts systematically evaluated parallel compared with those intact by periodically monitoring graft status measurement weight, histological analysis determination five genes. Human tissues from 20 26...
Background: Bladder cancer (BC) is one of the most common malignancies world-wide with high morbidity and mortality.Long noncoding RNAs (lncRNAs) are thought to play a critical role in development.LncRNA NRON, repressor activated T-cell nuclear factor (NFAT), has been shown be dysregulated many types.However, clinical significance molecular mechanism NRON bladder still unknown.Methods: The expression levels BC tissues cell lines were tested by RT-qPCR.Survival analysis was performed detect...
A simple and novel reporter-triggered isothermal exponential amplification reaction (R-EXPAR) integrated with a miniaturized electrochemical device was developed, which achieved excellent improvement (five orders of magnitude) sensitivity toward reporter, G-quadruplex. This R-EXPAR strategy has been successfully implemented to construct homogeneous label-free sensor for ultrasensitive DNA detection.
Abstract Metastasis is the main cause of cancer-associated deaths, yet this complex process still not well understood. Many studies have shown that acetate involved in cancer metastasis, but molecular mechanisms remain to be elucidated. In present study, we first measured effect on zinc finger transcriptional repressor SNAI1 and acetyl-CoA synthetase 2 (ACSS2) under glucose limitation renal cell carcinoma lines, 786-O ACHN. Then, RNA interference overexpression ACSS2 were used detect role...
An increasing number of studies have demonstrated the function microRNAs (miRNAs) in initiation and development various types cancer. Among them, miR-425-5p is proven to serve an important several cancer, including gastric, cervical hepatocellular carcinoma. However, renal cell carcinoma (RCC) remains unclear. In present study, it was that expression level upregulated RCC tissues lines compared with normal (P<0.05). Additionally, Cell Counting kit-8 MTT assays were employed assess viability...
Herein, we proposed a portable, easy-to-operate, and antifouling microcapsule array chip for target detection. This prepackaged was fabricated by innovative cost-effective 3D ice printing integrating with photopolymerization sealing which could eliminate complicated preparation of wet chemistry effectively resist outside contaminants. Only small volume sample (2 μL each microcapsule) consumed to fulfill the assay. All reagents required analysis were stored in form within before use,...
Sow milk yield and quality is crucial for the survival growth of piglets. To understand molecular mechanisms lactogenesis lactation, mammary tissue samples were taken from six sows at −17(±2), 1 17(±2) days relative to parturition. Mammary tissues two in same stage used extract RNA, which subsequently pooled equal amounts. Nine hybridized porcine Affymetrix GeneChips. Totally 1,524 genes detected as significantly differentially expressed over time course tested (p<0.01, q<0.01, fold change...
AIM:To investigate intraperitoneal transplantation of microencapsulated hepatic-like cells from human umbilical cord blood for treatment hepatic failure in rats. METHODS: CD34+ were isolated by magnetic cell sorting.In the vitro
Previous studies have indicated that the deregulation of microRNAs contributes to tumorigenesis. Misregulation microRNA-520e (miR-520e) has been observed in various types cancer. However, expression profile and biological function miR-520e breast cancer remains largely unknown. The present study demonstrated was significantly increased tissues compared with adjacent non-cancerous 21 patients, as revealed by reverse transcription-quantitative polymerase chain reaction. Furthermore,...