A5066892312- Sleep and Wakefulness Research
- Genetics and Neurodevelopmental Disorders
- Circadian rhythm and melatonin
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- Sleep and related disorders
- TGF-β signaling in diseases
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Regulation of Appetite and Obesity
- Biochemical and Molecular Research
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Cancer-related gene regulation
- Amino Acid Enzymes and Metabolism
- Galectins and Cancer Biology
- DNA Repair Mechanisms
- Biochemical Analysis and Sensing Techniques
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
- Neurobiology and Insect Physiology Research
- Adipose Tissue and Metabolism
- ATP Synthase and ATPases Research
- Kruppel-like factors research
- Neuroinflammation and Neurodegeneration Mechanisms
Tsukuba International University
2016-2025
University of Tsukuba
2015-2025
Korea Advanced Institute of Science and Technology
2025
CHA University
2012-2015
Recent advances in genome and transcriptome analysis have contributed to the identification of many potential cancer-related genes. Furthermore, biological clinical investigations candidate genes provide us with a better understanding carcinogenesis development cancer treatment. Here, we report novel role KIAA1324 as tumor suppressor gastric cancer. We observed that was downregulated most cancers from sequencing data found histone deacetylase involved suppression KIAA1324. Low levels were...
Orexins are hypothalamic neuropeptides that regulate feeding, energy expenditure, and sleep. Although orexin-deficient mice susceptible to obesity, little is known about the roles of orexin receptors in long-term metabolism. Here, we performed metabolic characterization receptor-deficient mice. Ox1r-deficient were resistant diet-induced their food intake was similar between chow high-fat food. Ox2r-deficient exhibited less expenditure than wild-type when fed a diet. Neither nor showed body...
Mammals exhibit circadian cycles of sleep and wakefulness under the control suprachiasmatic nucleus (SCN), such as strong arousal phase-locked to beginning dark phase in laboratory mice. Here, we demonstrate that salt-inducible kinase 3 (SIK3) deficiency gamma-aminobutyric acid (GABA)-ergic neurons or neuromedin S (NMS)-producing delayed peak lengthened behavioral cycle both 12-h light:12-h condition (LD) constant (DD) without changing daily amounts. In contrast, induction a gain-of-function...
KIAA1324 is a transmembrane protein largely reported as tumor suppressor and favorable prognosis marker in various cancers, including gastric cancer. In this study, we report the role of N-linked glycosylation functional post-translational modification (PTM). Loss eliminated potential to suppress cancer cell proliferation migration. Furthermore, demonstrated that undergoes fucosylation, N-glycan mediated by fucosyltransferase, inhibition fucosylation also significantly suppressed...
Significance The network of genes and molecules that govern sleep wakefulness remains largely unknown. Forward genetics is a powerful approach to elucidate important biological phenomena cannot be predicted from the function known genes. We established large-scale screening system using EEG/EMG-based sleep/wake monitoring with reliable parameters. search for novel has succeeded in identifying several regulatory By furthering study, more comprehensive picture pathways sleep/wakefulness will...
There are various sex differences in sleep/wake behaviors mice. However, it is unclear whether there sleep homeostasis and arousal responses gonadal hormones involved these differences. Here, we examined under baseline condition, after deprivation by gentle handling, to repeated cage changes male female C57BL/6 mice that hormonally intact, gonadectomized, or gonadectomized with hormone supplementation. Compared males, females had longer wake time, shorter non-rapid eye movement (NREMS) rapid...
Cyclin-dependent kinase-like 5 (CDKL5) deficiency disorder (CDD) is an X-linked rare neurodevelopmental associated with severe sleep disturbances. However, little known about the mechanisms underlying disturbances in CDD patients. Here, we employed electroencephalogram (EEG) recording to characterize sleep–wake behaviors and EEG activity male CDKL5-deficient mice. We found that young adult middle-aged Cdkl5 knockout (KO) mice recapitulated phenotypes patients CDD, including difficulties...
The discovery of leptin substantiated the usefulness a forward genetic approach in elucidating molecular network regulating energy metabolism. However, no successful dominant screening for obesity has been reported, which may be due to influence quantitative trait loci between and counter strains low fertility obese mice. Here, we performed using C57BL/6 substrains, C57BL/6J C57BL/6N, with routine use vitro fertilization. more than 5000 mutagenized mice established two pedigrees single...
In most of human cancer, the c-Myc proto-oncogene is highly activated. Dysregulation oncoprotein contributes to drive tumorigenesis in numerous tissues and organs. Thus, targeting stability can be a critical step for cancer therapy. Here we report Smad7 as key molecule regulate activity by recruiting F-box protein, Skp2. Ectopic expression down-regulated protein level without affecting transcription significantly repressed its transcriptional activity, leading inhibition cell proliferation...
Abstract PANcreatic-DERived factor (PANDER, FAM3B) is a member of the FAM3 family cytokine molecules. FAM3B expression originally identified in endocrine pancreas and also expressed colon stomach. Initial characterization revealed potential role pancreatic islet apoptosis. However, biological function has still remained elusive. Here we show that level decreased 11 out 14 cancer tissues samples (78.5%) as well gastric cell lines. Ectopic leads to inhibition growth reduction single colony...
Abstract The cellular response to DNA damage is essential in maintaining genomic stability. As accumulates, the occurrence rate of cancer significantly enhanced. Smad7 known as an inhibitory Smad which negatively regulates TGF-β-induced signaling pathway. predominantly localized nucleus even without TGF-β stimulation. However, TGF-β-independent function still not clear. Here, we report double-strand break induced by radiomimetic drug, neocarzinostatin (NCS). overexpression accelerates repair...
<p>Supplementary Figures S1-S13 and Table S1. Confirmation of KIAA1324 mRNA expression in primary tissues from gastric cancer (GC) patients GC cell lines (S1). Analysis correlation between copy number variation (CNV) (S2). enhanced cisplatin- etoposide-induced apoptosis cells (S3). knockdown reduced staurosporine-induced (S4). did not have a considerable effect on cycle (S5). affect autophagy (S6). â–³C caused growth inhibition (S7). â–³N induce (S8). 1-303 a.a. was required for...
<p>Supplementary Figures S1-S13 and Table S1. Confirmation of KIAA1324 mRNA expression in primary tissues from gastric cancer (GC) patients GC cell lines (S1). Analysis correlation between copy number variation (CNV) (S2). enhanced cisplatin- etoposide-induced apoptosis cells (S3). knockdown reduced staurosporine-induced (S4). did not have a considerable effect on cycle (S5). affect autophagy (S6). â–³C caused growth inhibition (S7). â–³N induce (S8). 1-303 a.a. was required for...
<div>Abstract<p>Recent advances in genome and transcriptome analysis have contributed to the identification of many potential cancer-related genes. Furthermore, biological clinical investigations candidate genes provide us with a better understanding carcinogenesis development cancer treatment. Here, we report novel role KIAA1324 as tumor suppressor gastric cancer. We observed that <i>KIAA1324</i> was downregulated most cancers from sequencing data found histone...
<p>Supplementary Materials and Methods</p>
<div>Abstract<p>Recent advances in genome and transcriptome analysis have contributed to the identification of many potential cancer-related genes. Furthermore, biological clinical investigations candidate genes provide us with a better understanding carcinogenesis development cancer treatment. Here, we report novel role KIAA1324 as tumor suppressor gastric cancer. We observed that <i>KIAA1324</i> was downregulated most cancers from sequencing data found histone...
<p>Supplementary Materials and Methods</p>
Abstract KIAA1324 is a transmembrane protein reported largely as tumor suppressor and favorable prognosis marker in various cancers, including gastric cancer. In this study, we report the role of N-linked glycosylation functional post-translational modification (PTM). Loss eliminated potential to suppress cancer cell proliferation migration. addition, KIAA1324-mediated apoptosis regression were inhibited by loss glycosylation. The non-glycosylated mutant also showed altered localization lost...
Abstract CBL protein family functions as multifunctional adaptor proteins and E3 ubiquitin ligases that are implicated regulators of signaling in various cell types. Recent discovery revealed mutations proto-oncogenic the linker region RING finger domain human acute myeloid neoplasm these transforming induced carcinogenesis. However, function mediated pathway during tumorigenesis has not been well characterized. Here, we show highly expresses breast cancer cells significantly inhibits TGF-β...