Sex steroids are required for a normal pubertal growth spurt and fusion of the human epiphyseal plate. However, localization sex steroid receptors in plate remains controversial. We have investigated expression estrogen receptor (ER) alpha, ERbeta androgen (AR) biopsies proximal tibial plates obtained during surgery 16 boys eight girls. All stages were represented (Tanner 1-5). ERalpha, AR visualized with immunohistochemistry number receptor-positive cells was counted using an image analysis...

To identify chromosomal regions that may contain loci for tumor suppressor genes involved in adrenocortical development, a panel of 60 tumors (39 carcinomas and 21 adenomas) were screened loss heterozygosity. Although the vast majority heterozygosity (LOH) detected chromosomes 2, 4, 11, 18, only few found adenomas. Therefore, 2 harbor familial cancer syndromes Carney complex 2p16 multiple endocrine neoplasia type 1 gene 11q13 further studied 27 (13 14 tumors. Detailed analysis region mapped...

We studied the involvement of ERK cascade in human chorionic gonadotropin (hCG)-induced steroidogenesis by primary cultures immature rat Leydig cells. Our findings indicate that protein kinase A and C function as upstream kinases connection with transduction signal from receptor to cascade. These MAPKs enhance stimulatory effects hCG on de novo synthesis steroidogenic acute regulatory activity phosphatase 2A, which are associated increased androgen production cell. Specific inhibition ERK1/2...

BACKGROUND Various mediators of pruritus have been suggested that might be responsible for the mechanism in psoriasis.To study expression levels members tachykinin family, substance P and neurokinin (NK) A their receptors, NK-1 NK-2, psoriasis to correlate with intensity pruritus. possible correlation chronic stress depression was also evaluated.Biopsies were obtained from 28 patients plaque psoriasis; majority had The samples taken lesional nonlesional areas on back 10 healthy controls,...

Atopic dermatitis (AD) is a chronic inflammatory skin disorder. It often reported to be worsened by psychological stress.To explore the role of stress and related triggers in AD, its connection worsening this disease, focusing on patients' perspectives.In total, 28 patients with AD were included focus groups. Topics regarding discussed.The hypothesis that may have impact eczema pruritus was supported all patients. Distinguishing effect from effects physiological triggers, such as infection,...

Atopic dermatitis (AD) is a chronic, itchy, inflammatory skin disorder that may worsen due to stress and anxiety. Tachykinins have been suggested be involved in the inflammation AD, as well pruritus. Aprepitant NK-1 receptor antagonist. This open randomized trial evaluated effect of aprepitant added topical treatment adult patients with moderate?severe AD. The group (n?=?19) received 80 mg/day for 7 days supplement standardized moderately strong steroid moisturizer. control (n?=?20) alone....

Maria H. Lönnkvista, Elvar Theodorssonb, Mikael Holstc, Tryggve Ljungd & Per M. Hellström*ea Department of Medicine, Gastroenterology and Hepatology Unit, Karolinska University Hospital Solna, Swedenb Clinical Experimental Medicine/Clinical Chemistry, Linköping University, Linköping, Swedenc Woman Child Health, Swedend Merck, Sharp Dohme, Stockholm, Swedene Medical Sciences, Uppsala Hospital, Uppsala, Sweden

Background Atopic dermatitis (AD) is a chronic, inflammatory, often severely itching skin disorder. It may worsen due to stress, depression, or anxiety. Calcitonin gene-related peptide (CGRP) be involved in inflammation signaling. CGRP has also been suggested relation and This study aimed investigate the expression of patients with AD.Methods Twenty-seven adult AD, characterized clinical psychodemographic parameters, were investigated regarding biopsies, using an immunohistochemical...

The distribution of rapidly phosphorylated chromosomal proteins between chromosome I, II + III, IV, and nuclear sap including the matrix was investigated in salivary gland cells Chironomus tentans. Chromosome which carries most active nonribosomal genes cell, found to be enriched four nonhistone polypeptides (Mr = 25,000, 30,000, 33,000, 42,000) parallel with transcriptional activity rather than DNA content chromosome. Also histones H2A H4 are but phosphorylation is proportional each sample....

Chronic lung disease (CLD) of prematurity is a prolonged respiratory failure in very-low-birth-weight neonates. Proinflammatory cytokines have been implicated the development CLD. Steroids shown to produce some improvement neonates with this disease. The purpose study was evaluate downregulation these proinflammatory by dexamethasone, budesonide and recombinant IL-10 (rIL-10) order elucidate mechanism clinical benefit steroids babies. Our results showed that rIL-10 significantly inhibited...

Abstract Aim: Nitric oxide (NO) synthesis and inducible NO synthase (NOS) expression are increased in colon of patients with inflammatory bowel disease (IBD) associated decreased contractility. The aim was to investigate which subtype NOS that is activated experimental colitis. Methods: Experimental colitis induced Sprague–Dawley rats by Escherichia coli endotoxin. Expression different subtypes compared normal inflamed using reverse transcriptase‐polymerase chain reaction. In organ baths,...

Context: Human mastocytosis is a rare disease, in which the serotonergic system may be involved.Objective: The objective of present study was to examine possible presence serotonin (5-HT) and its 5-HT1A receptor (R) skin patients with mastocytosis. In addition, effect 5-HT1AR tested on human cells, cultured vitro.Materials methods: expression 5-HT urticaria pigmentosa mastocytoma studied using immunohistochemistry. effects 8-OH-DPAT, an agonist 5-HT1AR, proliferation (cell number),...

Context: Atopic dermatitis (AD) is a chronic, inflammatory, itching skin disorder, which may worsen due to stress, depression and anxiety. Tachykinins be involved in inflammation signaling as well they have role anxiety.Objective: This study aimed measure the expression of tachykinin markers, patients with AD, correlation these tachykinins clinical psychodemographic parameters.Materials methods: Twenty-eight adult AD were investigated regarding biopsies, using an immunohistochemical...

The symptoms of atopic dermatitis (AD) are often aggravated by anxiety, and the serotonin transporter (5-HTT) has been shown to be importance in this context. Three polymorphisms affecting transcription gene known: a repetitive element, promoter region (5HTTLPR), variable number tandem repeats (VNTR) within intron 2 referred as STin2, single-nucleotide (A/G) polymorphism (SNP) located 5-HTTLPR.To examine for possible relationships between these aggravation AD stress.Thirty-three patients...

Abstract Background Stress is known to worsen the symptoms of atopic eczema (AE). Substance P likely play an important role in development and pathogenesis AE. Objective To examine a possible connection between chronic mild stress changes expression substance its receptor (R) neurokinin (NK) 1 skin stress‐related brain regions NC/Nga atopic‐like mice. Methods The mice were divided into three groups (eight animals per group): SE (stressed eczematous), NSE (non‐stressed eczematous) SC...

Background: Fecal calprotectin (FC) and serum C-reactive protein (CRP) are biomarkers of disease activity in Crohn's (CD) ulcerative colitis (UC). We assessed FC, CRP, Harvey–Bradshaw index (HBi), partial Mayo Clinic Scoring (pMCS) a cytokine panel during infliximab induction to predict therapy outcome.Methods: CRP clinical indices were evaluated 123 (76 CD, 47 UC) patients before after 12 weeks. Responders monitored 48 weeks for an 'incident' (dosage increase, shortened dosage interval,...

Abstract The rapidly turning over phosphorylation of specific nuclear nonhistone proteins, especially 42‐, 33‐, and 30‐kDa polypeptides, its relation to the transcriptional activity hnRNA genes was investigated in isolated nuclei from salivary gland cells Chironomus tentans . Incubation conditions promoting proteins as well RNA polymerase II were established. pattern 32 P incorporation into found resembled that obtained experiments with intact cells, endogenous retained ability reinitiate...