A5028764160- Inflammasome and immune disorders
- interferon and immune responses
- Immune Response and Inflammation
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- NF-κB Signaling Pathways
- Reproductive tract infections research
- Exercise and Physiological Responses
- Immune Cell Function and Interaction
- Cell death mechanisms and regulation
- Adenosine and Purinergic Signaling
- Muscle Physiology and Disorders
- Autophagy in Disease and Therapy
- Heme Oxygenase-1 and Carbon Monoxide
- IL-33, ST2, and ILC Pathways
- Cardiovascular Disease and Adiposity
- Cytokine Signaling Pathways and Interactions
- Reproductive System and Pregnancy
- T-cell and B-cell Immunology
- Mitochondrial Function and Pathology
- RNA regulation and disease
- Chronic Lymphocytic Leukemia Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Gout, Hyperuricemia, Uric Acid
- Adolescent and Pediatric Healthcare
York University
2016-2025
Krirk University
2023
University of Toronto
2014-2017
Centre for Social Innovation
2016
American University of Beirut
2015
Columbia University
2011-2015
University of California, Merced
2007-2012
Extracellular adenosine triphosphate (ATP) can activate purinergic receptors of the plasma membrane and modulate multiple cellular functions. We report that ATP is released from HIV-1 target cells through pannexin-1 channels upon interaction between envelope protein specific cell receptors. then acts on receptors, including P2Y2, to proline-rich tyrosine kinase 2 (Pyk2) transient depolarization, which in turn stimulate fusion Env-expressing membranes containing CD4 plus appropriate chemokine...
Chlamydia trachomatis infections cause severe and irreversible damage that can lead to infertility blindness in both males females. Following infection of epithelial cells, induces production reactive oxygen species (ROS). Unconventionally, Chlamydiae use ROS their advantage by activating caspase-1, which contributes chlamydial growth. NLRX1, a member the Nod-like receptor family translocates mitochondria, augment from mitochondria following Shigella flexneri infections. However, general,...
Production of IL-1beta typically requires two-separate signals. The first signal, from a pathogen-associated molecular pattern, promotes intracellular production immature cytokine. second derived danger signal such as extracellular ATP, results in assembly an inflammasome, activation caspase-1 and secretion mature inflammasome component, Nalp3, plays non-redundant role response to ATP binding P2X(7) macrophages. Gingival epithelial cells (GECs) are important component the innate-immune...
Abstract Iron overload is associated with various pathological changes which contribute to metabolic syndrome, many of have been proposed occur via damaging tissue through an excessive amount reactive oxygen species (ROS) production. In this study, we established a model iron in L6 skeletal muscle cells and observed that enhanced cytochrome c release from depolarized mitochondria, assayed by immunofluorescent colocalization Tom20 the use JC-1, respectively. This subsequently elevated...
Adiponectin has been shown to mediate cardioprotective effects and levels are typically reduced in patients with cardiometabolic disease. Hence, there intense interest developing adiponectin-based therapeutics. The aim of this translational research study was examine the functional significance targeting adiponectin signaling receptor agonist ALY688 a mouse model heart failure ejection fraction (HFrEF), mechanisms cardiac remodeling leading cardioprotection.
Despite its importance in protecting the host from infections and injury, excessive inflammation may lead to serious human diseases including autoimmune disorders, cardiovascular diseases, diabetes, cancer. Exercise is a known immunomodulator; however, whether exercise causes long-term changes inflammatory responses how these occur are lacking. Here, we show that chronic moderate-intensity training of mice leads persistent metabolic rewiring chromatin accessibility bone marrow-derived...
Abstract Ligation of the purinergic receptor, P2X7R, with its agonist ATP has been previously shown to inhibit intracellular infection by chlamydiae and mycobacteria in macrophages. The effect P2X7R on chlamydial had never investigated preferred target cells chlamydiae, cervical epithelial cells, nor vaginally infected mice. In this study, we show that treatment agonists inhibits partially Chlamydia cells. Chelation magnesium or pretreatment a antagonist blocks inhibitory effects ATP....
Abstract IFNs, which transduce pivotal signals through Stat1 and Stat2, effectively suppress the replication of Legionella pneumophila in primary murine macrophages. Although ability IFN-γ to impede L. growth is fully dependent on Stat1, IFN-αβ unexpectedly suppresses both Stat1- Stat2-deficient New studies demonstrating that robust response lost Stat1-Stat2 double-knockout macrophages suggest Stat2 are functionally redundant their direct an innate toward pneumophila. Because signal...
Iron overload (IO) is associated with cardiovascular diseases, including heart failure. Our study’s aim was to examine the mechanism by which IO triggers cell death in H9c2 cells. caused accumulation of intracellular and mitochondrial iron as shown use iron-binding fluorescent reporters, FerroOrange MitoFerroFluor. Expression cytosolic isoforms Ferritin also induced IO. IO-induced cellular ROS rapid temporally linked. detected cytosol compartments CellROX, DCF-DA MitoSOX dyes partly reversed...
Fibrosis is associated with respiratory and limb muscle atrophy in Duchenne muscular dystrophy (DMD). Current standard of care partially delays the progression this myopathy but there remains an unmet need to develop additional therapies. Adiponectin receptor agonism has emerged as a possible therapeutic target lower inflammation improve metabolism
Abstract Strategies to enhance autophagy flux have been suggested improve outcomes in cardiac ischemic models. We explored the role of adiponectin mediating under conditions induced by permanent coronary artery ligation. studied molecular mechanisms underlying adiponectin's cardio‐protective effects knockout (Ad‐KO) compared with wild‐type (WT) mice subjected ischemia ligation and H9c2 cardiomyocyte cell line exposed hypoxia. Systemic infusion a cathepsin‐B activatable near‐infrared probe as...
The tumor necrosis factor receptor-associated 1 (TRAF1) plays a key role in promoting lymphocyte survival, proliferation, and cytokine production. Recent evidence showed that TRAF1 opposing roles monocytes macrophages where it controls NF-κB activation limits pro-inflammatory production as well inflammasome-dependent IL-1β secretion. Importantly, polymorphisms have been strongly linked to an increased risk of rheumatoid arthritis (RA). However, whether how contributes RA pathogenesis is not...
Abstract Secretion of IL-1β, a potent cytokine that plays key role in gout pathogenesis, is regulated by inflammasomes. TRAF1 has been linked to heightened risk inflammatory arthritis. In this article, we show negatively regulates inflammasome activation limit caspase-1 and IL-1β secretion human mouse macrophages. reduces linear ubiquitination subsequent oligomerization the adapter protein, ASC. i.p. injection monosodium urate crystals resulted increased cell infiltrates production Traf1...