To elucidate the mechanisms underlying maternal undernutrition (MUN)-induced fetal skeletal muscle growth impairment in cattle, longissimus thoracis of Japanese Black calves at 8.5 months utero was analyzed by an integrative approach with metabolomics and transcriptomics. The pregnant cows were fed on 60% (low-nutrition, LN) or 120% (high-nutrition, HN) their overall nutritional requirement during gestation. MUN markedly decreased bodyweight weight fetus. levels amino acids (AAs)...

Abstract This study aimed to investigate the effects of high and low levels energy intake during entire gestation period on skeletal muscle development, organ adipose tissue accumulation in fetuses Wagyu (Japanese Black) cows, a breed with highly marbled beef. Cows were allocated high‐nutrition ( n = 6) group (fed 120% nutritional requirement) or low‐nutrition 60% requirement). The cows artificially inseminated semen from same sire, removed by cesarean section at 260 ± 8.3 days fetal age...

This study aimed to elucidate the effects of maternal undernutrition (MUN) on epigenetic modification hepatic genes in Japanese Black fetal calves during gestation. Using a previously established experimental design feeding dams with 60% (LN) or 120% (HN) their global nutritional requirements 8.5-month gestational period, DNA methylation liver was analyzed reduced representation bisulfite sequencing (RRBS). The promoters and gene bodies LN fetuses were hypomethylated compared HN fetuses....

This study aimed to understand the mechanisms underlying effects of maternal undernutrition (MUN) on liver growth and metabolism in Japanese Black fetal calves (8.5 months utero) using an approach that integrates metabolomics transcriptomics. Dams were fed 60% (low-nutrition; LN) or 120% (high-nutrition; HN) their overall nutritional requirements during gestation. We found MUN markedly decreased body weights fetuses; metabolomic analysis revealed aspartate, glycerol, alanine, gluconate...

We aimed to determine the effects of maternal nutrient restriction (MNR) on DNA methylation and gene expression patterns associated with metabolism immunopoiesis in thymuses fetal Wagyu cattle. Pregnant cows were allocated two groups: a low-nutrition (LN; 60% nutritional requirement;

ABSTRACT The detection and mapping of segregating quantitative trait loci (QTL) that influence withers height, hip width, body length, chest depth, shoulder lumbar thurl pin bone rump cannon circumference, girth, abdominal width girth at weaning was conducted on chromosomal regions bovine chromosome one. QTL analysis performed by genotyping half‐sib progeny five Japanese Black sires using microsatellite DNA markers. Probability coefficients inheriting allele 1 or 2 from the sire specific...

We hypothesized that maternal low or high nutrition would give unique effects to morphological and molecular dynamics in adipose tissue of fetus fatty breed Wagyu (Japanese Black) cattle which produce highly marbled beef. This study aimed determine the energy intake cows, during gestation on fetal development, histochemical properties, gene microRNA (miRNA) expression. Cows were allocated one two nutritional groups: 120% (HIGH) 60% requirements (LOW). Fetuses (n = 6 per treatment) removed...

The development of a sensitive enzymeimmunoassay (EIA) for the determination estrone (E1) and estradiol-17beta (E2 beta) in bovine plasma is described. assay homologous double-antibody EIA with E2beta 17hemisuccinate (HS) as hapten immunoreactive reagent. antiserum was raised against 17HS serum albumin conjugate rabbit, 17HS-horseradish peroxidase used steroid-enzyme conjugate. Each estrogen distinguished only by using each working standard sample EIA. Bovine E1 were extracted purified...

Progesterone levels were determined in the peripheral blood of Japanese monkey during menstrual cycle, breeding season, nonbreeding and gestation. averaged less than 0.3 ng/ml earlier follicular phase, rose to maximum values 3.8 approximately eight days before menses, declined 1.0 menstruation. season comparable with those early phase. In pregnant animals circulating progesterone either continued rise around day 20, or fell as infertile then again. between 35 60 variable until about 130

Mitochondrial DNA haplotypes from the displacement-loop (D-loop) region (436 bp) were genotyped and sequenced in Japanese Black beef cattle raised same herd.Correlation coefficients between mitochondrial haplotypes, maternal lineage, birth weight, preweaning average daily gain, weaning post gain yearling weight computed.The objective was to study relationship postnatal growth traits investigate if of calves age could be accurately predicted haplotypes.Results phylogenetic analysis revealed...

Aim: This study was conducted to examine the effect of blood metabolites on embryo quality in post-partum suckling Japanese Black cattle. Methods: Blood samples were taken from 23 cows 30 days before, at and after parturition. Cows synchronized 40 or 41 calving (day 0) divided into three groups: control (n = 6), gonadotropin-releasing hormone ([GnRH]n 10) estradiol benzoate ([EB]n 7). All groups received a controlled internal drug release (CIDR) device intravaginally together with 2 mg EB...

Bovine chromosomes 2 (BTA2) and 5 (BTA5) of purebred, half-sib progeny sired by five Japanese black bulls were genotyped using microsatellite DNA markers. The data subjected to linkage analysis for the detection mapping segregating quantitative trait loci (QTL) influencing live weight, average daily gain body measurements at weaning. Probability coefficients inheriting allele 1 or from sire specific chromosomal intervals computed. phenotypic on regressed these probability in a...

ABSTRACT A confirmatory scan for the regions of bovine chromosome 1 segregating quantitative trait loci (QTL) influencing birthweight, weaning weight, yearling and preweaning postweaning average daily gains was performed by genotyping half‐sib progeny four Japanese Black sires using microsatellite DNA markers. Data were analyzed generating an F ‐statistic every cM on a linkage map regression phenotype probabilities inheriting allele from sire after adjusting fixed effects sire, sex, parity...

Macrophage colony-stimulating factor (M-CSF) is a hemopoietic cytokine with primary role in placental physiology. Gene expression of M-CSF the bovine endometrium shows temporal upward trend during early and mid pregnancy. This study determined plasma levels pregnancy using ELISA. In experiment 1, to investigate relationship between concentration peripheral blood pregnancy, were 125 pregnant 21 non-pregnant Japanese Black cows. The animals divided into nine groups based on month An ELISA for...

The reproductive performance of postpartum cows is affected by factors such as suckling and nutrition. We investigated the effect a restricted period on superovulatory response fertility after flushing in Japanese Black cows. Forty-seven were used this study. At 7 days postpartum, divided into 2 groups: (1) continuous access to calves from birth weaning at 3 months (ad libitum group; n=20); (2) twice daily penned adjacent them (restricted n=27). All initiated superstimulatory treatment with...

The availability of highly polymorphic microsatellite DNA markers on genetic maps in different livestock species and their association with phenotypes now gives geneticists breeders an effective tool for the detection quantitative trait loci (QTL) affecting traits interest. This paper reviews QTL methodology emphasis multi-point interval mapping half-sib populations. It also summarises published reports QTLs detected by researchers bovine genome suggests way forward Japanese Black cattle.

Journal Article Rapid communication: nucleotide sequence of the bovine elongation factor 1 alpha cDNA1 Get access T Kojima, Kojima *Department Livestock and Grassland Science, National Agricultural Research Center for Western Region, Oda, Japan. Search other works by this author on: Oxford Academic PubMed Google Scholar K Oshima, Oshima H Watanabe, Watanabe M Komatsu 2Correspondence: phone: 81-854-82-1285; fax: 81-854-82-2280; Email: mkomatsu@naro.affrc.go.jp. Animal Volume 80, Issue 6, June...