A5102797183- Meat and Animal Product Quality
- Muscle Physiology and Disorders
- Animal Nutrition and Physiology
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Cell death mechanisms and regulation
- Biochemical effects in animals
- Biochemical Analysis and Sensing Techniques
- RNA Interference and Gene Delivery
- Rice Cultivation and Yield Improvement
- GABA and Rice Research
- Exercise and Physiological Responses
- Cellular transport and secretion
- Agriculture, Soil, Plant Science
- MicroRNA in disease regulation
- Electromagnetic Fields and Biological Effects
- Retinal Development and Disorders
- Congenital heart defects research
- Cancer-related molecular mechanisms research
- Advanced Scientific Research Methods
- Circular RNAs in diseases
- Thermoregulation and physiological responses
- Genomics, phytochemicals, and oxidative stress
- Muscle activation and electromyography studies
- Calpain Protease Function and Regulation
Nippon Veterinary and Life Science University
2018-2024
Western Region Agricultural Research Center
2006-2019
Kagoshima University
2016-2018
Johns Hopkins Medicine
2018
Johns Hopkins University
2018
National Agriculture and Food Research Organization
2014
Niigata University
2009-2013
Engineering Systems (United States)
2011-2012
NGK Spark Plug (Japan)
2009
Osaka University
1998-2007
The objective of this study was to investigate the differences in muscle proteome grass-fed and grain-fed cattle. Eight Japanese Black Cattle 10 mo age were separated randomly into 2 groups: 1) grazing (grass-fed) 2) concentrate (grain-fed) groups. All cattle first housed individually a stall barn fed combination ad libitum Italian ryegrass hay until 21 age. After control period, 4 placed on outdoor pasture, whereas other continued diet. slaughtered at 27 age, tissues from semitendinosus...
MicroRNA (miRNA) are highly conserved, noncoding small RNA involved in post-transcriptional gene regulation a variety of biological processes. To elucidate roles miRNA bovine muscle type specification and maintenance, we sought to determine differentially expressed between semitendinosus (STD) masseter (MS) muscles from 3 Japanese black cattle by massively parallel sequencing. Differential expression myosin heavy chain (MyHC) isoforms confirmed that STD MS were MyHC-2x- MyHC-1-abundant...
We investigated the acute effect of static magnetic fields up to 8 T on skin blood flow and body temperature in anesthetized rats. These variables were measured prior to, during, following exposure a field superconducting magnet with horizontal bore. The dorsal was transversely incised for 1 cm make subcutaneous pocket. Probes laser Doppler flowmeter thermistor inserted into pocket positioned at mid-dorsum measure temperature. Another probe put rectum monitor rectal After baseline...
In addition to the caspase family of proteinases, cathepsin D, a lysosomal aspartic proteinase, has been suggested act as proapoptotic mediator in mammalian cells. To further understand roles cathepsins B and D apoptosis cells, we examined precise alteration processes ultrastructures immunoreactivity for these enzymes PC12 cells cultured under serum deprivation.Laser scanning microscopy showed be finely distributed cytoplasm at onset culture deprivation. At 3h after culture, slightly...
Abstract In fish, the pectoral appendage is adjacent to head, but during vertebrate evolution a long neck region emerged via caudal relocation of appendage. The comprised endochondral portions, such as humerus and scapula, dermal portion, clavicle, that contributes shoulder girdle. search for clues mechanism appendage, cell lineage rostral lateral plate mesoderm was analyzed in chickens. It found that, despite chickens, origin clavicle attached head ranged between 1 14 somite levels. Because...
We aimed to understand the roles of miRNAs in muscle tissue maturation and those circulating microRNAs (c-miRNAs) beef production Japanese Black (JB) cattle (Wagyu), a breed with genetically background superior intermuscular fat depot, by comparing different feeding conditions (indoor grain-feeding vs. grazing on pasture). The at 18 months old were assigned pasture or conventional indoor grain for 5 months. Microarray analysis c-miRNAs from plasma extracellular vesicles led detection total...
Abstract Taste is a crucial factor of meat quality, and amino acids are important taste‐active components in meat. Here, the effects dietary lysine (Lys) content on meat, especially free glutamate (Glu), were investigated. Twenty‐eight‐day‐old broilers ( Gallus gallus ) fed diets with graded Lys 90% or 100% recommended requirement, (according to National Research Council, for 10 days. Free acid sensory scores soup estimated. Glu content, main component was significantly increased by...
Taste is crucial to meat quality, and free Glu an important taste-active component in meat. Our recent study showed that the short-term feeding of a low-Lys diet increases concentration other amino acids chicken muscle improves its taste. Here, we investigated mechanisms by which muscle. Two groups (n = 10 per group) 28-day-old female Ross strain broiler chickens were fed diets with graded Lys content 90% or 100% recommended requirement (according National Research Council [1994] guidelines)...
Myokines are muscle-secreted factors to regulate cellular functions. However, it remains elusive what type of myokine is released during muscle differentiation. Here we evaluated the dynamics myokines. More than 400 proteins were detected in conditioned medium and approximately 8% them categorized as The levels myokines which promote myotube formation, vascularization or neurogenesis peaked early differentiation, whereas contributing repellent activity against nerve cells suppression...
Myostatin is a specific negative regulator of skeletal muscle growth and regarded as one the most important factors for myogenesis. The aim current study was to analyze developmental change in gene expression myostatin an adipogenic transcription factor (peroxisome proliferator-activated receptor lambda2) semitendinosus Japanese Black Cattle throughout whole life cycle. An additional compare temporal patterns relevant myogenic regulatory (MRF) mRNA during regeneration after frostbite injury...
ABSTRACT To assess the role of muscle fiber type in beef taste‐traits, we analyzed cooked meats from bovine masseter, diaphragm, psoas major, longissimus thoracis, and semitendinosus muscles with an electric taste sensing system (INSENT SA402B). The is composed five sensors polymer membranes fixing different lipids. sensors, CT0, CA0, AAE, C00 AE1 are designed to respond individual tastes salty, sour, umami, bitter astringent, respectively. found significant differences converted outputs CA0...
ABSTRACT Messenger RNA (mRNA) expression of calpain‐1 (µ‐calpain), ‐2 (m‐calpain), ‐3 (p94), small subunit (calpain‐4; 28 kDa), and three types calpastatin (CSTN) isoform were investigated for 10 skeletal muscles Holstein cattle by real‐time and/or semi‐quantitative reverse transcription polymerase chain reaction. Noticeably, effect muscle type was observed on kDa ( P < 0.001) with a tendency higher in myosin heavy (MyHC)‐2x‐rich compared to MyHC‐slow‐rich muscles. The CSTN‐I –III...
Deoxyribonuclease II (DNase II) is a key enzyme in the phagocytic digestion of DNA from apoptotic nuclei. To understand molecular properties DNase II, particularly processing, we prepared polyclonal antibody against carboxyl-terminal sequences mouse II. In present study, partial purification using Con A Sepharose enabled detection endogenous by Western blotting. It was interesting that two forms were detected – 30 kDa form and 23 form. Neither those carried expected weight 45 kDa....
Abstract Regulation of taste is important for improving meat quality and glutamate ( G lu) one the taste‐active components in meat. Here, effects dietary lysine L ys) content on meat, especially free Glu, were investigated. Fourteen‐day‐old broiler chicks allus gallus ) fed diets containing 100% or 150% recommended ys 10 days. Concentrations amino acids plasma, muscle liver measured. The levels messenger RNAs mRNAs enzymes related to lu metabolism determined liver. concentration metabolites...
ABSTRACT Experiments were designed to compare the adipocyte cellularity of subcutaneous adipose tissue between growing Landrace (low backfat) and Meishan (high pigs at 1 week, 3 weeks, 6 months 5 age. As aged, body weight backfat thickness both breeds significantly increased. When compared equal ages, adjusted was greater for pigs. The mean diameter fat cell size also increased with age, by weeks adipocytes from outer inner layers larger in At months, approximately 80% mass attributable...
ABSTRACT The composition of tropomyosin (TPM) and myosin heavy chain (MyHC) isoforms was analyzed in 10 physiologically different bovine muscles ( masseter , diaphragm, tongue, semispinalis, pectoralis profundus biceps femoris, psoas major semimembranosus, longissimus thoracis semitendinosus ) to clarify the relationships between TPM MyHC muscle fiber types. content TPM1 TPM3 according their function contraction, although TPM2 constantly about 50% total all muscles. higher semimembranosus...
We divided the developmental processes from microspore differentiation to fertilization into 4 phases and defined component relating in each phase. The percentage of was expressed by product components which are number differentiated microspores (X1), developed pollen grains (X2), shed (X3) efficiency (X4). There were greate varietal differences contribution degrees these fertilization. ratio estimated multiple regression analysis, using 19 rice varieties differing cooling tolerance. first 3...
Abstract In skeletal muscle cells, myofibrillar proteins are highly organized into sarcomeres in which thick filaments interdigitate with thin to generate contractile force. The size of filaments, consist mainly myosin molecules, is strictly controlled. However, little known about the mechanisms by molecules assemble filaments. Here, we assessed ability each domain heavy chain (Myh) form We showed that exogenously expressed subfragment 2 ( S2 ) + light meromyosin LMM Myh was efficiently...