A5089324899- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Diet, Metabolism, and Disease
- Neuroendocrine regulation and behavior
- Immune Cell Function and Interaction
- Neuropeptides and Animal Physiology
- Glioma Diagnosis and Treatment
- Genetics, Aging, and Longevity in Model Organisms
- Cancer, Hypoxia, and Metabolism
- Acute Ischemic Stroke Management
- Endoplasmic Reticulum Stress and Disease
- Dermatological diseases and infestations
- Urticaria and Related Conditions
- Electrolyte and hormonal disorders
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Sarcoma Diagnosis and Treatment
- Cerebrovascular and Carotid Artery Diseases
- Spinal Hematomas and Complications
- Case Reports on Hematomas
- Analytical Chemistry and Chromatography
- Hedgehog Signaling Pathway Studies
- Peroxisome Proliferator-Activated Receptors
Hamanomachi Hospital
2025
Osaka University
1989-2021
Institute of Livestock and Grassland Science
2003-2017
National Agriculture and Food Research Organization
2017
Mitsubishi Tanabe Pharma Corporation
2010-2016
National Institute of Biomedical Innovation, Health and Nutrition
2014-2016
Nagoya University
1964-2015
Mitsubishi Chemical (Japan)
2010-2015
Institut de Recherche Vaccinale
2014
Kyushu University
2008-2013
Significance CpG oligodeoxynucleotide (ODN), a Toll-like receptor 9 ligand, is promising immunotherapeutic agent; however, developing an IFN-inducing ODN forming stable nanoparticle without aggregation has been unsuccessful. Here we generated nanoparticulate (K3) wrapped by the nonagonistic Dectin-1 ligand schizophyllan (SPG), K3-SPG. K3-SPG stimulates human peripheral blood mononuclear cells to produce large amounts of both type I and II IFN. thus became potent adjuvant, especially for...
Neuropeptide Y (NPY) in the arcuate nucleus is an orexigenic hormone of which levels are regulated by humoral as well neural signals. In this study, we examined regulation NPY gene expression hypothalamic organotypic cultures. Dexamethasone (DEX) (10 –9 to 10 –7 m ) significantly increased mRNA expression, and effects were not influenced coincubation with sodium channel blocker tetrodotoxin (TTX), indicating that action DEX independent potentials. Conversely, insulin –11 inhibited stimulated...
Ghrelin, which was identified from the rat stomach, is a potent stimulant for food intake. Several lines of evidence suggest that orexigenic action ghrelin mediated via neuropeptide Y (NPY) neurons in arcuate nucleus, although detailed mechanisms by stimulates NPY are not clear. In this study, we examined gene regulation and agouti-related peptide (AGRP), another synthesized neurons, nucleus hypothalamic organotypic cultures. Incubation explants with significantly increased AGRP mRNA...
Advax, a delta inulin-derived microparticle, has been developed as an adjuvant for several vaccines. However, its immunological characteristics and potential mechanism of action are yet to be elucidated. Here, we show that Advax behaves type-2 when combined with influenza split vaccine, T helper (Th)2-type antigen, but type-1 inactivated whole virion (WV), Th1-type antigen. In addition, effect was not observed Advax-adjuvanted WV vaccine used immunize toll-like receptor (TLR) 7 knockout mice...
Familial neurohypophysial diabetes insipidus (FNDI), an autosomal dominant disorder, is mostly caused by mutations in the gene of neurophysin II (NPII), carrier protein arginine vasopressin (AVP). Previous studies suggest that loss AVP neurons might be cause polyuria FNDI. Here we analyzed knockin mice expressing mutant NPII causes FNDI humans. The heterozygous manifested progressive as do patients with Immunohistochemical analyses revealed inclusion bodies were not immunostained antibodies...
Abstract Umbilical cord blood transplantation (CBT) is accepted as an effective treatment for acute myeloid leukemia (AML), and reduced-intensity conditioning (RIC), rather than myeloablative (MAC) regimens allowed elderly patients to be treated safely. However, appropriate intensities of are still unclear, especially middle-aged patients. To compare outcomes after RIC MAC regimens, we analyzed AML aged 16 years or older in the Japanese registry database, who underwent single unit CBT...
We aimed to understand the roles of miRNAs in muscle tissue maturation and those circulating microRNAs (c-miRNAs) beef production Japanese Black (JB) cattle (Wagyu), a breed with genetically background superior intermuscular fat depot, by comparing different feeding conditions (indoor grain-feeding vs. grazing on pasture). The at 18 months old were assigned pasture or conventional indoor grain for 5 months. Microarray analysis c-miRNAs from plasma extracellular vesicles led detection total...
// Yuji Kitahata 1,2 , Tomohiro Kanuma 1,3 Masayuki Hayashi 1 Nobuyoshi Kobayashi Koji Ozasa 1,5 Takato Kusakabe Burcu Temizoz 3 Etsushi Kuroda Hiroki Yamaue 2 Cevayir Coban 4 Takuya Yamamoto Kouji Kobiyama Taiki Aoshi and Ken J. Ishii Labotatory of Adjuvant Innovation, National Institutes Biomedical Health Nutrition (NIBIOHN), Osaka, Japan 2nd Department Surgery, Wakayama Medical University, Wakayama, Laboratory Vaccine Science, WPI Immunology Frontier Research Center (IFReC), Osaka Malaria...
Abstract Altered contractility of airway smooth muscle (SM) is one the main causes allergic asthma, in which predominance Th2 over Th1 cytokines plays a central role. In present study, we examine effects on SM contraction. Treatment with low concentration IL-4 (0.2 ng/ml) for 6 h augmented, whereas higher concentrations (2–20 inhibited, agonist-induced contractions collagen gels containing bovine tracheal cells. Another cytokine (IL-13) showed an augmentation gel contraction range 20–200...
Peripheral administration of baclofen significantly reduced food intake and body weight increase in both diabetic (db/db) diet-induced obese mice for 5 weeks, whereas it had no significant effects on energy balance their lean control mice. Despite the decreased weight, neuropeptide Y expression arcuate nucleus was decreased, pro-opiomelanocortin increased by treatment. These data demonstrate that inhibitory were mediated via at least partially, suggest GABA(B) agonists could be a new...
Familial neurohypophysial diabetes insipidus (FNDI) is caused by mutations in the gene locus of arginine vasopressin (AVP), an antidiuretic hormone. Although carriers are normal at birth, polyuria and polydipsia appear several months or years later. Previously, we made mice possessing a mutation causing FNDI reported that manifested progressive as do patients with FNDI. Here, report decreases AVP mRNA expression supraoptic nucleus were accompanied shortening poly(A) tail length mice, case...
A simple surgical technique for correcting posttraumatic enophthalmos is described. The steps are as follows: (1) a plaster mold obtained of the patient's face, (2) wax added to enophthalmic eye until it becomes symmetrical, (3) quantity measured, and (4) same amount sliced costal cartilage implanted beneath periosteum extended orbital wall behind vertical axis globe. Using this technique, we have successfully treated six patients with traumatic floor defects without complication. This...
In this study, we examined the effects of intracerebroventricular administration melanotan II (MTII), a melanocortin agonist, on insulin sensitivity in diet-induced obese (DIO) rats. Although MTII treatment significantly decreased food intake and body weight for 10 days, there was no significant difference between pair-fed groups. The tolerance test showed that improved group compared to group. Furthermore, increased number small-sized adipocytes epididymal white adipose tissues, suggesting...
Familial neurohypophysial diabetes insipidus (FNDI) is a rare disease that inherited in an autosomal dominant manner. In previous study, we made mouse model for FNDI, which showed progressive polyuria accompanied by inclusion bodies the arginine vasopressin (AVP) neurons formed aggregates endoplasmic reticulum. The present study was conducted to determine whether activities of AVP are related phenotype progression FNDI model. first experiment, female heterozygous mice were administered...
A modified oligodeoxynucleotide (ODN) containing N(3)-methyl-5-cyanovinyl-2'-deoxyuridine reacts by photoirradiation at 366 nm with an adenine residue of a complementary template ODN to yield end-capped in 87% yield.