A5017619968- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Influenza Virus Research Studies
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- Viral Infections and Vectors
- Infrared Thermography in Medicine
- Lymphoma Diagnosis and Treatment
- Virology and Viral Diseases
- Human-Animal Interaction Studies
- Viral Infections and Immunology Research
- Biosensors and Analytical Detection
- Respiratory viral infections research
- COVID-19 epidemiological studies
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
Agriculture Fisheries and Conservation Department
2020-2024
University of Hong Kong
2020-2022
Food and Health Bureau
2022
University of Liège
2020
City University of Hong Kong
2020
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first detected in Wuhan December 2019 and caused disease (COVID-19)1,2. In 2003, the closely related SARS-CoV had been domestic cats a dog3. However, little is known about susceptibility of pet mammals to SARS-CoV-2. Here, using PCR with reverse transcription, serology, sequencing viral genome virus isolation, we show that out 15 dogs from households confirmed human cases COVID-19 Hong Kong were found be infected SARS-CoV-2 RNA...
Abstract We tested 50 cats from coronavirus disease households or close contacts in Hong Kong, China, for severe acute respiratory syndrome 2 RNA and fecal samples. found 6 cases of apparent human-to-feline transmission involving healthy cats. Virus genomes sequenced 1 cat its owner were identical.
Surrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus test (sVNT) comparison to 90% plaque reduction tests (PRNT 90 ) human, canine, cat, hamster sera. With PRNT as the reference, sVNT had sensitivity of 98.9% specificity 98.8%. Using panel immune sera corresponding other coronaviruses, we confirm lack...
Lumpy skin disease virus (LSDV) is an emerging poxviral pathogen of cattle that currently spreading throughout Asia. The situation high importance for farmers and policy makers in In October 2020, feral Hong Kong developed multi-focal cutaneous nodules consistent with lumpy (LSD). Gross histological pathology further supported the diagnosis samples were sent to OIE Reference Laboratory at Pirbright Institute confirmatory testing. LSDV was detected using quantitative polymerase chain reaction...
We isolated three genotypes of highly pathogenic avian influenza virus (HPAIV) clade 2.3.4.4b from wild birds infected with H5N1 (n = 12) and H5N8 1) in Hong Kong SAR 2021–2024. Viruses two spoonbills late 2022 were genetically related to a human China. Four tested viruses exhibited variable virulence mice but susceptible approved antivirals. No neutralising antibody was detected 63 age-stratified sera, suggesting potential risk should the adapt humans.
Zoonotic and pandemic influenza continue to pose threats global public health. Pandemics arise when novel A viruses, derived in whole or part from animal avian adapt transmit efficiently a human population that has little immunity contain its onward transmission. Viruses of previous concern, such as A(H7N9), arose A(H9N2) viruses established domestic poultry acquiring hemagglutinin neuraminidase aquatic waterfowl. We report A(H3N8) virus chicken emerged similar manner been recently reported...
Background: Transmission of SARS-CoV-2 from humans to other mammals, including pet animals, has been reported. However, with the exception farmed mink, there is no previous documentation that these infected animals can infect humans, nor further onward spread among humans. Following a confirmed infection store worker, in and warehouse supplying it were tested for evidence infection.Methods: Viral swabs blood samples collected shop by RT-PCR serological assays, respectively. positive studied...
The global spread of the A/goose/Guangdong/1/96-lineage H5N1 highly pathogenic avian influenza (HPAI) viruses is accompanied by an expanded host range and establishment sustained viral transmission among dairy cattle. To evaluate if evolving have changed tissue tropism over time, we compared binding patterns recombinant hemagglutinin (HA) proteins derived from clade 1 (A/Vietnam/1203/04, H5VN) circulating 2.3.4.4b detected a wild bird (A/Eurasian Teal/Hong Kong/AFCD-HKU-23-14009-01020/2023,...
Loop-mediated isothermal amplification (LAMP) has received considerable attention for decentralized (point-of-care and on-site) nucleic acid testing in view of its simple temperature control (60-65 °C) short assay time (15-60 min). There remains a challenge wide adoption acceptance due to the limitations existing result reporter probes, e.g., photobleaching organic fluorophore reduced sensitivity pH-sensitive colorimetric dye. Herein, we demonstrate CdSeS/ZnS quantum dots (semiconductor...
In this study, we describe the epidemiological investigation of first African swine fever (ASF) outbreak in a local domestic pig farm New Territories Hong Kong 2021. farm, several affected pigs presented clinical and pathological signs consistent with ASF, while remaining showed nonspecific or did not exhibit any signs. The relative low morbidity mortality ASF on resulted delayed detection implementation control response. Despite delay, no further spread disease from to other farms wild...
Abstract Surrogate neutralization assays for SARS-CoV-2 that can be done without biosafety-level-3 containment and across multiple species are desirable. We evaluate a recently developed surrogate virus test (sVNT) in comparison to 90% plaque reduction tests (PRNT90) human, canine, cat hamster sera found excellent concordance between the two assays. Using panel of immune other coronaviruses, we confirm lack cross reactivity sVNT PRNT90
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide since its emergence in 2019. Knowing the potential capacity virus to adapt other species, serological surveillance SARS-CoV-2 infection susceptible animals is important. Hong Kong and Seoul are two Asia’s most densely populated urban cities, where companion often live close contact with humans. Sera collected from 1040 cats 855 dogs during early phase pandemic were tested for antibodies...
Summary Lumpy skin disease virus (LSDV) is an emerging poxviral pathogen of cattle that currently spreading throughout Asia. The situation high importance for farmers and policy makers in In October 2020, feral Hong Kong developed multifocal cutaneous nodules consistent with lumpy (LSD). Gross histological pathology further supported the diagnosis samples were sent to OIE Reference Laboratory at Pirbright Institute confirmatory testing. LSDV was detected using quantitative polymerase chain...
Abstract SARS-CoV-2 emerged in Wuhan December 2019 and has caused the pandemic respiratory disease, COVID-19. Following what is presumed to be an initial zoonotic transmission event, virus now spreading efficient humans. Very little known about susceptibility of domestic mammals kept as pets this virus. Samples were collected over a 13-day period from 17 year-old neutered male Pomeranian Hong Kong SA that was taken into isolation after two members household tested positive for Nasal swabs...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide since its emergence in 2019. The current global pandemic was driven by human-to-human transmission. Knowing the zoonotic origin disease and potential capacity virus to adapt other species, it is important understand extent natural SARS-CoV-2 infection animals, particular cats dogs households that are direct contact with their owners. Hong Kong Seoul two most densely-populated urban cities...
Abstract Wild waterfowl serve as a reservoir of some astroviruses. Fecal samples from wild collected at Hong Kong's Marshes were tested using pan-astrovirus reverse transcription-PCR. Positive underwent subsequent host identification DNA barcoding. Based on deduced partial sequences, noteworthy three astrovirus groups (mammalian, avian and unclassified astroviruses) further analyzed by next-generation sequencing. One sample Avastrovirus 4 clade, MP22-196, had nearly complete genome...
Literature covering diseases of the giant panda ( Ailuropoda melanoleuca) is either in Chinese or focuses on infectious agents. Here we describe clinical signs, gross and microscopic findings, immunohistochemistry results a B-cell lymphoma multiple organs 35-y-old captive male panda. The animal was euthanized because prolonged anorexia vomiting. Postmortem examination revealed ascites, generalized lymphadenopathy, splenomegaly, hepatomegaly, thickened gall bladder mucosa. Histologically,...