A5047221605- Cellular Mechanics and Interactions
- Nuclear Structure and Function
- RNA Research and Splicing
- Force Microscopy Techniques and Applications
- Microstructure and mechanical properties
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Metal and Thin Film Mechanics
- RNA regulation and disease
- Nanofabrication and Lithography Techniques
- Aluminum Alloys Composites Properties
- Lipid Membrane Structure and Behavior
- Biochemical and Structural Characterization
- Advanced Memory and Neural Computing
- Genomics and Chromatin Dynamics
- Polymer Surface Interaction Studies
- Advanced Electron Microscopy Techniques and Applications
- Caveolin-1 and cellular processes
- Cardiac electrophysiology and arrhythmias
- Microfluidic and Bio-sensing Technologies
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Metal Forming Simulation Techniques
- DNA Repair Mechanisms
University of California, San Diego
2021-2025
La Jolla Bioengineering Institute
2024-2025
Jacobs (United States)
2021-2025
Stanford University
2020-2024
University of California System
2021-2024
UC San Diego Health System
2024
Neurosciences Institute
2022
University of California, Berkeley
2013-2020
Multidisciplinary Digital Publishing Institute (Switzerland)
2018
University of Waterloo
2011-2018
Both substrate stiffness and surface topography regulate cell behavior through mechanotransduction signaling pathways. Such intertwined effects suggest that engineered topographies might substitute or cancel the of in biomedical applications. However, mechanisms by which cells recognize topographical features are not fully understood. Here we demonstrate presence nanotopography drastically alters such neurons stem cultured on rigid glass substrates behave as if they were soft hydrogels. With...
Abstract Drug-induced cardiotoxicity arises primarily when a compound alters the electrophysiological properties of cardiomyocytes. Features intracellular action potentials (iAPs) are powerful biomarkers that predict proarrhythmic risks. In last decade, number vertical nanoelectrodes have been demonstrated to achieve parallel and minimally-invasive iAP recordings. However, large variability in success rate signal strength hindered from being broadly adopted for proarrhythmia drug assessment....
Abstract Integrin-mediated focal adhesions are the primary architectures that transmit forces between extracellular matrix (ECM) and actin cytoskeleton. Although abundant on rigid flat substrates support high mechanical tensions, they sparse in soft three-dimensional (3D) environments. Here we report curvature-dependent integrin-mediated called curved adhesions. Their formation is regulated by membrane curvatures imposed topography of ECM protein fibres. Curved mediated integrin ɑvβ5...
Abstract Objectives To identify landmarks in ultrasound periodontal images and automate the image-based measurements of gingival recession (iGR), height (iGH), alveolar bone level (iABL) using machine learning. Methods We imaged 184 teeth from 29 human subjects. The dataset included 1580 frames for training validating U-Net CNN learning model, 250 new that were not used testing generalization performance. predicted including tooth, gingiva, bone, margin (GM), cementoenamel junction (CEJ),...
The development of biomaterials capable regulating cellular processes and guiding cell fate decisions has broad implications in tissue engineering, regenerative medicine, cell-based assays for drug disease modeling. Recent studies have shown that three-dimensional (3D) nanoscale physical cues such as nanotopography can modulate various like adhesion endocytosis by inducing curvature on the plasma nuclear membranes. Two-dimensional (2D) biochemical protein micropatterns also regulate function...
Single-wall carbon nanotubes (SWCNTs) have been widely used for biological applications in recent years, and thus, it is critical to understand how these inert nanomaterials influence cell behavior. Recently, has observed that cellular phenotypes such as proliferation, force generation growth change upon SWCNT treatment, SWCNTs directly affect the organization redistribution of actin cytoskeleton. However, interactions between at molecular level or this interaction changes structure remain...
Bacteria have evolved as intelligent microorganisms that can colonize and form highly structured cooperative multicellular communities with sophisticated singular collective behaviors. The initial stages of colony formation intercellular communication are particularly important to understand depend on the spatial organization cells. Controlling distribution growth bacterial cells at nanoscale is, therefore, great interest in understanding mechanisms cell–cell formation. Staphyloccocus...
Abstract Intracellular electrophysiology is essential in neuroscience, cardiology, and pharmacology for studying cells’ electrical properties. Traditional methods like patch-clamp are precise but low-throughput invasive. Nanoelectrode Arrays (NEAs) offer a promising alternative by enabling simultaneous intracellular extracellular action potential (iAP eAP) recordings with high throughput. However, accessing potentials NEAs remains challenging. This study presents an AI-supported technique...
TAR DNA-binding protein (TDP-43) and Metastasis Associated Lung Adenocarcinoma Transcript (MALAT1) RNA are both abundantly expressed in the human cell nucleus. Increased interaction of TDP-43 MALAT1, as well dysregulation function, was previously identified brain samples from patients with neurodegenerative disease compared to healthy tissues. We hypothesized that function may depend part on MALAT1 expression levels. Here, we find alterations affect viability can modulate binding other mRNAs...
Contact sites between the endoplasmic reticulum (ER) and plasma membrane (PM) play a crucial role in governing calcium regulation lipid homeostasis. Despite their significance, factors regulating spatial distribution on PM remain elusive. Inspired by observations cardiomyocytes, where ER-PM contact concentrate tubular invaginations known as transverse tubules, we hypothesize that curvature plays formation. Through precise control of invaginations, show curvatures locally induce formation...
Linkers of the nucleoskeleton and cytoskeleton are key molecular complexes that span nuclear envelope (NE) provide a direct linkage between cytoskeleton. Two major components these members SUN KASH protein families interact in perinuclear space to allow transmission mechanochemical signals across NE. Structural details mammalian domain SUN2 have established must form trimer bind KASH, this trimerization is mediated through two predicted coiled-coil regions protein, CC1 CC2, which precede...