A5062078840- Thyroid Cancer Diagnosis and Treatment
- BRCA gene mutations in cancer
- Renal and related cancers
- Melanoma and MAPK Pathways
- Cancer-related Molecular Pathways
- Multiple and Secondary Primary Cancers
- Renal cell carcinoma treatment
- Genetic factors in colorectal cancer
- Cutaneous Melanoma Detection and Management
- Connective Tissue Growth Factor Research
- Epigenetics and DNA Methylation
- Ovarian cancer diagnosis and treatment
- S100 Proteins and Annexins
- Brain Metastases and Treatment
- Galectins and Cancer Biology
- Cancer Mechanisms and Therapy
- RNA modifications and cancer
- Viral-associated cancers and disorders
- Clusterin in disease pathology
- Genetic Associations and Epidemiology
- Head and Neck Anomalies
- Hedgehog Signaling Pathway Studies
- Ferroptosis and cancer prognosis
- Lung Cancer Research Studies
- Thyroid and Parathyroid Surgery
The Maria Sklodowska-Curie National Research Institute of Oncology
2009-2024
Silesian University of Technology
2013-2017
Giessen School of Theology
1997
Justus-Liebig-Universität Gießen
1997
Infinium HumanMethylation 450 BeadChip Arrays by Illumina (Illumina HM450K) are among the most popular CpG microarray platforms widely used in biological and medical research. Several recent studies highlighted potentially confounding impact of genomic variation on results methylation performed using Illumina's probes. However, complexity SNPs level measurements (β values) has not been comprehensively described. In our comparative study European Asian populations HM450K, we found that...
The molecular etiology of follicular thyroid tumors is largely unknown, rendering the diagnostics these challenging. somatic alterations present in apart from RAS gene mutations and PAX8/PPARG translocations are not well described. To evaluate profile alteration tumors, a total 82 tissue samples derived 48 patients were subjected to targeted Illumina HiSeq next generation sequencing 372 cancer-related genes. New identified oncogenes (MDM2, FLI1), transcription factors repressors (MITF, FLI1,...
TERT promoter (TERTp) mutations are important factors in papillary thyroid carcinomas (PTCs). They associated with tumor aggressiveness, recurrence, and disease-specific mortality their use risk stratification of PTC patients has been proposed. In this study we investigated the prevalence TERTp a cohort Polish PTCs association these histopathological factors, particularly coexistence BRAF V600E mutation. A total 189 consecutive specimens known mutational status were evaluated. detected 8.5%...
Abstract Background Differential diagnosis between malignant follicular thyroid cancer (FTC) and benign adenoma (FTA) is a great challenge for even an experienced pathologist requires special effort. Molecular markers may potentially support differential FTC FTA in postoperative specimens. The purpose of this study was to derive molecular post-operative diagnosis, the form simple multigene mRNA-based classifier that would differentiate tissue samples. Methods A created based on combined...
Papillary thyroid carcinoma (PTC) is most common among all cancers. Multiple genomic alterations occur in PTC, and gene rearrangements are one of them. Here we screened 14 tumors for novel fusion transcripts by RNA‐Seq. Two samples harboring RET/PTC1 RET/PTC3 were positive controls whereas the remaining ones negative regarding PTC alterations. We used Sanger sequencing to validate potential fusions. detected 2 potentially oncogenic transcript fusions: TG‐FGFR1 TRIM33‐NTRK1 . 4 unknown...
Pilocytic astrocytoma is the most common type of brain tumor in pediatric population, with a generally favorable prognosis, although recurrences or leptomeningeal dissemination are sometimes also observed. For tumors originating supra-or infratentorial location, different molecular background was suggested, but plausible correlations between transcriptional profile and radiological features and/or clinical course still undefined. The purpose this study to identify gene expression profiles...
Molecularly targeted therapy has revolutionized the treatment of advanced melanoma. However, despite its high efficiency, a majority patients experience relapse within 1 year because acquired resistance, and approximately 10-25% gain no benefit from these agents owing to intrinsic resistance. This is mainly caused by genetic heterogeneity melanoma cells.We aimed validate predictive significance selected genes in before with BRAF/MEK inhibitors.Archival DNA derived 37 formalin-fixed...
Background: Telomerase reverse transcriptase promoter (TERTp) mutations are related to a worse prognosis in various malignancies, including papillary thyroid carcinoma (PTC). Since mechanisms responsible for the poorer outcome of TERTp(+) patients still unknown, searching molecular consequences TERTp PTC was aim our study. Methods: The studied cohort consisted 54 PTCs, among them 24 cases with distant metastases. BRAF V600E, RAS, and mutational status evaluated all cases. Differences gene...
The molecular mechanisms of cell cycle exit are poorly understood. Studies on lymphocytes at after growth factor deprivation have predominantly focused the initiation apoptosis. We aimed to study gene expression profile primary and immortalised IL-2-dependent human T cells forced by withdrawal, before apoptosis could be evidenced. By Affymetrix microarrays HG-U133 2.0 Plus, 53 genes were distinguished as differentially expressed soon IL-2 deprivation. Among those, PIM1, BCL2, IL-8, HBEGF,...
Introduction: Somatic RET mutations are detectable in two-thirds of sporadic cases medullary thyroid cancer (MTC). Recent studies reported a high proportion RAS somatic negative tumours, which may indicate mutation as possible alternative genetic event MTC tumorigenesis. Thus, the aim study was to evaluate frequency Polish population and relate obtained data presence mutations. Material methods: (RET, genes) were evaluated 78 snap-frozen samples (57 21 hereditary) by direct sequencing. Next,...
We studied the associations between 3'UTR genetic variants in ADME genes, clinical factors, and risk of breast cancer chemotherapy toxicity. Those factors were tested relation to seven symptoms belonging myelotoxicity (anemia, leukopenia, neutropenia), gastrointestinal side effects (vomiting, nausea), nephrotoxicity, hepatotoxicity, occurring overall, early, or recurrent settings. The cumulative overall anemia was connected with
Transcriptome of papillary thyroid cancer (PTC) is well characterized and correlates with some prognostic genotypic factors, but data addressing the interaction between PTC tumor microenvironment (TME) are scarce. Therefore, in present study, we aimed to assess impact TME on gene expression profile PTC. We evaluated normal cells isolated by laser capture microdissection whole tissue slides corresponding entire tumor. included 26 microdissected samples for analysis (HG-U133 PLUS 2.0,...
Molecular mechanisms of distant metastases (M1) in papillary thyroid cancer (PTC) are poorly understood. We attempted to analyze the gene expression profile PTC primary tumors seek genes associated with M1 status and characterize their molecular function. One hundred twenty-three patients, including 36 cases, were subjected transcriptome oligonucleotide microarray analyses: (set A-U133, set B-HG 1.0 ST) at transcript group level (limma, enrichment analysis (GSEA)). An additional independent...
Mechanisms driving the invasiveness of follicular thyroid cancer (FTC) are not fully understood. In our study, we undertook an unsupervised analysis set tumours (adenomas (FTA) and carcinomas) to verify whether malignant phenotype influences major sources variability in dataset.The core samples consisted 52 (27 FTC, 25 FTA). Total RNA was analysed by oligonucleotide microarray (HG-U133 Plus 2.0). Principal Component Analysis (PCA) applied as a main method analysis.An biological character...
A crucial issue in the management of thyroid nodules is to estimate, as accurately possible, malignancy risk lesions. The key tool for stratification fine needle aspiration biopsy. Unfortunately, approximately 20 % biopsy results are indeterminate. assigned these categories does not allow unequivocal further management.We aimed assess indeterminate Polish population, and analyze effectiveness clinical decisions after an cytological diagnosis practice.The retrospective analysis included 222...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)
The unique oncogenic duo of BRAF and TERT promoter ( p) variants was demonstrated to be associated with aggressiveness poor prognosis in several different cancer types, including melanoma thyroid cancer. It has been shown that the coexistence p a significantly more substantial impact on clinical outcomes than presence mutated or alone. At same time, co-occurrence may also Achilles Heel cells context targeted therapies' effectiveness. This paper aims summarize data from tumors which...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)