A5048269774- Digital Holography and Microscopy
- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Advanced Fluorescence Microscopy Techniques
- Pancreatitis Pathology and Treatment
- Optical Coherence Tomography Applications
- Pancreatic function and diabetes
- Nanoparticles: synthesis and applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Optical Imaging Technologies
- Optical measurement and interference techniques
- Microplastics and Plastic Pollution
- Pancreatic and Hepatic Oncology Research
- Advanced X-ray Imaging Techniques
- Graphene and Nanomaterials Applications
- Spectroscopy and Chemometric Analyses
- Cellular Mechanics and Interactions
- Nanofabrication and Lithography Techniques
- Near-Field Optical Microscopy
- Nanoparticle-Based Drug Delivery
- Protein Tyrosine Phosphatases
- Nitric Oxide and Endothelin Effects
- Gut microbiota and health
- Advanced Electron Microscopy Techniques and Applications
University of Münster
2015-2024
Technologiezentrum Dresden (Germany)
2024
University Hospital Münster
2004-2019
Max Delbrück Center
2019
Institut für Hämatopathologie Hamburg
2019
GTx (United States)
2016
BASF (Germany)
2010
Institute for Bioengineering of Catalonia
2008
University of Twente
2008
Klinik und Poliklinik für Psychosomatik und Psychotherapie
2007
Digital holographic microscopy provides new facilities for contactless and marker-free quantitative phase contrast imaging. In this work, a digital method the integral refractive index determination of living single cells in cell culture medium is presented. Further, obtained information applied to full field thickness shape adherent pancreas tumor cells, as well analysis drug-induced dynamic changes cell's cytoskeleton. The results demonstrate that technique under conventional laboratory conditions.
Nanomaterials (NMs) display many unique and useful physico-chemical properties. However, reliable approaches are needed for risk assessment of NMs. The present study was performed in the FP7-MARINA project, with objective to identify evaluate vitro test methods toxicity order facilitate development an intelligent testing strategy (ITS). Six representative oxide NMs provided by EC-JRC Repository were tested nine laboratories. evaluated 12 cellular models representing 6 different target...
Abstract Background Engineered nanomaterials display unique properties that may have impact on human health, and thus require a reliable evaluation of their potential toxicity. Here, we performed standardized in vitro screening 23 engineered nanomaterials. We thoroughly characterized the physicochemical adapted three classical toxicity assays to eliminate nanomaterial interference. Nanomaterial was assessed ten representative cell lines. Results Six induced oxidative stress while only single...
Many interferometry-based quantitative phase contrast imaging techniques require a separately generated coherent reference wave. This results in low stability and the demand for precise adjustment of intensity ratio between object To overcome these problems, performance Michelson interferometer approach digital holographic microscopy was analyzed that avoids wave by superposition different image areas. It is shown this simplified arrangement yields improved stability. Furthermore, from...
Industrial nanoparticles are not developed to be compatible with in vitro cell culture assays which carried out isotonic solutions at physiological pH and often the presence of proteins. The tendency deagglomerate or agglomerate is strongly sensitive these parameters. state agglomeration protein corona bear an important influence on level toxic effects via change transport mechanisms surface coating. Here we rigorously characterized interaction media for nanotoxicology experiments. Beyond...
The potential of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for quantification cell culture quality was explored. Label-free QPI detached single cells in suspension performed by Michelson interferometer-based self-interference DHM. Two pancreatic tumor lines were chosen as cellular model and analyzed refractive index, volume, dry mass under varying conditions. Firstly, adequate numbers reliable statistics identified. Then, to characterize the performance...
Abstract We present the first demonstration of shot-noise limited supercontinuum-based spectral domain optical coherence tomography (SD-OCT) with an axial resolution 5.9 μm at a center wavelength 1370 nm. Current SD-OCT systems cannot be operated in detection regime because severe pulse-to-pulse relative intensity noise supercontinuum source. To overcome this disadvantage, we have developed low-noise source based on all-normal dispersion (ANDi) fiber, pumped by femtosecond laser. The...
The concept of endothelium-derived relaxing factor (EDRF) put forward in 1980 by Furchgott and Zawadzki implies that nitric oxide (NO) produced NO synthase (NOS) the endothelium diffuses to underlying vascular smooth muscle, where it modulates tone as well muscle cell (VSMC) proliferation increasing cGMP formation with subsequent activation cGMP-dependent protein kinase. According this concept, VSMC do not express NOS themselves. This attractive, simple scheme is now under considerable...
A method for the determination of integral refractive index living cells in suspension by digital holographic microscopy is described. Digital phase contrast images spherical are recorded, and radius as well determined fitting relation between cell thickness distribution to measured data. The algorithm only requires information about medium image scale microscope system. specific advantage subsequent focus correction allows a simultaneous investigation different planes. Results obtained from...
Hereditary pancreatitis has been found to be associated with germline mutations in the cationic trypsinogen (PRSS1) gene. Here we report a family hereditary that carries novel PRSS1 mutation (R122C). This cannot diagnosed conventional screening method using <i>Afl</i>III restriction enzyme digest. We therefore propose new assay based on digest <i>Bst</i>UI, technique permits detection of R122C addition most common R122H mutation, and even presence recently reported neutral polymorphism...
Cytotoxicity quantification of nanoparticles is commonly performed by biochemical assays to evaluate their biocompatibility and safety. We explored quantitative phase imaging (QPI) with digital holographic microscopy (DHM) as a time-resolved in vitro assay quantify effects caused three different types organic development for medical use. Label-free proliferation native cell populations facilitates cytotoxicity testing biomedical nanotechnology. Therefore, DHM images from measurements on...
Intracellular Ca<sup>2+</sup>-changes not only participate in important signaling pathways but have also been implicated a number of disease states including acute pancreatitis. To investigate the underlying mechanisms an experimental model mimicking human gallstone-induced pancreatitis, we ligated pancreatic duct Sprague-Dawley rats and NMRI mice for up to 6 h studied intrapancreatic changes dynamics [Ca<sup>2+</sup>]<sub>i</sub> isolated acini. In contrast bile ligation, obstruction...
Lung lining fluid is the first biological barrier nanoparticles (NPs) encounter during inhalation. As previous inhalation studies revealed considerable differences between surface functionalized NPs with respect to deposition and toxicity, our aim was investigate influence of lipid and/or protein binding on these processes. Thus, we analyzed a set including different SiO2 ZrO2 in pure phospholipids, CuroSurfTM purified native porcine pulmonary surfactant (nS). Lipid surprisingly low for...
Oxidative stress, a commonly used paradigm to explain nanoparticle (NP)-induced toxicity, results from an imbalance between reactive oxygen species (ROS) generation and detoxification. As one consequence, protein carbonyl levels may become enhanced. Thus, the qualitative quantitative description of carbonylation be characterize how biological systems respond oxidative stress induced by NPs. We investigated representative panel 24 NPs including functionalized amorphous silica (6), zirconium...
A modular digital holographic microscopy system for integration in commercial microscopes has been developed. The reconstruction of the digitally captured holograms is performed by application a spatial phase shifting non diffractive method. As consequence applied algorithms reconstructed images contain not disturbing terms "twin image" and "zero order". In combination with microscope lenses system's lateral resolution improved up to diffraction limit. Digital focus adjustment constant...
Abstract Leukocyte infiltration is an early and critical event in the development of acute pancreatitis. However, mechanism leukocyte transmigration into pancreas function leukocytes initiating pancreatitis are still poorly understood. Here, we studied role S100A9 (MRP14), a calcium binding protein specifically released by polymorph nuclear (PMN), course experimental Acute was induced repeated supramaximal caerulein injections deficient or wild‐type mice. We then determined expression,...
Abstract Background The oral uptake of nanoparticles is an important route human exposure and requires solid models for hazard assessment. While the systemic availability generally low, ingestion may not only affect gastrointestinal tissues but also intestinal microbes. gut microbiota contributes essentially to health, whereas microbial dysbiosis known promote several extra-intestinal diseases. Gut microbiota-derived metabolites, which are found in blood stream, serve as key molecular...
Insulin signal transduction involves the multisite docking protein insulin receptor substrate-1 (IRS-1) and a number of Src homology-2 (SH2) domain factors, including p85/p110 phosphatidylinositol 3-kinase, p110 GTPase-activating protein, phosphotyrosine-specific phosphatase PTP1D. In transfected baby hamster kidney cells, Rat1 fibroblasts, normal IM9 lymphoblasts, PTP1D directly binds activated receptor. This interaction is mediated by catalytic domain-proximal SH2 determinants...