A5026301467- Metal and Thin Film Mechanics
- Nanoparticles: synthesis and applications
- Nanofabrication and Lithography Techniques
- Diamond and Carbon-based Materials Research
- Ion-surface interactions and analysis
- Polymer Surface Interaction Studies
- Surface Modification and Superhydrophobicity
- Plasma Applications and Diagnostics
- Plasma Diagnostics and Applications
- Electrohydrodynamics and Fluid Dynamics
- 3D Printing in Biomedical Research
- Force Microscopy Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Molecular Junctions and Nanostructures
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Analytical Chemistry and Sensors
- Monoclonal and Polyclonal Antibodies Research
- Adhesion, Friction, and Surface Interactions
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Semiconductor materials and devices
- Polymer Nanocomposite Synthesis and Irradiation
- Mass Spectrometry Techniques and Applications
Joint Research Centre
1992-2022
Joint Research Centre
2010-2020
Joint Research Centre
2018
European Commission
2006-2017
Directorate-General Joint Research Centre
2017
Consumers Union
2017
Art Research Centre of the Slovak Academy of Sciences
2015
Istituto Superiore di Sanità
2012
Charles University
2011
Cyclotron (Netherlands)
2011
Nanomaterials (NMs) display many unique and useful physico-chemical properties. However, reliable approaches are needed for risk assessment of NMs. The present study was performed in the FP7-MARINA project, with objective to identify evaluate vitro test methods toxicity order facilitate development an intelligent testing strategy (ITS). Six representative oxide NMs provided by EC-JRC Repository were tested nine laboratories. evaluated 12 cellular models representing 6 different target...
We demonstrate that it is possible to identify the protein−nanoparticle interaction site at amino acid scale in solution. Using NMR, chemical shift perturbation analysis, and dynamic light scattering we have identified a specific domain of human ubiquitin interacts with gold nanoparticles. This method allows detailed structural analysis proteins absorbed onto surfaces nanoparticles physiological conditions will provide much needed experimental data for better modeling prediction interactions.
Interleukin 1 beta (IL-1β)-dependent inflammatory disorders, such as rheumatoid arthritis and psoriasis, pose a serious medical burden worldwide, where patients face lifetime of illness treatment. Organogold compounds have been used since the 1930s to treat rheumatic other IL-1β-dependent diseases and, though their mechanisms action are still unclear, there is evidence that gold interferes with transmission signalling. Here we show for first time citrate-stabilized nanoparticles, in size...
With the increasing use of nanomaterials, need for methods and assays to examine their immunosafety is becoming urgent, in particular nanomaterials that are deliberately administered human subjects (as case nanomedicines). To obtain reliable results, standardised vitro immunotoxicological tests should be used determine effects engineered nanoparticles on immune responses. However, before can standardised, it important suitable established validated. In a collaborative work between European...
Nanotechnology is an emerging field that involves the development, manufacture and measurement of materials systems in submicron to nanometer range. Its development expected have a large socio-economical impact practically all fields industrial activity. However, there still lack information about potential risks manufactured nanoparticles for environment human health. In this work, we studied cytotoxicity, genotoxicity morphological transforming activity cobalt (Co-nano) ions (Co(2+))...
Abstract The inactivation of bacteria and biomolecules using plasma discharges were investigated within the European project BIODECON. goal was to identify isolate mechanisms by combining dedicated beam experiments with especially designed reactors. reactors are based on a fully computer‐controlled, low‐pressure inductively‐coupled (ICP). Four these built distributed among consortium, thereby ensuring comparability results between teams. Based this combined effort, role UV light, chemical...
Abstract Background Cobalt-ferrite nanoparticles (Co-Fe NPs) are attractive for nanotechnology-based therapies. Thus, exploring their effect on viability of seven different cell lines representing organs the human body is highly important. Methods The toxicological effects Co-Fe NPs were studied by in-vitro exposure A549 and NCIH441 cell-lines (lung), precision-cut lung slices from rat, HepG2 cell-line (liver), MDCK (kidney), Caco-2 TC7 (intestine), TK6 (lymphoblasts) primary mouse...
We measure the structural and stability changes of proteins at nanomolar concentration upon interaction with nanoparticles. Using synchrotron radiation circular dichroism (SRCD), we a decrease 6 °C in thermal unfolding human serum albumin silver nanoparticles while this does not happen gold. The use SRCD allows measuring critical parameters on protein-nanoparticle interactions, it will provide experimental data relative key biological for nanotoxicology.
Despite human gastrointestinal exposure to nanoparticles (NPs), data on NPs toxicity in intestinal cells are quite scanty. In this study we evaluated the induced by zinc oxide (ZnO) and titanium dioxide (TiO2) Caco-2 cells. Only ZnO produced significant cytotoxicity, two different assays. The presence of foetal calf serum culture medium significantly reduced as well ion leakage NP-cell interaction. increased intracellular amount reactive oxygen species (ROS) after 6 h treatment. However,...
In this work, Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS), Principal Component Analysis (PCA) and X-ray Photoelectron Spectroscopy (XPS) have been used to characterize the surface chemistry gold substrates before after functionalization with thiol-modified glucose self-assembled monolayers subsequent biochemical specific recognition maltose binding protein (MBP). The results indicate that is achieved both on flat nanoparticles thus showing potential developed system as...
The wide use of titanium dioxide nanoparticles (TiO2 NPs) in industrial applications requires the investigation their effects on human health. In this context, we investigated nanosized and bulk titania two different crystalline forms (anatase rutile) vitro. By colony forming efficiency assay, a dose-dependent reduction clonogenic activity Balb/3T3 mouse fibroblasts was detected presence rutile, but not case anatase NPs. Similarly, cell transformation assay micronucleus test showed that...
Abstract Summary: Non‐equilibrium discharges in molecular gases and their mixtures have been extensively studied due to applicability different areas of surface treatment, cleaning functionalization. In this paper, the feasibility use low pressure for sterilization decontamination, i.e., a topic that has attracted great attention recently, is demonstrated discussed using examples bacterial spore depyrogenation endotoxins, as well elimination protein films. Image spores used sterilization,...
The toxicological effects of cobalt nanoparticles (Co-NPs) aggregates were examined and compared with those ions (Co-ions) using six different cell lines representing lung, liver, kidney, intestine, the immune system. Dose-response curves studied in concentration range 0.05–1.0mM, employing 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide test, neutral red, Alamar blue as end point assays following exposures for 48 72 h. Data analysis predictive modeling obtained data sets...
The mechanisms of sterilization and decontamination surfaces are compared in direct post discharge plasma treatments two low-pressure reactors, microwave inductively coupled plasma. It is shown that the removal various biomolecules, such as proteins, pyrogens or peptides, can be obtained at high rates low temperatures (ICP) by using Ar/O2 mixtures. Similar efficiency for bacterial spores. Analysis conditions illustrates role ion bombardment associated with O radicals, leading to a fast...
The aim of the present study was to investigate effect silver nanoparticles (AgNP) different sizes toward two primary producer aquatic species. Thalassiosira pseudonana and Synechococcus sp. have been selected as representative models for lower trophic organisms in marine freshwater habitats, respectively. Time-dependent cellular growth measured upon exposure both AgNP nitrate (AgNO(3)). In addition, behavior waters has followed by CPS disc centrifuge, time frame studies, kinetic release...