A5101599181- Metastasis and carcinoma case studies
- Mesenchymal stem cell research
- Gastric Cancer Management and Outcomes
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Gastrointestinal Tumor Research and Treatment
- Immune cells in cancer
- Multiple and Secondary Primary Cancers
- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Cardiomyopathy and Myosin Studies
- Bone health and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Hepatocellular Carcinoma Treatment and Prognosis
- Pluripotent Stem Cells Research
- Cancer Diagnosis and Treatment
- Colorectal Cancer Surgical Treatments
- Breast Cancer Treatment Studies
- Intraperitoneal and Appendiceal Malignancies
- Zebrafish Biomedical Research Applications
- Breast Lesions and Carcinomas
- Drug-Induced Adverse Reactions
- Muscle activation and electromyography studies
- Renal cell carcinoma treatment
- Prosthetics and Rehabilitation Robotics
University of Tsukuba
2010-2025
Life & Brain (Germany)
2018-2021
University of Bonn
2018-2021
University Hospital Bonn
2021
Hyogo Medical University
2021
Government of Japan
2016
Ministry of Health Labour and Welfare
2015-2016
Aichi Cancer Center
2016
Tokyo Metropolitan University
2013-2015
Kansai Rosai Hospital
2014-2015
Mesenchymal stem cells (MSCs) possess self-renewal and multipotential differentiation abilities, they are thought to be one of the most reliable cell sources for a variety therapies. Recently, therapy using MSCs has been studied as novel therapeutic approach cancers that show refractory progress poor prognosis. from different tissues have properties. However, effect MSC properties on their application in anticancer therapies not thoroughly investigated. In this study, characterize sources,...
New strategies in the field of cardiac regeneration are directed at identifying proliferation-inducing substances to induce regrowth myocardium. Current screening assays utilize neonatal cardiomyocytes and markers for cytokinesis, such as Aurora B-kinase. However, detection cardiomyocyte division is complicated because cell cycle variants, particular, binucleation.To analyze process binucleation identify definitive discriminators variants authentic division.Herein, we demonstrate by direct...
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM cleared specimens), low-cost ($20,000-50,000), low-expertise (one-day installation by non-expert), yet practical do-it-yourself microscope as solution this bottleneck. Even the most fundamental configuration enables multi-color imaging whole mouse brains and...
Supine plasma concentration of norepinephrine (PNE), epinephrine (PE), and aldosterone (PA), renin activity (PRA), blood volume (BV) were measured in 25 normotensive 11 hypertensive patients with biopsy-proven glomerulonephritis who had serum creatinine concentrations less than 1.6 mg/dl, 20 control subjects. PNE PE according to the trihydroxyindol method using high pressure liquid chromatography. Renal clearances p-aminohippurate (CPAH) endogenous (Ccr) also determined. Age, BV, 24-hour...
Human placenta is an attractive source of mesenchymal stem cells (MSC) for regenerative medicine. The cell surface markers expressed on MSC have been proposed as useful tools the isolation from other populations. However, correlation between expression and ability to support tissue regeneration in vivo has not well examined. Here, we established several lines human examined their differentiate toward lineages. We found that CD349/frizzled-9, a receptor Wnt ligands, was positive...
Mesenchymal stem cells (MSCs) are highly useful in a variety of cell therapies owing to their multipotential differentiation capability. MSCs derived from umbilical cord blood generally isolated by plastic adherence without using specific surface markers and examined for osteogenic, adipogenic, chondrogenic properties retrospectively. Here, we report 2 subpopulations MSCs, separated based on aldehyde dehydrogenase (ALDH) activity. with high ALDH activity (Alde-High) proliferated more than...
Endothelial progenitor cells (EPCs) have the ability to form new blood vessels and protect ischemic tissues from damage. We previously reported that EPCs with low activity of aldehyde dehydrogenase (Alde-Low EPCs) possess greater treat compared Alde-High EPCs. The expression level hypoxia-inducible factors (HIFs), HIF-1α HIF-2α, was found be in Alde-Low than However, precise role HIF regulation EPC remains obscure. In this study, we demonstrate a critical HIF-2α its target gene CXCR4 for...
The first issue of Nature Medicine published 20 years ago featured an article that reported Japan's critical situation regarding clinical trials, calling for major reform. Twenty later, Japan has enacted three laws to promote the use regenerative medicine as a national policy. law be was Regenerative Promotion Act, which represents country's determination work toward promotion medicine. Subsequently, Pharmaceuticals, Medical Devices, and Other Therapeutic Products Act (PMD Act) on Safety (RM...
The prevalence of type 2 diabetes mellitus (T2DM), which leads to diabetic complications, has been increasing worldwide. possible applications T2DM-derived stem cells in cell therapy are limited because their characteristics still not fully understood. In this study, we characterized adipose tissue-derived mesenchymal (AT-MSCs) from patients (dAT-MSCs) and found that insulin receptor substrate-1 (IRS-1) was highly phosphorylated at serine 636/639 dAT-MSCs. Moreover, early growth response...
Abstract Ventricular tachycardia (VT) is the most common and potentially lethal complication following myocardial infarction (MI). Biological correction of conduction inhomogeneity that underlies re-entry could be a major advance in therapy. As minimal increases infarcted tissue markedly influence VT susceptibility, we reasoned enhanced propagation electrical signal between non-excitable cells within resolving infarct might comprise simple means to decrease post-infarction arrhythmia risk....
Abstract An amino acid exchange (P209L) in the HSPB8 binding site of human co-chaperone BAG3 gives rise to severe childhood cardiomyopathy. To phenocopy disease mice and gain insight into its mechanisms, we generated humanized transgenic mouse models. Expression P209L -eGFP caused Z-disc disintegration formation protein aggregates. This was accompanied by massive fibrosis resulting early-onset restrictive cardiomyopathy with increased mortality as observed patients. RNA-Seq proteomics...
Mesenchymal stem cells (MSC) are multipotent and possess high proliferative activity, thus thought to be a reliable cell source for therapies. Here, we isolated MSC from adult tissues--bone marrow (BM-MSC), dental tissue (DT-MSC), adipose (AT-MSC)--to compare how autotransplantation of these effectively supports the repair bone fracture ischemic tissue. An analysis by in vitro differentiation assays showed no significant difference among MSC. The degree calcification at joint region was...
BACKGROUND: Aortic dissection (AD) is the separation of medial layers aorta and a major cause death in patients with connective tissue disorders such as Marfan syndrome. However, molecular triggers instigating AD, its temporospatial progression, how vascular cells each vessel layer interact participate pathological process remain incompletely understood. To unravel underlying mechanisms we generated spontaneous AD mouse model. METHODS: We incorporated novel missense variant (p.G234D) FBN1 ,...
Retinitis pigmentosa is a group of inherited eye disorders that result in profound vision loss with characteristic retinal neuronal degeneration and vasculature attenuation. In mouse model retinitis pigmentosa, endothelial progenitor cells (EPC) from bone marrow rescued the photoreceptors. However, mechanisms cell types underlying these protective effects were uncertain. We divided EPC, which contribute to angiogenesis, into two subpopulations based on their aldehyde dehydrogenase (ALDH)...
The variants of the hypoxia-inducible factor-3α gene HIF-3α and NEPAS are known to repress transcriptional activities driven by HIF-1α HIF-2α. Although has been shown play an important role in vascular remodeling during lung development, little is about roles adult function. Here, we examined pulmonary endothelial cells (ECs) isolated from wild-type (WT) functional knockout (KO) mice. expression levels angiogenic factors (Flk1, Ang2 Tie2) were significantly greater KO ECs than those WT...
Glucocorticoids are steroid hormones used as anti-inflammatory treatments. However, this strong immunomodulation causes undesirable side effects that impair bones, such osteoporosis. Glucocorticoid therapy is a major risk factor for developing steroid-induced osteonecrosis of the femur head (ONFH). Since ONFH incurable, with mesenchymal stem cells (MSCs) can differentiate into osteoblasts first-line choice. Bone marrow-derived MSCs (BM-MSCs) often source cell ONFH, but their proliferative...
Mesenchymal stem cells (MSCs) are multipotent and considered to be of great potential for regenerative medicine. We could show recently (Breitbach, Kimura et al. 2018) that a subpopulation MSCs as well sinusoidal endothelial (sECs) in the bone marrow (BM) CD73-EGFP reporter mice labeled vivo. took advantage this model explore plasticity osteogenic CD73-EGFP+ vitro their role response upon lesion Herein we isolated displayed more pronounced stemness stronger differentiation capacity into...
Erythropoiesis is strongly influenced by the interactions between stromal cells and erythroid progenitors, as well a key regulatory factor, erythropoietin (EPO). We previously generated mice with knockdown mutation of Hif-2α (referred to kd/kd) found that these kd/kd exhibited normocytic anemia, even though EPO expression was not severely affected. However, VCAM-1 in spleen endothelial (EC), which regulated HIF-2α, impaired, resulting defective maturation. A deficiency HIF-2α clearly led...
Embryonic stem (ES) cells are useful for elucidating the molecular mechanisms of cell fate decision in early development mammals. It has been shown that aggregate culture ES efficiently induces neuroectoderm differentiation. However, mechanism leads to selective neural differentiation is not fully understood. Here, we demonstrate oxygen-sensitive hypoxia-inducible transcription factor, Hif-1α, an essential regulator commitment cells. We found a hypoxic environment spontaneously established...
Both bevacizumab and anti-epithelial growth factor receptor (EGFR) agents (e.g. cetuximab panitumumab) are sequentially used for metastatic colorectal cancer (mCRC). Their co-administration as a first-line treatment does not improve outcome, indicating that there negative interactions between these agents. A long-term pharmacokinetics study demonstrated serum persistence of following termination 6 months after the last administration. This prompted us to investigate impact short intervals...
Abstract The maladaptive remodeling of vessel walls with neointima formation is a common feature proliferative vascular diseases. It has been proposed that caused by the dedifferentiation mature smooth muscle cells (SMCs). Recent evidence suggests adventitial also participate in formation; however, their cellular dynamics are not fully understood. In this study, we utilized lineage tracing model platelet-derived growth factor receptor alpha (PDGFRa) and examined behavior during homeostasis...