A5056908833- DNA and Nucleic Acid Chemistry
- Protein Structure and Dynamics
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Ubiquitin and proteasome pathways
- Robotics and Automated Systems
- Computational Drug Discovery Methods
- Advanced Image Processing Techniques
- Mitochondrial Function and Pathology
- Hemodynamic Monitoring and Therapy
- Adipose Tissue and Metabolism
- Microbial Natural Products and Biosynthesis
- Microtubule and mitosis dynamics
- Lysosomal Storage Disorders Research
- Cancer-related Molecular Pathways
- Chemical Synthesis and Analysis
- Photoreceptor and optogenetics research
- Advanced biosensing and bioanalysis techniques
- ATP Synthase and ATPases Research
- Nicotinic Acetylcholine Receptors Study
- Animal Behavior and Welfare Studies
- Enzyme Structure and Function
- Image and Signal Denoising Methods
- Image Enhancement Techniques
- Fungal and yeast genetics research
Kitasato University
2017-2024
Gifu University
2021-2024
Gifu University Hospital
2023
Hiroshima City University
2020-2021
Kyoto University
1991-2021
Hiroshima University
2001-2020
ETH Zurich
2019
York University
2019
Tokyo University of Science
2018
The University of Kitakyushu
2017
This paper reviews the NTIRE 2019 challenge on real image denoising with focus proposed methods and their results. The has two tracks for quantitatively evaluating performance in (1) Bayer-pattern raw-RGB (2) standard RGB (sRGB) color spaces. had 216 220 registered participants, respectively. A total of 15 teams, proposing 17 methods, competed final phase challenge. by teams represent current state-of-the-art targeting noisy images.
Abstract ATP levels may represent fundamental health conditions of cells. However, precise measurement intracellular in living cells is hindered by the lack suitable methodologies. Here, we developed a novel biosensor termed “BTeam”. BTeam comprises yellow fluorescent protein (YFP), binding domain ε subunit bacterial synthase, and an ATP-nonconsuming luciferase (NLuc). To attain emission, simply required NLuc substrate. showed elevated bioluminescence resonance energy transfer efficiency...
This paper reviews the first NTIRE challenge on perceptual image enhancement with focus proposed solutions and results. The participating teams were solving a real-world photo problem, where goal was to map low-quality photos from iPhone 3GS device same captured Canon 70D DSLR camera. considered problem embraced number of computer vision subtasks, such as denoising, resolution sharpness enhancement, color/contrast/exposure adjustment, etc. target metric used in this combined PSNR SSIM scores...
We isolated a fission yeast putative protein serine/threonine phosphatase gene designated ppe1+ by hybridization. The predicted amino acid sequence is similar to those of the ppa2 (53% identity) and dis2 (39%) phosphatases, highly budding SIT4 (72%), Drosophila PPV (68%) rabbit PPX (61%) phosphatases. Antibodies against ppe1 identified 37-kd polypeptide in yeast. A disruption (designated delta ppe1) caused cold-sensitive lethality short, pear-shaped cells. These phenotypes were fully...
Branched-chain amino acids (BCAAs) are essential acids, controlling cellular metabolic processes as signaling molecules; therefore, utilization of intracellular BCAAs may be regulated by the availability nutrients in environment. However, spatial and temporal regulation BCAA concentration response to environmental conditions has been unclear due lack suitable methods for measuring concentrations inside single living cells. Here, we developed a Förster resonance energy transfer (FRET)-based...
Abstract The reverse transsulfuration pathway, which is composed of cystathionine β-synthase (CBS) and γ-lyase (CGL), plays a role to synthesize l -cysteine using -serine the sulfur atom in -methionine. A plant-derived lactic acid bacterium Lactobacillus plantarum SN35N has been previously found harbor gene cluster encoding CBS- CGL-like enzymes. In addition, it demonstrated that L. CBS can from O -acetyl- -homocysteine. aim this study characterize enzymatic functions CGL. We have enzyme...
Abstract We evaluated the docking accuracy of ArgusLab, which is a freely available software program for computational docking, using experimentally determined protein–ligand complex structures. Investigations roles parameters used calculations in ArgusLab were carried out addition to evaluation software. The results indicate that one engine GADock, superior terms accuracy, and another engine, ArgusDock, advantageous time it provides. In pose construction step, larger population sizes...
Abstract Cystathionine β‐synthase (CBS) catalyzes the formation of l ‐cystathionine from ‐serine and ‐homocysteine. The resulting is decomposed into ‐cysteine, ammonia, α‐ketobutylic acid by cystathionine γ‐lyase (CGL). This reverse transsulfuration pathway, which catalyzed both enzymes, mainly occurs in eukaryotic cells. CBS CGL have recently been recognized as major physiological enzymes for generation hydrogen sulfide (H 2 S). In some bacteria, including plant‐derived lactic bacterium...
Abstract During periods of crisis, cells must compensate to survive. To this end, may need alter the subcellular localization crucial proteins. Here, we show that during starvation, VCP, most abundant soluble ATPase, relocalizes and forms aggregate‐like structures at perinuclear regions in PC3 prostate cancer cells. This movement is associated with a lowered metabolic state, which mitochondrial activity ROS production are reduced. VCP appears explicitly sense glutamine levels, as removal...
Anthracycline, a widely used anti-cancer agent, can elicit irreversible cardiomyopathy called anthracycline-induced (ACM). In this report, we describe 5 cases of severe chronic heart failure due to ACM effectively treated with the beta-blocker, carvedilol. Their left ventricular function as well cardiac symptoms were persistently improved after treatment carvedilol, suggesting that carvedilol may be an effective therapeutic strategy for demonstrated in other forms failure.
Abstract In Chinese medicine, herbal medicine is commonly used to treat individuals suffering from many types of diseases. We thus expected that some medicines would contain promising compounds for cancer chemotherapy. Indeed, we found Sanguisorba officinalis extracts strongly inhibit the growth B16F10 melanoma cells, and identified ellagic acid (EA) as responsible ingredient. cells treated with EA exhibited strong G1 arrest accompanied by accumulation p53, followed inactivation AKT....
In recent years, the function of pharmacological chaperones as a "thermodynamic stabilizer" has been attracting attention in combination therapy. The coadministration chaperone and recombinant human acid α-glucosidase (rhGAA) leads to improved stability maturation by binding folded state rhGAA thereby promotes enzyme delivery. This study provides first example strategy design high-affinity ligand toward lysosomal (GAA) focusing on alkyl branches 1-deoxynojirimycin (DNJ); 5-C-heptyl-DNJ...
We report extensive conformational searches of the gas-phase neutral nicotine, nornicotine, and their protonated analogs pathways barriers for interconversion between various isomers that are based on ab initio second-order Møller-Plesset perturbation (MP2) electronic structure calculations. Initial were performed with 6-31G(d,p), energetics most important structures further refined from geometry optimizations larger aug-cc-pVTZ basis set. On calculated free energies at T = 298 K molecules,...
We report a three-dimensional quantitative structure–activity relationship (3D-QSAR) analysis of CDK2 inhibitors using fragment molecular orbital (FMO) calculations and partial least squares (PLS) regression. In our analysis, binding energies individual amino acids energy single ligand in protein–ligand complex are evaluated by FMO used as descriptors PLS regression to estimate biological activities the ligands. The was applied system protein its effectiveness method tested. Application...
Glycocalyx is present on the surface of healthy endothelium, and concentration serum syndecan-1 can serve as an injury marker. This study aimed to assess endothelial using a marker glycocalyx in patients who underwent hemodialysis. In this single-center, retrospective, observational study, 145 hemodialysis at Gifu University Hospital between March 2017 December 2019 were enrolled. The median dialysis period time 63 months 3.7 h, respectively. significantly increased from 124.6 ± 107.8 ng/ml...
The electronic states of chemically doped DNA polynucleotides [poly(dG)-poly(dC)] have been studied by photoelectron spectroscopy, x-ray absorption and Raman in order to understand the charge conduction mechanism this material. Upon iodine doping (i.e., hole doping), we clearly observed radical cation formation on bases, appearance additional occupied unoccupied within band gap poly(dG)-poly(dC). Referring analogous studies for $\ensuremath{\pi}$-conjugated conductive polymers, point out...
Some point mutations in β-glucocerebrosidase cause either improper folding or instability of this protein, resulting Gaucher disease. Pharmacological chaperones bind to the mutant enzyme and stabilize enzyme; thus, pharmacological chaperone therapy was proposed as a potential treatment for The binding affinities α-1-C-alkyl 1,4-dideoxy-1,4-imino-d-arabinitol (DAB) derivatives, which act β-glucocerebrosidase, abruptly increased upon elongation their alkyl chain. In study, primary causes such...
Structural data of protein–DNA complex show redundancy and flexibility in base–amino acid interactions. To understand the origin specificity recognition, we calculated interaction free energy, enthalpy, entropy, minimum energy maps for AT-Asn, GC-Asn, AT-Ser, GC-Ser by means a set ab initio force field with extensive conformational sampling. We found that most preferable interactions these pairs are stabilized hydrogen bonding, mainly enthalpy driven. However, minima not necessarily same as...
As promising antifungal agents, the eight stereoisomers of eushearilide, including natural compound, were synthesized relying on an asymmetric Mukaiyama aldol reaction, Julia-Kocienski olefination, and Shiina macrolactonization. Moreover, their in vitro antimicrobial activities against some fungi bacteria evaluated by disk-diffusion method, which revealed that not only eushearilide but also its exhibited significant activity a variety bacteria.
This study aimed to determine the impact of augmented renal clearance (ARC) on anticoagulation therapy in critically ill patients with coronavirus disease 2019 (COVID-19).
A series of 11-[4-(cinnamyl)-1-piperazinyl]-6,11-dihydrodibenz[b,e] oxepins and related compounds were synthesized evaluated for their protective activities against complete ischemia, normobaric hypoxia, lipidperoxidation convulsion. Structure-activity relationship studies this led to the finding (E)-1-(3-fluoro-6,11-dihydrodibenz[b,e]oxepin-11-yl)-4-(3- phenyl-2-propenyl)piperazine dimaleate (50), AJ-3941 with most appropriate property combined pharmacological activities. Compound 50 also...
Fluctuations in food availability and shifts temperature are typical environmental changes experienced by animals. These sometimes portend more severe changes; e.g., chilly north winds precede the onset of winter. Such telltale signs may be indicators for animals to prepare such a shift. Here we show that HEK293A cells, cultured under starvation conditions, can "memorize" short exposure cold (15 °C), which was evidenced their higher survival rate compared cells continuously grown at 37 °C....