A5004591239- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Plant responses to water stress
- Plant Surface Properties and Treatments
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Rice Cultivation and Yield Improvement
- Plant Micronutrient Interactions and Effects
- Genetic Mapping and Diversity in Plants and Animals
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Insect symbiosis and bacterial influences
- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Food Supply Chain Traceability
- Research in Cotton Cultivation
- Biofuel production and bioconversion
- GABA and Rice Research
- Identification and Quantification in Food
South China Agricultural University
2009-2024
Key Laboratory of Guangdong Province
2016-2022
Purdue University West Lafayette
2021
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2012-2018
Planta
2009
Abstract Hybrid rice breeding offers an important strategy to improve production, in which the cultivation of a male sterile line is key success cross-breeding. CRISPR/Cas9 systems have been widely used target-site genome editing, whereas their application for crop genetic improvement has rarely reported. Here, using system, we induced specific mutations TMS5 , most applied thermo-sensitive genic sterility (TGMS) gene China, and developed new “transgene clean” TGMS lines. We designed 10...
Proteins of the ARGONAUTE (AGO) family function in epigenetic regulation gene expression. Although rice (Oryza sativa) genome encodes 19 predicted AGO proteins, few their functions have thus far been characterized. Here, we show that protein OsAGO2 regulates anther development rice. was highly expressed anthers. Knockdown led to overaccumulation reactive oxygen species (ROS) and abnormal development, causing premature initiation tapetal programmed cell death (PCD) pollen abortion. The...
Cuticular wax is a class of organic compounds that comprises the outermost layer plant surfaces. Plant cuticular wax, last barrier self-defense, plays an important role in growth and development. The OsGL1-6 gene, member fatty aldehyde decarbonylase gene family, highly homologous to Arabidopsis CER1, which involved biosynthesis. However, whether participates biosynthesis remains unknown. In this study, antisense-RNA vector driven by its own promoter was constructed introduced into rice...
The NAC (NAM, AFAT, and CUC) transcription factors play critical roles in rice (Oryza sativa) development stress regulation. Overexpressing a microRNA (miR164b)-resistant OsNAC2 mutant gene, which generates transcripts that cannot be targeted by miR164b, improves plant architecture yield; however, the performance of these mOsNAC2-overexpressing lines, named ZUOErN3 ZUOErN4, under abiotic conditions such as drought have not yet been fully characterized.In this study, we showed germination...
Plant architecture is a major target of rice (Oryza sativa) breeding and selection, but the underlying regulatory networks remain unclear. Here, we overexpressed an OsNAC2 mutant (OErN) that cannot be cleaved by miRNA miR164b. OErN plants had better plant longer panicles, produced more grains. The parental line averaged 12.2 primary 31.5 secondary branches in main panicles; two lines 15.0 15.2 primary, 41.5 44.3 branches. In large-scale field trials, at least 58.62% total grain (by weight)...
Cuticle waxes, which are primarily comprised of very-long-chain (VLC) alkanes, play an important role in plant reproductive development. ECERIFERUM1 (CER1) is recognized as the core element for VLC alkane biosynthesis Arabidopsis (Arabidopsis thaliana). However, genes involved rice remain unclear, and alkane-form pathway has still to be further explored. Here, we show that OsCER1, a homology CER1, functions alkanes biosynthesis, also could regulate anther development plastids differentiation...
Summary Most plant pentatricopeptide repeat (PPR) proteins localize to and function inside plastids mitochondria. However, the of PPRs that only cytoplasm remains unknown. Here, we demonstrated rice ( Oryza sativa ) PPR protein CYTOPLASM‐LOCALIZED PPR1 (OsCPPR1) contributes pollen development localizes cytoplasm. Knocking down OsCPPR1 led abnormal plastid in tapetal cells, prolonged programmed cell death (PCD) tapetum degradation, significantly reduced fertility. Transcriptome analysis...
The Papaya ringspot virus (PRSV)-resistant genetically modified (GM) papaya 'Huanong No.1' has been certified as safe for consumption and widely planted in China about 18 years. To protect consumers' rights facilitate government supervision monitoring, it is necessary to establish a simple, rapid, specific detection method No.1'. Herein, we developed platform based on recombinase polymerase amplification (RPA) coupled with CRISPR-Cas12a the of RPA-CRISPR-Cas12a was found have high...
Abstract Grain size is one of the crucial factors determining grain yield. However, genetic and molecular mechanisms florigen repression complexes (FRCs) underlying in rice (Oryza sativa L.) have not been reported. Here, we report that CENTRORADIALIS (CEN) family member OsCEN2 (also known as Rice TFL1/CEN homolog, RCN1), a phosphatidylethanolamine-binding protein (PEBP) protein, negatively controls rice. Overexpression led to small grains, knockout resulted large, heavy grains. influenced by...
Antisense and RNA interference (RNAi)-mediated gene silencing systems are powerful reverse genetic methods for studying function. Most RNAi antisense experiments used constitutive promoters to drive the expression of RNAi/antisense transgenes; however, several reports showed that were not expressed in all cell types cereal plants, suggesting promoter effective certain tissues/organs. To develop an alternative method complements systems, we constructed and/or transgenes four rice genes using...
Leaf senescence is a highly complex and meticulous regulatory process, the disruption of any factor involved in leaf might lead to premature or delayed thus result reduced increased crop yields. Despite sincere efforts by scientists, there remain many unsolved problems related factors molecular mechanisms senescence.This study successfully revealed that OsHXK1 was expressed senescent leaves rice. The upregulation led rice leaves, decreased level chlorophyll, damage chloroplast structure....
ARGONAUTE 1 (AGO1) proteins can recruit small RNAs to regulate gene expression, involving several growth and development processes in Arabidopsis. Rice genome contains four AGO1 genes, OsAGO1a OsAGO1d. However, the regulatory functions rice of each are still unknown.We obtained overexpression RNAi transgenic lines OsAGO1 gene. only up- down-regulation OsAGO1b caused multiple abnormal phenotypic changes rice, indicating that is key player organ compared with other three OsAGO1s. qRT-PCR...