A5077988602- Gut microbiota and health
- Diet and metabolism studies
- Clostridium difficile and Clostridium perfringens research
- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Dysphagia Assessment and Management
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- Diet, Metabolism, and Disease
- Metabolomics and Mass Spectrometry Studies
Institute of Microbiology
2019-2021
Chinese Academy of Sciences
2019-2021
Academy of Military Medical Sciences
2016-2017
Abstract Inulin has been used as a prebiotic to alleviate glucose and lipid metabolism disorders in mice humans by modulating the gut microbiota. However, mechanism underlying alleviation of metabolic inulin through interactions between microbiota host cells is unclear. We use ob/ob model study effect on cecal 16S rRNA gene amplicon sequencing its interaction with transcriptomics. The inulin-supplemented diet improved disorder parameters mice, alleviating fat accumulation intolerance. α...
Functional oligosaccharides, known as prebiotics, and ordinary dietary fiber have important roles in modulating the structure of intestinal microbiota. To investigate their effects on microecosystem, three kinds diets containing different prebiotics were used to feed mice for 3 weeks, follows: GI (galacto-oligosaccharides inulin), PF (polydextrose insoluble from bran), a GI/PF mixture (GI PF, 1:1), 16S rRNA gene sequencing metabolic analysis feces then conducted. Compared control group, had...
Cross talk occurs between the human gut and lung through a gut-lung axis involving microbiota. However, signatures of microbiota after active Mycobacterium tuberculosis infection have not been fully understood. Here, we investigated changes in (TB) patients by shotgun sequencing microbiomes 31 healthy controls 46 patients. We observed dramatic as reflected significant decreases species number microbial diversity. The TB were mostly featured striking decrease short-chain fatty acids...
Intestinal microbiota operated as a whole and was closely related with human health. Previous studies had suggested close relationship between liver cirrhosis (LC) gut microbiota.To determine the functional characteristic of intestinal specific for cirrhosis, fecal metaproteome three LC patients Child-Turcotte-Pugh (CTP) score A, B, C, their spouse were first compared using high-throughput approach based on denaturing polyacrylamide gel electrophoresis liquid chromatography-tandem mass...
Patients with pulmonary tuberculosis (TB) undergoing anti-tuberculosis (anti-TB) treatment were previously reported to present gut bacterial microbiota dysbiosis, but the role of mycobiota has not been reported. Here, we conducted a follow-up study 29 naive TB patients who received first-line anti-TB drug treatment; collected their fecal samples at different time points, as well 22 from healthy subjects. Fungal ITS2 and 16S rRNA amplicon sequencing used analyze effects active on microbiota....
The gut flora are widely involved in the cometabolism with host and have evident effects on metabolic phenotype of host. This study performed a metabolome analysis intestinal microbiota specific for liver cirrhosis. population included patients Child-Turcotte-Pugh score A (AP, n = 5) B (BP, 5), control subjects (NM, 3). Metagenomic DNA from fecal was extracted followed by metagenomic sequencing through Illumina MiSeq high throughput 16S rRNA regions. detection metabolites samples using...
Bifidobacteria are frequently used in probiotic food and dairy products. Bifidobacterium bifidum S17 is a promising candidate strain that displays strong adhesion to intestinal epithelial cells elicits potent anti-inflammatory capacity both vitro murine models of colitis. The recently sequenced genome B. has size about 2.2 Mb encodes 1,782 predicted protein-coding genes. In the present study, comprehensive proteomic profiling was carried out identify characterize proteins expressed by S17. A...