A5004667716- Biofuel production and bioconversion
- Meat and Animal Product Quality
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Enzyme Production and Characterization
- Animal Nutrition and Physiology
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Biochemical effects in animals
- Anaerobic Digestion and Biogas Production
- Advanced Cellulose Research Studies
- Enzyme Catalysis and Immobilization
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Antibiotic Use and Resistance
- Alzheimer's disease research and treatments
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Peptides and Activities
- Steroid Chemistry and Biochemistry
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial metabolism and enzyme function
- Vibrio bacteria research studies
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
Salem VA Medical Center
2025
Vanderbilt University Medical Center
2023-2025
Dow University of Health Sciences
2025
All-Russian Scientific Research Institute of the Dairy Industry
2023-2024
COMSATS University Islamabad
2016-2024
Health Services Academy
2023-2024
The University of Texas Health Science Center at Houston
2017-2024
Vardhman Mahavir Medical College & Safdarjung Hospital
2024
Vanderbilt University
2023-2024
Clínica Alemana
2022-2023
Metals are found associated with β-pleated sheets of Aβ42 in vivo and may be involved their formation. Metal chelation has been proposed as a therapy for Alzheimer's disease on the basis that it safely dissolve precipitated Aβ peptides. We hav
Vascular dementia (VaD) is a major contributor to the syndrome and described as having problems with reasoning, planning, judgment, memory caused by impaired blood flow brain damage vessels resulting from events such stroke. There are variety of etiologies that contribute development vascular cognitive impairment VaD, these often associated other dementia-related pathologies Alzheimer disease. The diagnosis VaD difficult due number types lesions their locations in brain. Factors increase...
Bacteria have developed several evolutionary strategies to protect their cell membranes (CMs) from the attack of antibiotics and antimicrobial peptides (AMPs) produced by innate immune system, including remodeling phospholipid content localization. Multidrug-resistant Enterococcus faecalis, an opportunistic human pathogen, evolves resistance lipopeptide daptomycin AMPs diverting antibiotic away critical septal targets using CM anionic redistribution. The LiaFSR stress response system...
Tests were made to determine the effects of inorganic and organic sulfur sources on degradation cellulose methane in a chemically defined medium with sulfur-poor inoculum prepared from sewage sludge. The results show that source about 0.85 mM concentration is essential for CH4. However, production CH4 CO2 H2 provided headspace occurred 0.1 sulfate or sulfide. At 9 concentration, all compounds other than inhibited both formation, this inhibition increased order thiosulfate less sulfite...
A new genus of cellulolytic, gram-negative, nonsporeforming, anaerobic bacteria is described. The colonies produced by these on cellulose agar were round, clear, translucent, and cream-colored had an undulate margin. Single cells the bacterium straight to slightly curved rods 0.5 0.8 µm wide 4 10 long motile means a single flagellum located one-third distance from end cell. Among various substrates tested, only cellulose, cellobiose, salicin able support growth. major fermentation products...
SUMMARY To study the effects of glycolysis on tenderness poultry breast meat, premortem and postmortem in muscle tissue was controlled either by epinephrine administration at suitable intervals time before slaughtering or allowing birds to struggle freely just during slaughtering. Premortem glycolysis, occurring as a result death 1‐2 hr slaughtering, lowered pH meat caused toughness. Minimization more than 5 increased ultimate tenderness. The results indicate that value above 6.2 after an...
Carbapenem-resistant Enterobacterales (CRE) and Pseudomonas aeruginosa (CR-PA) producing metallo-β-lactamases (MBLs) cause severe nosocomial infections with no defined treatment. The combination of aztreonam (ATM) ceftazidime-avibactam (CZA) is a potential therapeutic option, but there approved, feasible testing method for use in clinical laboratories to assess the activity two antimicrobials combination. Here, we evaluate performance four ATM-CZA methods, as follows: broth disk elution...
We report the emergence of non-susceptibility to cefiderocol from a subpopulation Pseudomonas aeruginosa recovered patient without history exposure. Whole genome sequencing identified mutations in major iron transport pathways previously associated with uptake. Susceptibility testing should be performed before therapy siderophore cephalosporins.
Stenotrophomonas maltophilia causes high-mortality infections in immunocompromised hosts with limited therapeutic options. Many U.S. laboratories rely on commercial automated antimicrobial susceptibility tests (cASTs) and use CLSI breakpoints (BPs) for S. maltophilia. However, contemporary data these systems are lacking. We assessed performance of Vitek 2, MicroScan WalkAway, Phoenix relative to that reference broth microdilution trimethoprim-sulfamethoxazole (SXT), levofloxacin (LEV),...
Abstract Objective Multi-drug resistance (MDR) has notably increased in community acquired uropathogens causing urinary tract infections (UTIs), predominantly Escherichia coli . Uropathogenic E. causes 80% of uncomplicated UTIs, particularly pre-menopausal women. Considering this high prevalence and the potential to spread antimicrobial resistant genes, current study was conducted investigate presence clinically important strains Pakistani women having cystitis pyelonephritis. Women...
SUMMARY: A synthetic medium containing inorganic sources of N, P, Fe, S, Ca2+ and Mg2+, trace minerals essential vitamins was developed for anaerobic degradation cellulose to CH4. mixed culture obtained from sewage sludge grown in batch this degraded about 4 g l−1 week−1 produced 710 ml total gas per degraded. The contained between 51 56% CH4, the remainder being CO2. For maximum CH4 CO2, requirement salts other than HCO3 −, Fe S2- comparable that anaerobes. NH4 + or Na2CO3 at 20 24 mm 0·4...
SUMMARY– Tests made on pectoralis major muscles having post‐slaughter pH values ranging between 6.1–7.0, indicated that holding poultry meat at 30 and 37°C during the onset of rigor mortis caused toughness. This toughening effect high temperature appeared to occur when level dropped from a value about 6.3 its ultimate low adenosine triphosphate content below 40% initial concentration. Holding temperatures 10, 15 25°C mortis, or cooling 15°C before produced more tender meat. After completion...
SUMMARY In both breast and leg muscle from 6‐, 9‐, 12‐month‐old chickens, held for aging at 0°C, the buffer‐extractable nitrogen rapidly decreased after death during onset of rigor gradually increased to a maximum value post‐rigor aging. Changes in extractable occurred mainly as result changes solubility myofibrillar proteins. sarcoplasmic stroma protein fractions were small. nonprotein‐nitrogen fraction, some amino‐acid‐containing polymers removed mortis their interaction or aggregation...
SUMMARY Quantitative examination of chicken muscle proteins showed that protein extractability in both breast and leg decreased during frozen storage because loss solubility actomyosin fraction. This decrease accompanied a the sulfhydryl‐group content muscles myosin‐adenosinetriphosphatase activity. The stroma‐protein fraction remained unaffected, sarcoplasmic‐protein only after long storage. In non‐protein‐nitrogen fraction, amount free amino acids other protein‐breakdown products increased...
SUMMARY— A routine method for the extraction, chromatographic separation and ultraviolet spectrophotometric assay of ribonucleotides their derivatives in chicken muscle was developed to study changes ribonucleotide content from moment death until tenderness are essentially complete. In this method, mean error duplicate analyses ±2%, recoveries were within ±6% amounts added. Results indicated that formation inosinic acid occurred as a result breakdown adenosine triphosphate during period time...
SUMMARY Different buffer systems were compared for efficiency of extraction chicken muscle proteins, and a technique was developed routine fractionation estimation major protein fractions in one operation. KCl‐borate KCI‐phosphate buffers pH 7.3–7.5 r/2 = 1.0 gave maximum extraction, with reproducibility ± 22%. Protein showed that one‐year‐old meat, stroma‐myofibrillar‐, sarcoplasmic‐protein nitrogen respectively contributed 13, 42, 30% total breast muscle, 27, 30, 22% leg muscle. Results...
Treatment of serious infections due to multidrug-resistant (MDR) Pseudomonas aeruginosa remains a challenge, despite the introduction novel therapeutics. In this study, we report 2 extensively drug-resistant clinical isolates sequence type (ST) 309 P resistant all β-lactams, including combinations ceftolozane/tazobactam, ceftazidime/avibactam, and meropenem/vaborbactam.Isolates were sequenced using both short-read (Illumina) long-read technology identify resistance determinants,...
Abstract Objectives To characterize a blaCMY variant associated with ceftazidime/avibactam resistance from serially collected Escherichia coli isolate. Methods A patient an intra-abdominal infection due to recurrent E. was treated ceftazidime/avibactam. On Day 48 of therapy, MIC >256 mg/L identified abdominal drainage. Illumina and Oxford Nanopore Technologies WGS performed on serial isolates identify potential mechanisms. Site-directed mutants CMY β-lactamase were constructed amino...