A5049922796- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Microtubule and mitosis dynamics
- Genomics and Phylogenetic Studies
- Algal biology and biofuel production
- Advanced Nanomaterials in Catalysis
- Physiological and biochemical adaptations
- Nanoparticles: synthesis and applications
- Photosynthetic Processes and Mechanisms
- Marine and coastal ecosystems
- Enzyme Structure and Function
- Graphene and Nanomaterials Applications
- Enzyme Production and Characterization
- Polar Research and Ecology
- Chromium effects and bioremediation
- Isotope Analysis in Ecology
- Toxic Organic Pollutants Impact
- Environmental Toxicology and Ecotoxicology
- Urinary Tract Infections Management
- Bacteriophages and microbial interactions
- Genetic and Kidney Cyst Diseases
- Microbial Metabolic Engineering and Bioproduction
- Methane Hydrates and Related Phenomena
- Enzyme Catalysis and Immobilization
- Protein Structure and Dynamics
Università di Camerino
2016-2025
University of Bern
2019
National Academies of Sciences, Engineering, and Medicine
2016
Institute of Biomedical Technologies
2011
Laboratoire d'Enzymologie et Biochimie Structurales
2002
Oxidative stress is a particularly severe threat to Antarctic marine polar organisms because they are exposed high dissolved oxygen and intense UV radiation. This paper reports the features of three superoxide dismutases from psychrophilic ciliate Euplotes focardii that faces two environmental challenges, oxidative low temperature. Two out these Cu,Zn (named Ef-SOD1a Ef-SOD1b) one belongs Mn-containing group (Ef-SOD2). Ef-SOD1s Ef-SOD2 differ in their evolutionary history, expression overall...
Abstract Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span diversity of eukaryotic tree life. However, genetic tractability has been limited to a few species, which do not represent or environmentally relevant taxa. Here, we report on development tools range protists primarily from marine environments. We present evidence for foreign DNA delivery and expression 13 species never before transformed advancement eight other as...
The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used new Pseudomonas strain isolated from consortium associated with the Antarctic marine ciliate Euplotes focardii. After incubation cultures 1 mM AgNO3 at 22 °C, obtained AgNPs within 24 h. Scanning electron (SEM) transmission microscopy (TEM) revealed spherical polydispersed size...
In the last decade, metal nanoparticles (NPs) have gained significant interest in field of biotechnology due to their unique physiochemical properties and potential uses a wide range applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, viable strategy alternative chemical physical approaches. Herein, original efficient route for microbial copper NPs bacterial strains newly isolated from Antarctic consortium is described. UV-visible spectra showed...
Cold environments are populated by organisms able to contravene deleterious effects of low temperature diverse adaptive strategies, including the production ice binding proteins (IBPs) that inhibit growth crystals inside and outside cells. We describe properties such a protein (EfcIBP) identified in metagenome an Antarctic biological consortium composed ciliate Euplotes focardii psychrophilic non-cultured bacteria. Recombinant EfcIBP can resist freezing without any conformational damage is...
Abstract Objective Multi-drug resistance (MDR) has notably increased in community acquired uropathogens causing urinary tract infections (UTIs), predominantly Escherichia coli . Uropathogenic E. causes 80% of uncomplicated UTIs, particularly pre-menopausal women. Considering this high prevalence and the potential to spread antimicrobial resistant genes, current study was conducted investigate presence clinically important strains Pakistani women having cystitis pyelonephritis. Women...
Abstract Background The scope of this work is to build a Machine Learning model able predict patients risk contract multidrug resistant urinary tract infection (MDR UTI) after hospitalization. To achieve goal, we used different popular tools. Moreover, integrated an easy-to-use cloud platform, called DSaaS ( Data Science as Service ), well suited for hospital structures, where healthcare operators might not have specific competences in using programming languages but still, they do need...
To survive in cold environments, psychrophilic organisms produce enzymes endowed with high specific activity at low temperature. The structure of these is usually flexible and mostly thermolabile. In this work, we investigate the structural basis adaptation a GH42 β-galactosidase from Marinomonas ef1. This enzyme couples astonishing robustness for protein, it retains 23% its highest 5 °C stable several days 37 even 50 °C. Phylogenetic analyses indicate close relationship thermophilic...
In this study, we report on the synthesis of silver nanoparticles (AgNPs) achieved by using three bacterial strains Rhodococcus, Brevundimonas and Bacillus as reducing capping agents, newly isolated from a consortium associated with Antarctic marine ciliate Euplotes focardii. After incubation these bacteria 1 mM solution AgNO3 at 22 °C, AgNPs were synthesized within 24 h. Unlike Rhodococcus Bacillus, reduction Ag+ into Ag0 has never been reported for strain. The maximum absorbances in UV-Vis...
ABSTRACT The α-amylases are endo-acting enzymes that hydrolyze starch by randomly cleaving the 1,4-α- d -glucosidic linkages between adjacent glucose units in a linear amylose chain. They have significant advantages wide range of applications, particularly food industry. eukaryotic α-amylase isolated from Antarctic ciliated protozoon Euplotes focardii ( Ef Amy) is an alkaline enzyme, different most characterized so far. Furthermore, Amy has characteristics psychrophilic α-amylase, such as...
We report the characterization of bacterial consortium associated to Euplotes focardii, a strictly psychrophilic marine ciliate that was maintained in laboratory cultures at 4 °C after its first isolation from Terra Nova Bay, Antarctica. By Illumina genome analyser, we obtained 11,179 contigs potential prokaryotic origin and classified them according NCBI's attributes table. The majority these sequences correspond either Bacteroidetes (16 %) or Proteobacteria (78 %). latter were dominated by...
We isolated a novel bacterial strain from prokaryotic consortium associated to the psychrophilic marine ciliate Euplotes focardii, endemic of Antarctic coastal seawater. The 16S rDNA sequencing and phylogenetic analysis revealed close evolutionary relationship bacterium Marinomonas sp. BSw10506 sub antarctic polaris. named this new ef1. optimal growth temperature in LB medium was 22 °C. Whole genome showed reduced gene loss limited regions encoding for transposases. Additionally, five...
Abstract The macronuclear (MAC) genomes of ciliates belonging to the genus Euplotes species are comprised numerous small DNA molecules, nanochromosomes, each typically encoding a single gene. These responsible for all gene expression during vegetative cell growth. Here, we report analysis MAC genome from Antarctic psychrophile focardii. Nanochromosomes containing bacterial sequences were not found, suggesting that phenomena horizontal transfer did occur recently, even though this ciliate has...
Marine microorganisms have been demonstrated to be an important source for bioactive molecules. In this paper we report the synthesis of Ni nanoparticles (NiSNPs) used as reducing and capping agents five bacterial strains isolated from Antarctic marine consortium: Marinomonas sp. ef1, Rhodococcus Pseudomonas Brevundimonas Bacillus ef1. The NiSNPs were characterized by Ultraviolet–visible (UV–vis) spectroscopy, Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), X-ray...
<title>Abstract</title> Alternative splicing (AS) of introns is a key mechanism contributing to proteomic diversity. It enables the generation multiple mRNAs variants from single gene sequence, which are subsequently translated into distinct protein isoforms. Intron retention (IR) specific type AS in remain unspliced mature mRNA. The process intron regulated by cis-regulatory elements that recruit small non-coding RNAs and heterogeneous nuclear ribonucleoproteins (hnRNP), collectively...
Abstract Tubulin dimers of psychrophilic eukaryotes can polymerize into microtubules at 4°C, a temperature which from mesophiles disassemble. This unique capability requires changes in the primary structure and/or post‐translational modifications tubulin subunits. To contribute to understanding mechanisms responsible for microtubule cold stability, here we present computational structural analysis based on molecular dynamics (MD) and experimental data three β‐tubulin isotypes, named EFBT2,...
Glutathione peroxidases (GPxs) form a broad family of antioxidant proteins essential for maintaining redox homeostasis in eukaryotic cells. In this study, we used an integrative approach that combines bioinformatics, molecular biology, and biochemistry to investigate the role GPxs reactive oxygen species detoxification unicellular model organism Tetrahymena thermophila. Both phylogenetic mechanistic empirical analyses provided indications about evolutionary relationships among GPXs...
Marinomonas sp. ef1, in 1% (v/v) of diesel and biodiesel was able to reproduce itself, confirmed by the OD 600 curve; decrease COD parameter degradation diesel; case bacteria synthesized a secondary fluorescent compound.