A5054369721- Sphingolipid Metabolism and Signaling
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Receptor Mechanisms and Signaling
- RNA Interference and Gene Delivery
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Lipid metabolism and biosynthesis
- Phosphodiesterase function and regulation
RIKEN Center for Brain Science
2023
RIKEN
1997-1998
Yokohama National University
1998
Kyoto University
1993
Activation of Gq-type G protein-coupled receptors (GPCRs) gives rise to large cytosolic Ca2+ elevations in astrocytes. Previous vitro and vivo studies have indicated that astrocytic are closely associated with diameter changes the nearby blood vessels, which astrocytes enwrap their endfeet. However, causal relationship between vessel has been questioned, as mice diminished signaling show normal sensory hyperemia. We addressed this controversy by imaging cortical vasculature while...
A complementary DNA encoding the key subunit of human N-methyl-D-aspartate (NMDA) receptor (NMDAR1) has been cloned using a probe derived from rat NMDAR1 cDNA. The cDNA encodes 938-amino acid protein, which shows 99% amino homology with counterpart. Of 7 938 acids are different, three occur in region signal peptide and others extracellular amino-terminal domain preceding 4 putative transmembrane segments. Expression Xenopus oocytes demonstrated that single protein encoded by possesses...
Human genetics strongly support the involvement of synaptopathy in psychiatric disorders. However, trans-scale causality linking synapse pathology to behavioral changes is lacking. To address this question, we examined effects synaptic inputs on dendrites, cells, and behaviors mice with knockdown SETD1A DISC1, which are validated animal models schizophrenia. Both exhibited an overrepresentation extra-large (XL) synapses, evoked supralinear dendritic somatic integration, resulting increased...
Abstract Ceramide glucosyltransferase (EC 2.4.1.80) catalyzcs the first glycosylation step of glycosphingolipid (GSL) synthesis, transfer glucose from UDP‐Glucose to hydrophobic ceramide and generate gtucosylceramide (GlcCer). We have chined mouse cDNA a brain library by PCR based homology cloning. The nucleotide sequence determination revealed that ceramidc encodes 394 amino acids with calculated molecular mass 45 kDa. acid showed 98% identity human sequence. Homology searches against...
The gene encoding a flavodoxin of Desulfovibrio vulgaris (Miyazaki F) was cloned, and overexpressed in Escherichia coli. A 1.6-kbp DNA fragment, isolated from D. by double digestion with SalI EcoRI, contained the its regulatory region. An expression system for under control T7 promoter constructed E. purified protein soluble exhibited characteristic visible absorption spectrum. HPLC analysis recombinant revealed presence an identical FMN to that found native flavodoxin, dissociation constant...