A5007252252- DNA Repair Mechanisms
- Cancer Cells and Metastasis
- Skin Protection and Aging
- melanin and skin pigmentation
- Nonmelanoma Skin Cancer Studies
- Cutaneous Melanoma Detection and Management
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- Cell death mechanisms and regulation
- Infectious Diseases and Mycology
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- CAR-T cell therapy research
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Silk-based biomaterials and applications
- Cancer Genomics and Diagnostics
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- Cancer and Skin Lesions
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
Elbe Kliniken Stade-Buxtehude
2020-2025
University Medical Center Hamburg-Eppendorf
2008-2020
Universität Hamburg
2008-2020
Humanized tumor mice (HTM) allow for preclinical cancer treatment studies of breast (BC) under human-like conditions. This study utilized HTM the first time to investigate potential miRNA biomarker candidates response in sera and extracellular vesicles (EVs), following X-irradiation atezolizumab (anti-PD-L1) treatment. We identified changes human-specific miRNAs (miR-23b-3p miR-155-5p) after irradiation anti-PD-L1 HTMs with human epidermal growth factor receptor 2 positive (HER2+ BC)...
Dermal stem cells (DSCs), which are progenitor of melanocytes, isolated from human foreskin and cultivated as mixed cultures containing both DSCs fibroblasts in varying proportions. These contaminating may have an impact on the results experimental studies a serious limitation for certain applications. The aim present study was to purify or enrich DSCs—an indispensable step towards future investigations. Applying different methods, we demonstrated that highly enriched with good recovery rate...
Solar radiation is the main risk factor for cSCC development, yet it unclear whether progression of promoted by solar in same way as initial tumorigenesis. Additionally, role miRNAs, which exert crucial functions various tumors, needs to be further elucidated context and connection radiation. Thus, we chronically irradiated five cell lines (Met-1, Met-4, SCC-12, SCC-13, SCL-II) with a custom-built irradiation device mimicking spectrum (UVB, UVA, visible light (VIS), near-infrared (IRA))....
Melanoma treatment has improved significantly with the development of immune checkpoint inhibition (ICI), which greatly enhanced survival rates patients metastatic melanoma. However, a significant number do not respond well to ICI and experience progression. This highlights critical need for practical means track melanoma patients' response ICI. To address this issue, patterns circulating miRNAs were studied in liquid biopsies patients. These have potential provide essential information...
Here, we present a modified in vitro end-joining (EJ) assay to quantify EJ capacity, accuracy as well pathway switch alternative (Alt-EJ) or single strand annealing (SSA). A novel transformation was established specifically measure circular repair products, which correlate with classical efficiency. The validated using EJ-deficient mammalian cell lines (Ku80, DNA-PKcs, LigIV, XRCC4 mutants). Alt-EJ and SSA seen exclusively Ku-deficient cells. Circular product formation correlated survival...
Purpose: Melanom therapy has been revolutionized by immune checkpoint inhibition(ICI)Inhibitors of PD-1-, PD-L1 and CTLA-4 checkpoints led to tremendous improvement survival patients with metastatic melanoma. However, a considerable portion does not respond shows progress under ICI. Thus, there is an urgent need for clinically applicable biomarkers monitor patients’ capabilities ICI treatment. Therefore, we monitored expression patterns circulating miRNAs in plasma melanoma characterize...