The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an emergent infectious pathogen causing disease called corona virus 2019 (COVID-19). Virus transmission may occur by contact, droplet, airborne or via contaminated surfaces. In efforts to effectively control the COVID-19 outbreak, world health organization (WHO) and Saudi Ministry of Health (MOH) have advised public practice protective measures reduce incidence infection. These include hand washing, wearing masks gloves...

Background Finding new effective substances to develop drugs against cancer or prevent it remains a big challenge facing scientists and clinicians. Objectives In such an issue, we tested the anti-tumoral effect of Scorzonera undulata extracts on MCF7 cells. Materials Methods Methanolic S. roots (RSU) aerial parts (ASU) from Ha’il region were analyzed by HPLC. Their DPPH scavenging, ferric-reducing antioxidant power (FRAP) total potentials evaluated. The MTT test was used determine their...

ZnO nanoparticles (NPs) were prepared by green synthesis using plant leaf extraction of Ochradenus baccatus and characterized XRD, FESEM, HRTEM, Raman spectroscopy techniques. Since elevated CO levels have been associated with inflammatory conditions, cardiovascular diseases, respiratory disorders the methane gas primarily produced gut microbiota linked to gastrointestinal other abnormal in breath samples, applied for sensor fabrication. Thus, sensors fabricated investigated CH4, H2, CO, NO2...

Abstract INTRODUCTION Genome‐wide association studies link susceptibility to late‐onset Alzheimer's disease (LOAD) with EphA 1. Sequencing identified a non‐synonymous substitution P460L as LOAD risk variant. Other Ephs regulate vascular permeability and immune cell recruitment. We hypothesized that dysregulates EphA1 receptor activity impacts neuroinflammation. METHODS EphA1/P460L was assayed in isogenic Human Embryonic Kidney (HEK) cells. Soluble (sEphA1/sP460L) reverse signaling brain...

Glutamic acid decarboxylase-65 (GAD(65)) is an immunological marker of type 1 autoimmune diabetes. High titre autoantibodies against GAD(65) (GAD(65)Abs) have also been detected in some other diseases. In search a potential diabetes, vitro was modified by hydroxyl radical followed the study structural and conformational perturbed protein different spectroscopic techniques (UV, fluorescence CD) thermal denaturation profile. Binding studies circulating from diabetic groups (type 2) with native...

Molluscum contagiosum virus (MCV) is a significant but underreported skin pathogen for children and adults. Seroprevalence studies can help establish burden of disease. Enzyme linked immunosorbent assay (ELISA) based have been published Australian Japanese populations the results indicate seroprevalences between 6 22 percent in healthy individuals, respectively. To investigate seroprevalence Europe, we developed recombinant ELISA using truncated MCV virion surface protein MC084 (V123-R230)...

Healthcare workers (HCWs) are the backbone of healthcare system and a skilled healthy workforce is vital during health crisis, such as present coronavirus disease 2019 (COVID-19) pandemic. at higher risk exposure to transmission severe acute respiratory coronavirus-2 (SARS-CoV-2). Hence, HCWs should possess good knowledge attitudes toward COVID-19 protect not only themselves but also their colleagues, families larger community. Thus, current study aims assess practice protective measures by...

Aging causes gradual changes in free radicals, antioxidants, and immune-imbalance the elderly. This study aims to understand links among aging, gluco-oxidative stress, autoantibodies asymptomatic individuals. In vitro glycation of human serum albumin (Gly-HSA) induces appreciable biochemical changes. Significant inhibition advanced end products (AGEs) formation was achieved using garlic extract (53.75%) epigallocatechin-3-gallate from green tea (72.5%). Increased amounts carbonyl content...

COVID-19 infection is associated with elevated oxidative stress, systemic hyper-inflammatory responses, endothelial dysfunction, and red blood cell membrane deformability. Nigella sativa extract widely used in alternative complementary medicine systems a large population, due to its highly therapeutic, economic, natural, safe nature. The aim of this study was evaluate the effect N. on hemolysis, proteolysis, glycation through vitro studies, as well find out anti-viral potential against...

<b><i>Background:</i></b> Depression and smoking contribute to the prognosis of autoimmune rheumatoid arthritis (RA). Advanced glycation end products (AGEs) are also detected in RA patients. This study correlates patients with various levels depression a history through detection antibodies against AGEs proteins. <b><i>Methods:</i></b> Sixty subjects were selected divided into 4 groups based on their habits. The division was as follows: group I...

Garlic has been reported to inhibit protein glycation, a process that underlies several disease processes, including chronic complications of diabetes mellitus. Biophysical, biochemical, and molecular docking investigations were conducted assess anti-glycating, antioxidant, structural protection activities garlic. Results from spectral (UV fluorescence) circular dichroism (CD) analysis helped ascertain conformation secondary structure against glycation significant extent. Further, garlic...

Cancer management is highly dependent on the immune status of patient. During COVID-19 pandemic, a large number people suffered from anxiety and depression, especially cancer patients. The effect depression breast (BC) prostate (PC) patients, during pandemic has been analyzed in this study. Levels proinflammatory cytokines (IFN-γ, TNF-α, IL-6) oxidative stress markers malondialdehyde (MDA) carbonyl content (CC) were estimated patients' serum samples. Serum antibodies against vitro hydroxyl...

Controlled non-enzymatic glycation reactions are common under normal physiological conditions. However, during elevated blood glucose conditions, the accelerated, leading to formation of toxic compounds such as advanced end products (AGEs). Several natural now being investigated protective agents against preserve protein structure and functions.

This study investigates unique and specific properties of the lanthanum based zinc molybdate nanocomposite material. The material was synthesized using a simple low cost “co-precipitation” method. nano materials had structural, vibrational morphological properties, determined by X-ray diffraction, fourier-transform infrared spectroscopy field emission scanning electron microscopy. nanomaterial characterised range techniques. diffraction used to determine particle size 65.7 nm disc shaped...

Background Selective cancer cell recognition is the most challenging objective in targeted delivery of anti-cancer agents. Extruded specific membrane coated nanoparticles, exploiting potential homotypic binding along with certain protein-receptor interactions, have recently proven to be method choice for drugs. Prediction selective targeting efficiency encapsulated nanoparticles (CCMEN) critical aspect selecting this strategy as a delivery. Materials and methods A probabilistic model based...

Leprosy is a chronic infectious disease caused by bacillus, Mycobacterium leprae. According to official data from 139 countries in the 6 WHO Regions, there were 127558 new leprosy cases worldwide 2020. mainly affects skin, peripheral nerves, mucosa of upper respiratory tract, and eyes. If this left untreated, can harm limbs, eyes, skin permanently. The curable with multidrug therapy. Over period time leprae has become resistant these drugs. Therefore, therapeutic molecules are warranted....

Coronavirus disease 2019, caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is a communicable transmitted through route and bodily contact. The severity of infection mortality rate COVID-19 cases was significantly high in initial stages pandemic. This study aims to investigate hematological profile survivors non-survivors.This single-center retrospective study. A total 108 hospitalized patients with laboratory-confirmed at East Jeddah Hospital between April August...

Zinc oxide (ZnO) nanoparticles (NPs) are increasingly used in the diagnosis and prevention of various human infections diseases. The multi-functional nature these together with characteristics such as low toxicity biodegradability, allow their use a slew products applications ranging from biomedical, food supplement industries, therapeutics biosensors. ZnO exhibits biomimetic properties enabling biomedical applications, including alternatives to pre-existing antibiotics. This study discusses...

Human Vg9/Vδ2 T cells (γδ cells) are immune surveillance both in innate and adaptive immunity a possible target for anticancer therapies, which can induce responses variety of cancers. Small non-peptide antigens such as zoledronate do activation expansion vitro. It is evident that adoptive cancer large numbers functional needed into patients. Hence, optimization methods needs to be carried out the efficient these cells. Standardization peripheral blood mononuclear (PBMCs) isolation was...

Advanced stage cancers are aggressive and difficult to treat with mono-therapeutics, substantially decreasing patient survival rates. Hence, there is an urgent need develop unique therapeutic approaches cancer superior potency efficacy. This study investigates a new approach potent combinational therapy advanced leukemia. Biologically active α-amino amide analogs (RS)-N-(2-(cyclohexylamino)-2-oxo-1-phenylethyl)-N-phenylpropiolamide (α-AAA-A)...