A5028034286- Vestibular and auditory disorders
- Hearing, Cochlea, Tinnitus, Genetics
- Inflammatory mediators and NSAID effects
- Rheumatoid Arthritis Research and Therapies
- Hearing Loss and Rehabilitation
- Systemic Lupus Erythematosus Research
- Estrogen and related hormone effects
- Ear Surgery and Otitis Media
- Salivary Gland Disorders and Functions
- Noise Effects and Management
- Monoclonal and Polyclonal Antibodies Research
- Peroxisome Proliferator-Activated Receptors
- Salivary Gland Tumors Diagnosis and Treatment
- Cerebral Venous Sinus Thrombosis
- Cancer, Lipids, and Metabolism
- Glaucoma and retinal disorders
- Ear and Head Tumors
- Autoimmune and Inflammatory Disorders Research
- Eicosanoids and Hypertension Pharmacology
- Systemic Sclerosis and Related Diseases
- Otitis Media and Relapsing Polychondritis
- Sphingolipid Metabolism and Signaling
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- NF-κB Signaling Pathways
Kitasato University
2016-2025
Ijinkai Takeda General Hospital
2024-2025
Hyogo University
2025
Hyogo Medical University
2013-2024
The Memory Clinic
2024
University College London
2023
St. Marianna University School of Medicine
2021
Yukioka Hospital
2020
Ministry of Health Labour and Welfare
2012-2018
Kyoto Prefectural University of Medicine
1995-2016
High levels of immunoreactive cyclooxygenase (Cox; prostaglandin H synthase) are present in synovia from patients with rheumatoid arthritis (RA). We now show that the recently identified inducible isoform Cox, Cox-2, is expressed RA. To further explore modulation Cox isoforms RA synovial tissues, we examined expression and Cox-1 -2 explant cultures cultured fibroblast-like cells (synoviocytes). Immunoprecipitation vitro labeled proteins Western blot analysis demonstrated presence both under...
Corticotropin-releasing hormone (CRH) functions as a regulator of the hypothalamic-pituitary-adrenal axis and coordinator stress response. CRH receptors exist in peripheral sites immune system, promotes several vitro. The effect systemic immunoneutralization was tested an experimental model chemically induced aseptic inflammation rats. Intraperitoneal administration rabbit antiserum to caused suppression both inflammatory exudate volume cell concentration by approximately 50 60 percent....
Peroxisome proliferator–activated receptors (PPARs) are members of the nuclear hormone receptor superfamily and have a dominant regulatory role in adipocyte monocyte differentiation. PPAR-γ agonists also negative regulators macrophage activation modulatory effects on tumorigenesis. In this study we demonstrate that synovial tissue localized expression patients with rheumatoid arthritis (RA). We detected markedly enhanced macrophages, as well modestly lining layer, fibroblasts, endothelial...
Cyclooxygenase (COX), or prostaglandin (PG) H synthase, plays a role in inflammatory diseases, but very limited data exist on the regulation of COX vivo. We, therefore, studied vivo expression synovia from patients with rheumatoid arthritis (RA) and osteoarthritis (OA), as well joints rats streptococcal cell wall (SCW) adjuvant arthritis. Extensive intense intracellular immunostaining, which correlated extent intensity mononuclear infiltration, was observed cells throughout RA synovia....
BACKGROUND Nonsteroidal antiinflammatory drugs inhibiting cyclooxygenase (COX) enzyme activity in both its constitutive (COX-1) and inducible (COX-2) isoforms were shown also to inhibit the development of colon carcinoma animal models. COX-2 is an inducer angiogenesis new blood vessels. The expression COX-1 prostate tissues from patients with was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR) immunohistochemistry. METHODS Tumor specimens obtained 28 (PC)...
Corticotropin-releasing hormone (CRH), the principal regulator of hypothalamic-pituitary-adrenal axis, is also secreted in peripheral inflammatory sites, where it acts as a local proinflammatory agent. Arthritis-susceptible LEW/N rats have profoundly deficient hypothalamic CRH responses to stimuli and other stressors. Arthritis-resistant F344/N rats, on hand, robust increase response same stimuli. Contrasting with these responses, we now show that expression markedly increased joints...
Sphingosine kinase (Sphk) enzymes are important in intracellular sphingolipid metabolism as well the biosynthesis of sphingosine 1-phosphate (S1P), an extracellular lipid mediator. Here, we show that Sphk1 is expressed and required for small intestinal tumor cell proliferation ApcMin/+ mice. Adenoma size but not incidence was dramatically reduced ApcMin/+Sphk−/− Concomitantly, epithelial polyps significantly attenuated, suggesting regulates adenoma progression. Although S1P receptors (S1P1R,...
The synovium from patients with rheumatoid arthritis (RA) and LEW/N rats streptococcal cell wall (SCW) arthritis, an experimental model resembling RA, is characterized by massive proliferation of synovial connective tissues invasive destruction periarticular bone cartilage. Since heparin binding growth factor (HBGF)-1, the precursor acidic fibroblast (FGF), a potent angiogenic polypeptide mitogen for mesenchymal cells, we sought evidence that it was involved in pathology RA SCW arthritis....
Abstract Objective Sphingosine 1‐phosphate (S1P) is involved in various pathologic conditions and has been implicated as an important mediator of angiogenesis, inflammation, cancer, autoimmunity. This study was undertaken to examine the role S1P/S1P 1 signaling pathogenesis rheumatoid arthritis (RA). Methods We examined S1P messenger RNA (mRNA) protein levels RA synoviocytes MH7A cells by reverse transcriptase–polymerase chain reaction Western blotting. also performed immunohistochemistry...
To investigate the duration of remission and low disease activity (LDA) after cessation tocilizumab (TCZ) treatment in rheumatoid arthritis patients who showed or LDA as assessed by DAS28 response to preceding TCZ monotherapy, explore factors contributing prolonged efficacy duration.Disease was monitored for 56 weeks. The rate continued estimated Kaplan-Meier curves.A total 187 were eligible. At baseline this study, median 7.8 years, period 4.0 years 1.5. at 52 weeks 13.4 % according...
Corticotropin releasing hormone (CRH) is a key in integrated response to stress, acting as the major regulator of hypothalamic-pituitary-adrenal axis. Recently, local production CRH has been detected normal human colonic enterochromaffin cells. locally secreted granulomatous and arthritic tissues rats humans, where it seems act proinflammatory agent. To find out if present patients with ulcerative colitis, this study examined expression peptide large bowel colitis. Colonic colitis obtained...
To determine whether the frequencies of CD4+CD25+ regulatory T cells (T Reg) were related to immune status in HIV-infected patients.Peripheral blood CD4 T-cell populations examined for T-helper 1 (Th1), 2 (Th2), and Reg by intracellular staining interferon (IFN)-gamma interleukin (IL)-4, surface CD25, respectively. The immunoregulatory properties assessed measurement inhibitory effects isolated on CD4+CD25- proliferation.Isolated from both patients healthy controls strongly expressed CD45RO,...
DNA fragments were isolated from DNA/anti-DNA antibody immune complexes of systemic lupus erythematosus. Hybridization experiments indicated that originates human nuclear DNA. Two classes identified by polyacrylamide gel electrophoresis. The m.w. small was 25,000 with 30 to 40 base pairs, and large 100,000 150 pairs. average guanine-cytosine (G-C) content in the 55% total bases, 45%. Compared 38% G-C DNA, antigen is rich content. Such unusually high can alter structure, which may serve as...
Atopic dermatitis (AD) is a common inflammatory skin disease of unknown etiology. Cutaneous infection with microbes such as Staphylococcus aureus and/or cleansing detergent exacerbates clinical AD. Here, we generated an AD animal model by destroying barrier function and subsequent topical application protein A from S. (SpA). NC/Nga mice, which genetically have reduced function, BALB/c mice having intact were susceptible to this combination developed severe moderate AD, respectively,...
Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease. Certain HLA-DRB1 "shared-epitope" alleles are reported to be positively associated with increased RA susceptibility, whereas some of the other may negatively associated. However, studies on latter rare. Here, we focus protective effects DRB1 in Japanese patients an association study. Relative predispositional (RPE) were analyzed by sequential elimination carriers each allele strongest association. The investigated...
Using a large-scale nationwide survey database, we investigated the epidemiological characteristics for idiopathic SSNHL in Japan.The subjects this analysis were patients registered Japanese multicentre database between April 2014 and March 2016. A total of 3419 clinical obtained. Several factors associated with severity hearing impairment prognosis then investigated. Statistical was performed to clarify prognosis.There significant correlations loss diabetes mellitus, kidney disease, past...
Idiopathic inflammatory myopathies (IIMs) are a heterogeneous group of rare autoimmune diseases in which both genetic and environmental factors play important roles. To identify IIM including polymyositis, dermatomyositis (DM) clinically amyopathic DM (CADM), we performed the first genome-wide association study for an Asian population.We genotyped tested 496 819 single nucleotide polymorphism using 576 patients with 6270 control subjects. We also examined causal mechanism disease-associated...
Recent studies have demonstrated that peroxisome proliferator activator-receptors (PPAR)-gamma is expressed in some cancer cells such as breast, lung, and gastric cancer, its ligand induces growth arrest of these through apoptosis. However, the expression localization PPARs prostate not been examined. In this study, was investigated human (PC), prostatic intraepithelial neoplasia (PIN), benign hyperplasia (BPH), normal (NP) tissues.Tumor specimens were obtained from 156 patients with PC, 15...
Abstract Recent studies have demonstrated that peroxisome proliferator activator‐receptors(PPAR)‐γ is expressed in various cancer tissues and its ligand induces growth arrest of these cells through apoptosis. In our study, we investigated the expression PPAR‐α, β γ human bladder tumor (BT) normal (NB) as well effects PPAR‐γ ligands. Specimens were obtained from 170 patients with BT 20 NB. The expressions using RT‐PCR immunohistochemical methods. We also inhibitory effect ligands on...
Transin is a neutral metalloproteinase initially isolated from malignantly transformed rat fibroblasts and subsequently shown to be homologous human stromelysin. We performed Northern blot analysis on synovial tissue specimens Lewis rats with proliferative invasive streptococcal cell wall (SCW) arthritis. mRNA was present in abundance, as the of c-myc oncogene, which associated cellular proliferation. Immunohistochemical staining synovia chronic SCW arthritis showed high-level transin...
A low molecular weight DNA fragment was isolated from DNA/anti-DNA antibody immune complexes found in patients with active systemic lupus erythematosus (SLE). Total sera were treated 40% saturated ammonium sulfate to isolate gamma-globulin fraction and phenolized partition proteins nucleic acids. After being RNAase, the sample labeled at 5' end 32P-phosphate electrophoresed an 8% polyacrylamide gel, which dried autoradiographed. The that migrated positions of between 20,000 28,000...
The relationship between clinical activity of systemic lupus erythematosus (SLE) and molecular sizes DNA fragments isolated from DNA/anti-DNA antibody immune complexes were examined. Among sera twenty-eight patients with SLE examined, three different identified, namely, m.v. 25,000 30 to 50 base pairs (bp); m.w. 100,000 150 200 bp; 200,000 300 bp. On the basis fragments, we can divide into four groups. first group contained predominantly 25,000. second 100,000. third 200,000. fourth both A...