Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death in world. Immunological analysis tumor microenvironment (immunoscore) shows great promise for improved prognosis and prediction response to immunotherapy. However, exact immune composition NSCLC remains unclear. Here, we used flow cytometry characterize infiltrate tumors, non-cancerous tissue, regional lymph node, blood. The cellular identity >95% all CD45+ cells was determined. Thirteen distinct types were...

Tumor-associated macrophages may either promote or suppress tumor growth depending on their activation status. Interferon-γ (IFN-γ) has been identified as a key factor for inducing tumoricidal M1 phenotype in macrophages. However, it remains unclear whether IFN-γ is sufficient if additional stimuli are required. Here, we tested and panel of toll-like receptor (TLR) agonists the ability to activate murine toward phenotype. The following TLR ligands were used: TLR1/TLR2 agonist Pam3CSK4,...

Despite therapeutic advances multiple myeloma remains largely incurable, and novel concepts are needed. The Hsp90-chaperone is a reasonable target, because it maintains oncogenic signaling of deregulated pathways. However, in contrast to promising preclinical results, only limited clinical efficacy has been achieved through pharmacological Hsp90 inhibition. Because Hsp70 described interact functionally with the Hsp90-complex, we analyzed suitability Hsp72 Hsp73 as potential additional target...

Triggering or enhancing antitumor activity of tumor-associated macrophages is an attractive strategy for cancer treatment. We have previously shown that the cytokine interferon- (IFN-), a type II IFN, could synergize with Toll-like receptor (TLR) agonists induction M1 macrophages. However, toxicity IFN- limits its clinical use. Here, we investigated whether less toxic I IFNs, IFN- and IFN-, potentially replace measured in vitro ability IFNs to TLR transcription inducible nitric oxide...

Equine piroplasmosis (EP) is caused by

Summary Three informative pig F 2 families based on European Wild Boar (W), Meishan (M) and Pietrain (P) crosses have been used for genome‐wide linkage quantitative trait loci (QTL) analysis. Altogether 129 microsatellites, 56 type I 46 definitions (specific to growth, fattening, fat deposition, muscling, meat quality, stress resistance body conformation) were included in the study. In maps of M × P, W P families, average spacing markers 18.4, 19.7 18.8 cM, numbers meioses 582, 534 625,...

We conducted a severe acute respiratory syndrome coronavirus 2 antibody seroprevalence study among >2,000 domestic cats from 4 countries during the first disease wave in Europe. found 4.4% using virus neutralization test and 4.3% receptor-binding domain ELISA, demonstrating probable human-to-cat transmission.

Hepatozoon canis is a protozoal agent that known to be transmitted by oral uptake of H. canis-infected Rhipicephalus sanguineus sensu lato ticks in dogs. Vertical transmission has only been described once study evaluating dogs from Japan. The aim this was investigate the parasitological status puppies bitch had tested positive for spp. prior giving birth.

Anaplasma phagocytophilum is an obligate intracellular bacterium that causes granulocytic anaplasmosis in domestic animals, wildlife, and humans primarily transmitted by ticks of the Ixodes persulcatus complex. This retrospective study aims to determine percentages dogs tested positive for A. Germany. It included results direct (polymerase chain reaction [PCR]) indirect (immunofluorescence antibody test [IFAT], antibody-enzyme-linked immunosorbent assay [ELISA]) detection methods performed...

Canine vector-borne infections have gained importance in Germany due to growing tourist traffic and an increased import of dogs from abroad. Endemic regions for pathogens such as Leishmania infantum, Hepatozoon canis, Ehrlichia Anaplasma platys Dirofilaria spp. are the Mediterranean area southeastern Europe. Babesia species phagocytophilum present all over The objective this retrospective study was evaluate prevalence imported defined endemic countries Europe.Medical records laboratory test...

Abstract Giardia duodenalis is a relevant gastrointestinal protozoan pathogen of humans and animals. This species complex consists eight genetically different assemblages. Assemblages A B are pathogenic to pets, thus confer zoonotic potential. The risk transmission has been controversially discussed. aim this monocentric cross‐sectional pilot study was investigate G. assemblages in pets living common households Berlin/Brandenburg (Germany). Samples from dogs, cats sharing the same were...

Abstract Background Vector‐borne diseases are of increasing importance in Germany. Since 2015, autochthonous cases have been increasingly documented Berlin/Brandenburg. Objectives Describe Babesia canis infection the Berlin/Brandenburg region. Animals Forty‐nine dogs with B. infection. Methods Evaluation history, clinical signs, laboratory abnormalities, treatment, and outcome. Results Dogs were presented between March August (9) September January (40) years 2015‐2021. Historical findings...

Summary The genetic diversity between European Wild Boar, Pietrain and Meishan their crossbred generations are described on the basis of performance traits for growth, carcass composition meat quality. Using results 1333 pigs, highest growth rates were found in generations, indicating positive heterosis effects. In composition, high content is associated with fat alleles. Weights organs greatest weight head was Meishan. Meat quality stress resistance parameters point to superior gene effects...

Summary Three F 2 families have been established for QTL mapping by crossing genetically divergent Wild boar, Meishan and Pietrain pigs. More than 900 animals are typed a large number of marker loci recorded over 100 quantitative traits. In first analysis QTLs on chromosome 6 mapped in the region ryanodine receptor gene. Also several traits were significantly associated with different GH haplotyes. Zusammenfassung Einsatz von Markergenen zur Kartierung quantitativer Merkmale Wildschwein, und...

The attenuated live vaccine strain bacille Calmette–Guérin (BCG) is currently the only available against tuberculosis (TB), but largely ineffective adult pulmonary TB, most common disease form. This in part due to BCG’s ability interfere with host innate immune response, a feature that might be targeted enhance potency of this vaccine. Here, we investigated chitosan-based nanoparticles (pIC-NPs) containing polyinosinic–polycytidylic acid (poly(I:C)), an inducer immunity via Toll-like...

Canine vector-borne infections gained in importance Germany due to growing tourist traffic, the increased import of dogs from abroad and changing climatic conditions. The Mediterranean region southeastern Europe are geographical areas where pathogens such as Leishmania (L.) infantum, Hepatozoon (H.) canis, Ehrlichia (E.) Anaplasma (A.) platys Dirofilaria (D.) spp. endemic. Meanwhile, Babesia (B.) A. phagocytophilum present central western Europe. objective this retrospective study was...

<b><i>Background:</i></b> The yeast <i>Malassezia pachydermatis</i> is a commensal but also the most isolated pathogenic in canine otitis externa. Essential oils (EOs) exhibit antimicrobial activity and thus could be an alternative for treatment of otitis. <b><i>Objective:</i></b> aim this study was to analyse vitro efficacy EOs against <i>M. isolates from ears. <b><i>Materials Methods:</i></b>...

Pregnancy and lactation are amongst the most challenging times of a bitch's life. Most studies focusing on endocrinological aspect pregnancy consider only small number animals. The aim this study was to evaluate progesterone (P4) concentrations in large bitches during early, mid late pregnancy. In total, 126 various breeds were recruited following thorough clinical gynecological examination estrus. Blood samples collected three (T1-T3) or from non-pregnant dogs diestrus, P4 measured via...

Objectives Three species of protozoal Hepatozoon ( H felis, canis and silvestris) are known to infect cats in Europe. The objective this study was determine the prevalence samples from living Germany that were submitted a veterinary laboratory. Methods included tested for by PCR between 2007 2020 Laboklin Travel history haematological results documented with positive test results. From 2018 onwards, partial 18S rRNA gene fragment sequenced Results Sixty-four 931 (7%) species. Sex age did not...

Mycoplasmas are an important cause of respiratory diseases in tortoises. In snakes, evidence mycoplasma infections has been found almost exclusively pythons. To better understand the occurrence these bacteria other snake species, samples submitted for routine testing pathogens were also tested by polymerase chain reaction (PCR). A total 640 (mostly oral swabs) from snakes 5 different families (Boidae n = 114, Colubridae 109, Elapidae 34, Pythonidae 301 and Viperidae 82) included study....