A5046992604- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Biotin and Related Studies
- Burkholderia infections and melioidosis
- Bacteriophages and microbial interactions
- Click Chemistry and Applications
- Respiratory viral infections research
- Supramolecular Chemistry and Complexes
- Plant Pathogenic Bacteria Studies
- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- Allergic Rhinitis and Sensitization
- Advanced Biosensing Techniques and Applications
- Food Allergy and Anaphylaxis Research
- Molecular Biology Techniques and Applications
- SARS-CoV-2 detection and testing
- Asthma and respiratory diseases
- SARS-CoV-2 and COVID-19 Research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Peptidase Inhibition and Analysis
James Cook University
2015-2025
Australian Institute of Tropical Health and Medicine
2016-2024
Townsville Hospital
2015-2018
Oregon Institute of Technology
2017
Genomics (United Kingdom)
2015
Australian National University
2003-2008
The University of Sydney
2005
Laboratoire de Chimie de Coordination
1996
Centre National de la Recherche Scientifique
1996
Information about the stability of proteins is paramount to determine their optimal storage or reaction conditions. It also essential protein in high-throughput when screening for new improved functions obtained from large mutant libraries. In drug discovery programs, monitoring ligand-induced stabilization effects can be used identify lead compounds high-throughput. These studies require expensive biophysical instrumentation and quantities purified proteins. To address these issues, we...
Cell‐free protein synthesis offers rapid access to proteins that are selectively labelled with [ 15 N]amino acids and suitable for analysis by NMR spectroscopy without chromatographic purification. A system based on an Escherichia coli cell extract was optimized regard yield minimal usage of N‐labelled amino acid, examined the presence metabolic by‐products which could interfere analysis. Yields up 1.8 mg human cyclophilin per mL reaction medium were obtained expression a synthetic gene....
During bacterial DNA replication, the DnaG primase interacts with hexameric DnaB helicase to synthesize RNA primers for extension by polymerase. In Escherichia coli, this occurs transient interaction of helicase. Here we demonstrate directly surface plasmon resonance that C-terminal domain is responsible DnaB6. Determination 2.8-angstroms crystal structure revealed an asymmetric dimer. The monomers have N-terminal helix bundle similar DnaB, followed a long connects hairpin. connecting...
Strict self-assembly of complementary dianionic and dicationic tetrahydrogen bond donors acceptors leading to infinite molecular chains in the solid state is achieved aqueous solution using both directionally controlled hydrogen bonding ion-pairing electrostatic interactions.
The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of replication. Here we show that DnaI consists a C-terminal domain (Cd) with ATPase DNA-binding activities an N-terminal (Nd) interacts helicase. A Zn2+-binding module mediates interaction C67, C70 H84 are involved in coordination Zn2+. binds ATP exhibits activity is not stimulated by ssDNA, because site on Cd masked Nd. resides when detached from Nd domain, it becomes...
The development of differential scanning fluorimetry and the high-throughput capability Thermofluor have vastly facilitated screening crystallization conditions proteins large mutant libraries in structural genomics programs, as well ligands drug discovery functional programs. These techniques are limited by their requirement for both highly purified solvatochromic dyes, fueling need more robust technologies that can be used with crude protein samples. Here, we present a new technology...
Analogs of quinolinic acid were tested for excitatory properties in evoking neurotransmitter release from striatal cholinergic interneurons and their ability to lesion these same neurons vivo (excitotoxin activity). The analogs inhibit the specific binding several ligands thought label amino receptors was also investigated. Dipicolinic (2,6-pyridine dicarboxylic acid) found be as potent efficacious (2,3-pyridine at N-methyl-D-aspartate (NMDA)-type mediating [3H]acetylcholine slices. However,...
Aggressive diagnostic testing remains an indispensable strategy for health and aged care facilities to prevent the transmission of SARS-CoV-2 in vulnerable populations. The preferred platform has shifted towards COVID-19 rapid antigen tests (RATs) identify most infectious individuals. As such, RATs are being manufactured faster than at any other time our history yet lack relevant quantitative analytics required inform on absolute analytical sensitivity enabling manufacturers maintain high...
Aminopeptidase P (APPro) is a manganese-dependent enzyme that cleaves the N-terminal amino acid from polypeptides where second residue proline. APPro shares similar fold, substrate specificity, and catalytic mechanism with methionine aminopeptidase prolidase. To investigate roles of conserved residues at active site, seven mutant forms were characterized kinetically structurally. Mutation individual metal ligands selectively abolished binding either or both Mn(II) atoms none these...
A system consisting of a protein LG coated surface for the capture mammalian antibodies (target), and an antigen fused to Tus stoichiometrically linked DNA template via Tus-Ter-lock sequence allowed ultrasensitive detection 5.5 attomol target by real-time immunoPCR in complex media.
The number of new Immuno-PCR technologies and applications is steadily growing as a result general need for more sensitive immunoassays early detection diseases. Although has been demonstrated to be superior its immunoassay counterpart, it still regarded challenging technology due various problems arising from increased power, such high background noise well substantial batch-to-batch reproducibility issues. Current efforts have intensified produce homogeneous universal protein-DNA...
Investigations into the photocrosslinking kinetics of protein Tus with various bromodeoxyuridine-substituted TerDNA variants highlight potential use this complex as a photoactivatable connector between proteins interest and specific DNA sequences.
In E. coli, DNA replication termination occurs at Ter sites and is mediated by Tus. Two clusters of five are located on each side the terminus region constrain forks in a polar manner. The polarity due to formation Tus–Ter-lock intermediate. Recently, it has been shown that DnaB helicase which unwinds fork preferentially stopped non-permissive face Tus–Ter complex without pausing efficiency sequence dependent, raising two essential questions: Does affinity Tus for different correlate with...
A simple quantitative in-gel detection system was developed for measuring production of biotin–protein conjugates using a green fluorescent streptavidin probe.
Accurate temperature control within biological and chemical reaction samples instrument calibration are essential to the diagnostic, pharmaceutical industries. This is particularly challenging for microlitre-scale reactions typically used in real-time PCR applications differential scanning fluorometry. Here, we describe development of a simple, inexpensive ratiometric dual fluorescent protein biosensor (DFPTB). A combination cycle three green monomeric red enabled quantification relative...
DnaG is the primase that lays down RNA primers on single-stranded DNA during bacterial replication. The solution structure of DnaB-helicase-binding C-terminal domain Escherichia coli was determined by NMR spectroscopy at near-neutral pH. a rare fold that, besides occurring in domains, has been described only for N-terminal DnaB. helix hairpin present domain, however, either less stable or absent DnaB, as evidenced high mobility 35 residues construct comprising 1-171. identifies previous...
The analysis of the salt dependence protein–DNA complexes provides useful information about non-specific electrostatic and sequence-specific parameters driving complex formation stability. differential scanning fluorimetry GFP-tagged protein (DSF-GTP) assay has been geared with an automatic Tm peak recognition system was applied for high-throughput (HT) determination salt-induced effects on DNA replication Tus in various Ter Ter-lock sequences. designed to generate two-dimensional heat map...