A5070988583- Neurobiology and Insect Physiology Research
- Olfactory and Sensory Function Studies
- Bacterial Genetics and Biotechnology
- Insect Utilization and Effects
- Insect Pheromone Research and Control
- Insect and Arachnid Ecology and Behavior
- Advanced biosensing and bioanalysis techniques
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Cellular transport and secretion
- Plant biochemistry and biosynthesis
- Monoclonal and Polyclonal Antibodies Research
- Insect and Pesticide Research
- Enzyme Structure and Function
- Carbon Nanotubes in Composites
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Antimicrobial Peptides and Activities
- Parasitic Infections and Diagnostics
- Electrochemical sensors and biosensors
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Potato Plant Research
Plant & Food Research
2012-2022
Australian National University
2000-2005
AgResearch
2005
The University of Sydney
1993-2005
Centro de Investigaciones Biológicas Margarita Salas
2001
Consejo Superior de Investigaciones Científicas
2001
Insect odorant receptors have been reconstituted into lipid nanodiscs and tethered to carbon nanotube field-effect transistors function as a biosensor. Here, four different insect (ORs) from Drosophila melanogaster (DmelOR10a, DmelOR22a, DmelOR35a, DmelOR71a) were expressed in Sf9 cells, purified, nanodiscs. We demonstrated that each of these ORs produce selective highly sensitive electrical response their respective positive ligands, methyl salicylate, hexanoate, trans-2-hexen-1-al,...
The Escherichia coli replication terminator protein (Tus) binds tightly and specifically to termination sites such as TerB in order halt DNA replication. To better understand the process of Tus−TerB interaction, an assay based on surface plasmon resonance was developed allow determination equilibrium dissociation constant complex (KD) association dissocation rate constants for interaction between Tus various sequences, including TerB, single-stranded DNA, two nonspecific sequences that had...
Moths recognize a wide range of volatile compounds, which they use to locate mates, food sources, and oviposition sites. These compounds are recognized by odorant receptors (OR) located within the dendritic membrane sensory neurons that extend into lymph sensilla, covering surface insect antennae. We have identified 3 genes encoding ORs from tortricid moth, Epiphyas postvittana, pest horticulture. Like Drosophila melanogaster ORs, contain 7 transmembrane helices with an intracellular...
Cold storage of tubers potato (Solanum tuberosum L.) compromises tuber quality in many cultivars by the accumulation hexose sugars a process called cold-induced sweetening.This is caused breakdown starch to sucrose, which cleaved glucose and fructose vacuolar acid invertase.During processing affected tubers, high temperatures involved baking frying cause Maillard reaction between reducing free amino acids, resulting acrylamide.cDNA clones with deduced proteins homologous known invertase...
Invasive mammalian predators threaten native ecosystems globally, and eradication efforts often fail to fully protect local flora fauna. Continuous surveillance is crucial ensure prevent reinvasion. Rapid detection of invasive species, particularly large mammals, challenging due the reliance on labour-intensive methods like traps baits. A remote sensing device capable detecting these species could be transformative for ecosystem protection. Here we present an electrochemical aptasensor that...
Graphene field effect transistor (GFET) biosensors have been used to evaluate insect odorant receptor (OR) display formats and the of presence Orco subunit on in vitro sensing using OR subunits. The were fabricated by immobilizing nanodiscs ± liposomes onto GFET devices. Two ORs from Drosophila melanogaster (DmelOR10a DmelOR22a) this study. We demonstrated that both DmelOR10a DmelOR22a showed a selective electrical response their respective positive ligands, methyl salicylate hexanoate....
Aminopeptidase P (APPro) is a manganese-dependent enzyme that cleaves the N-terminal amino acid from polypeptides where second residue proline. APPro shares similar fold, substrate specificity, and catalytic mechanism with methionine aminopeptidase prolidase. To investigate roles of conserved residues at active site, seven mutant forms were characterized kinetically structurally. Mutation individual metal ligands selectively abolished binding either or both Mn(II) atoms none these...
The Gibberellin Stimulated-Like (GSL) or Snakin peptides from higher plants are cysteine-rich, with broad spectrum activity against a range of bacterial and fungal pathogens. To detect GSL in applications such as western blot analysis enzyme-linked immunosorbent assays (ELISA), specific antibodies that recognise required. However, the intrinsic antimicrobial these is likely to prevent their expression alone yeast systems for subsequent antibody production animal hosts. overcome this issue we...
Abstract This article aims to demonstrate an electrochemically stable and reliable gold electrode‐electrolyte system develop insect odorant receptor ( Drosophila melanogaster Or35a) based bioelectronic nose. Cyclic voltammograms (CVs) electrochemical impedance spectroscopy (EIS) of bare electrodes, after modification with the self‐assembled monolayer (SAM) 6‐mercaptohexanoic acid (MHA) immobilization Or35a integrated into lipid bilayers liposomes were conducted in presence four different...
This article presents the raw and analyzed data from a set of experiments performed to study role junctions on electrostatic gating carbon nanotube (CNT) network field effect transistor (FET) aptasensors. It consists used for calculation junction bundle densities describes metallic content bundles. In addition, electrical measurement in liquid gated environment 119 different devices with four CNT summarizes their properties. The presented this are related research titled...
FtsZ is a bacterial cytoskeletal protein involved in cell division. It forms ringlike structure that attaches to the membrane complete binds and hydrolyzes GTP, assembling into polymers GTP-dependent manner. To test how orientation of monomers affects curvature filaments on surface, we performed site-directed mutagenesis E. coli insert cysteine residues at lateral locations orient planar lipid bilayers. The E93C S255C mutants were overproduced, purified, found be functionally active...