A5024522345- Opioid Use Disorder Treatment
- HIV, Drug Use, Sexual Risk
- Forensic Toxicology and Drug Analysis
- Plant Pathogens and Resistance
- Bacterial Identification and Susceptibility Testing
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Potato Plant Research
- Plant Virus Research Studies
- Poisoning and overdose treatments
- Plant Pathogenic Bacteria Studies
- Chromosomal and Genetic Variations
- Cardiac Arrest and Resuscitation
- Antibiotic Resistance in Bacteria
- Cannabis and Cannabinoid Research
- Biochemical and Structural Characterization
- Antibiotics Pharmacokinetics and Efficacy
- Pain Management and Opioid Use
- Plant Disease Resistance and Genetics
- Wildlife Ecology and Conservation
- Mycobacterium research and diagnosis
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Insect and Pesticide Research
Plant & Food Research
2012-2024
Sydney Sexual Health Centre
2016
Allan Wilson Centre
2013
Massey University
2013
University of Utah
1977
Exposure of 10 frequently isolated clinical pathogens to microwave irradiation resulted in total sterilization with 60 s. Time exposure experiments done commercially prepared test strips containing Bacillus stearothermophilus spores indicated that 5-min was adequate insure sterility small, contaminated loads.
<h3>Importance</h3> Previous unblinded clinical trials suggested that the intranasal route of naloxone hydrochloride was inferior to widely used intramuscular for reversal opioid overdose. <h3>Objective</h3> To test whether a dose administered intranasally is as effective same intramuscularly in reversing <h3>Design, Setting, and Participants</h3> A double-blind, double-dummy randomized trial conducted at Uniting Medically Supervised Injecting Centre Sydney, Australia. Clients center were...
Identification of Enterobacteriaceae by the AutoMicrobic System Biochemical Card was evaluated. Recent clinical isolates enteric gram-negative bacilli (192) and glucose nonfermenters (3) were identified System, Micro-Media Systems, Micro-ID, API, Enterotube II in comparison with conventional methods. The Systems correctly 97% organisms tested. 94, 92, 84% organisms, respectively. In addition to a high degree identification accuracy, convenient operate produced results 8 h. Operation also...
GSL1 and GSL2, Gibberellin Stimulated-Like proteins (also known as Snakin-1 Snakin-2), are cysteine-rich peptides from potato (Solanum tuberosum L.) with antimicrobial properties. Similar in other species have been implicated diverse biological processes hypothesised to play a role several aspects of plant development, responses biotic or abiotic stress through their participation hormone crosstalk, redox homeostasis. To help resolve the roles GSL2 we undertaken an depth analysis structure...
Several instruments for automated or semiautomated susceptibility testing are currently available. Three of these instruments, Autobac (General Diagnostics, Warner-Lambert Co., Morris Plains, N.J.), MS-2 (Abbott Laboratories, Dallas, Tex.), and AutoMicrobic system (AMS) (Vitek, Inc., Hazelwood, Mo.) were compared antimicrobial gram-negative bacilli. A total 207 isolates representing 29 species bacilli tested simultaneously by each instrument a standardized disk diffusion reference method....
The current phase of the North American 'opioid crisis' is characterised by illicit fentanyl use; however, presence in Australia unknown. This study aimed to monitor unintentional consumption Australia.Rapid urine drug screens (UDS) paired with surveys conducted within supervised injecting facilities (SIFs) and confirmatory laboratory testing.Sydney Melbourne, Australia.Clients who used heroin past 2 days (n = 911 tests, 2017-2021). Participants were demographically similar overall client...
Abstract New Zealand is the southernmost limit of common dolphin's (genus Delphinus ) distribution in Pacific Ocean. In this area, dolphins occur both coastal and oceanic habitats, exhibit seasonal resident occurrence, present high morphological variability. Here we investigated population structure taxonomic identity ( sp.) within waters using 14 microsatellite loci, 577 bp mt DNA control region, 1,120 cytochrome b gene across 90 individuals. We found genetic variability evidence expansion....
Abstract Introduction and Aims North America has witnessed a dramatic rise in fatal opioid overdoses due to the unwitting consumption of non‐pharmaceutical fentanyl its analogues. While some drivers this crisis—including profitability access high‐potency opioids through internet sources—also apply Australia, our knowledge, there have been no ongoing surveillance studies local populations. Therefore, pilot study aimed detect unintentional among people who inject heroin instant urine...
The Gibberellin Stimulated-Like (GSL) or Snakin peptides from higher plants are cysteine-rich, with broad spectrum activity against a range of bacterial and fungal pathogens. To detect GSL in applications such as western blot analysis enzyme-linked immunosorbent assays (ELISA), specific antibodies that recognise required. However, the intrinsic antimicrobial these is likely to prevent their expression alone yeast systems for subsequent antibody production animal hosts. overcome this issue we...
Providing information about substances injected can reduce the negative impact of illicit drug consumption and support people who inject drugs to make informed decisions. In Australia, relies largely on periodic self-report surveys. For first time, analysis residual content used injecting equipment was conducted in a supervised facility (SIF) located Sydney, Australia. The aim gain better understanding by clients through: (1) chemical analyses syringes; (2) comparison these results with...
Somatic cell selection in plants allows the recovery of spontaneous mutants from cultures. When coupled with regeneration it an effective approach for novel traits plants. This study undertook somatic potato (Solanum tuberosum L.) cultivar ‘Iwa’ using sulfonylurea herbicide, chlorsulfuron, as a positive agent. Following 5 days’ exposure suspension cultures to 20 μg/l rescue recovered rare colonies at frequency approximately one event 2.7 × 105 plated cells. Plants that were regenerated these...
An intragene involves the rearrangement of gene configurations based on switching coding sequences and regulatory regions within a species. The overexpression specific GSL (Gibberellin Stimulated-Like) genes is known to confer enhanced disease resistance. A potato intragenic expression cassette, with GSL1 placed under control light-inducible Lhca3 from potato, was designed constructed. resulting transformed using Agrobacterium-mediated transfer into cultivar Iwa investigate whether can...
A comparative evaluation was done to test the accuracy of Cathra Repliscan II agar dilution system (Diagnostic Equipment, Inc., St. Paul, Minn.), AutoMicrobic with Gram-Negative General Susceptibility-Plus Card (Vitek Systems, Hazelwood, Mo.), and Micro-Media Fox Panel micro broth (Micro-Media San Jose, Calif.) in determining MICs 12 antibiotics for 200 gram-negative bacilli. Of strains tested, isolates did not grow one three systems. The 188 remaining organisms included 158 members family...
Background: Buprenorphine and buprenorphine/naloxone (BNX) were developed to improve the safety profile of opioid substitution treatment (OST) reduce diversion injection, yet continue be injected, despite risk harm. Previous studies examining injection these substances have relied on self-reported overdose. Using data from Uniting Medically Supervised Injecting Center (MSIC) in Sydney, this study aimed assess overdose associated with use buprenorphine BNX identify factors injecting. Methods:...
Abstract Introduction The administration of illicit drugs by injection is associated with considerable harm, including an increased risk overdose. chemical analysis used syringes can enhance knowledge on injecting drug consumption beyond traditional data sources (self‐report surveys). This additional information may be useful during significant global events like the COVID‐19 pandemic. study aimed to examine a snapshot injected at Medically Supervised Injecting Centre (MSIC) in Sydney,...
Agrobacterium-mediated transformation of Nicotiana tabacum, using an intragenic T-DNA region derived entirely from the N. tabacum genome, results in equivalence micro-translocations within genomes. Intragenic gene transfer was achieved a composed DNA, including borders and acetohydroxyacid synthase conferring resistance to sulfonylurea herbicides. Genomic analysis resulting plant, with single locus inheritance herbicide resistance, identified insertion on chromosome 5. The event three DNA...