A5009443091- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- Prostate Cancer Treatment and Research
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- PARP inhibition in cancer therapy
- Circular RNAs in diseases
- Glioma Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Renal cell carcinoma treatment
- Immune Cell Function and Interaction
- Bladder and Urothelial Cancer Treatments
- Protein Degradation and Inhibitors
- DNA Repair Mechanisms
- Kidney Stones and Urolithiasis Treatments
- Cancer Research and Treatments
- Animal Genetics and Reproduction
- Pediatric Urology and Nephrology Studies
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
University of Rochester Medical Center
2016-2025
Champions Oncology (United States)
2024-2025
Center for Cancer Research
2020-2023
National Cancer Institute
2020-2023
National Institutes of Health
2021
University of Rochester
2020
National Taiwan University
2012-2014
Abstract While the androgen receptor (AR) may influence progression of clear cell renal carcinoma (ccRCC), its role to impact vasculogenic mimicry (VM) alter ccRCC and metastasis remains obscure. Here, we demonstrated that elevated AR expression was positively correlated with tumor-originated vasculogenesis in patients. Consistently, vitro research revealed promoted VM formation lines via modulating lncRNA-TANAR/TWIST1 signals. Mechanism dissection showed could increase lncRNA-TANAR (TANAR)...
Abstract Although androgen receptor (AR) can influence bladder cancer (BCa) initiation and progression, its impact on tumor immune escape remains unclear. Here, we found that targeting AR could enhance natural killer (NK) cell tumor-killing efficacy by decreasing PD-L1 expression. Both antiandrogen treatment knockdown effectively reduced membrane PD-LI expression to facilitate NK cell-mediated BCa killing downregulating circ_0001005. Mechanistically, upregulated circRNA circ_0001005 via the...
Mutations of the isocitrate dehydrogenase (IDH) gene are common genetic mutations in human malignancies. Increasing evidence indicates that IDH play critical roles malignant transformation and progression. However, therapeutic options for IDH-mutated cancers remain limited. In this study, investigation patient cohorts revealed PI3K/protein kinase B (AKT) signaling pathways were enhanced cancer cells.In we investigated expression profile cells using RNA sequencing after depletion AKT. Gene...
Cancer associated fibroblasts (CAF) play important roles in tumor growth that involves inflammation and epithelial cell differentiation. Early studies suggested estrogen receptor alpha (ERα) was expressed stromal cells normal prostates prostate cancer (PCa), but the detailed functions of ERα PCa remain to be further elucidated.Migration invasion assays demonstrated presence high levels CAF (CAF.ERα(+)) suppressed via influencing infiltration macrophages. decreased CCL5 secretion suppressing...
Abstract Crystals can trigger a wide range of kidney injuries that may link to the development stones. Infiltrating macrophages influence hyperoxaluria-induced intrarenal calcium oxalate (CaOx) crystals deposition, yet their linkage sex hormones remains unclear. Here we demonstrated suppressing androgen receptor (AR) expression in renal tubular epithelial cells increased macrophage recruitment/M2 polarization result enhancing phagocytosis CaOx crystals. Mechanism dissection suggested AR...
Fatty acid synthase (FASN) is the key enzyme for control of fatty synthesis that contributes significantly to prostate cancer (PCa) progression. It was reported androgens were able induce FASN expression in PCa, and addition anti‐androgen Casodex might suppress androgen‐induced expression. However, here we found androgen‐deprivation‐therapy (ADT) with anti‐androgens Bicalutamide (Casodex) or Enzalutamide (MDV3100) had little effect FASN‐mediated cell growth invasion during castration...
Females develop kidney stones less frequently than males do. However, it is unclear if this gender difference related to altered estrogen/estrogen receptor (ER) signaling. Here, we found that ER beta (ER β ) signals could suppress hepatic oxalate biosynthesis via transcriptional upregulation of the glyoxylate aminotransferase (AGT1) expression. Results from multiple in vitro renal cell lines also function suppressing oxalate-induced injury through increasing reactive oxygen species (ROS)...
Abstract Patient-derived models have increasingly been adopted into target validation, lead-optimization and secondary screening campaigns to move translation ahead in drug discovery. While 2-dimentional cell line vitro continue play a pivotal role early studies, new advanced 3D patient derived organoids (PDO) xenograft (PDX-O) technology made it suitable meet the rigor reproducibility of traditional metrics. At Champions Oncology, we built successful pipeline ex vivo from pre-treated,...
Abstract The human epidermal growth factor 2 receptor (HER2) is a key member of the tyrosine kinase superfamily. Alterations in HER2 have been identified various malignancies, including approximately 20% non-small-cell lung cancer (NSCLC) and about 30% breast (BRCA). These alterations encompass gene mutations, amplification, protein overexpression, all which are associated with tumorigenesis contribute to enhanced cell proliferation. Cancers often exhibit more aggressive behavior, higher...
Abstract Many anticancer drugs fail in clinical trials due to issues resulting from inadequate efficacy and/or high toxicity emphasizing the need for improving clinically relevant, preclinical models throughput drug screening. Currently, 3D have emerged as a highly promising tool initial screening that closely mimics vivo tumors through preserved morphology and molecular mechanisms. To further recapitulate models, interactions between immune tumor cells can be performed via co-culture...
Abstract Glioblastoma multiforme (GBM) is the most aggressive and fatal form of brain cancer, with a median survival time less than 15 months. Currently, GBM patients have limited treatment options poor efficacy outcomes, underscoring urgent need for innovative research methods more effective therapeutic strategies. Patient-derived xenografts (PDX) offer promising approach to evaluate side effects potency drugs in vivo. By implanting patient tumor samples into immunocompromised mice, we gain...
BackgroundWhile androgen deprivation therapy (ADT) and radiotherapy (RT) are currently used together to treat locally advanced prostate cancer (PCa), RT might have the adverse effect of increasing PCa receptor (AR) protein expression, which then increase resistance continued RT.MethodsWe multiple assays for sensitivity, RNA expression AR related DDR genes, ROS level, DNA damage/repair cell cycle apoptosis. All statistical comparisons were analyzed with t-test or one-way ANOVA.FindingsWe...
Abstract Background Radiation therapy (RT) with androgen deprivation (ADT) is an effective to suppress the locally advanced prostate cancer (PCa). However, we unexpectedly found that RT could also induce receptor splice variant 7 (ARv7) expression decrease radiosensitivity . Methods The study was designed target ARv7 Quercetin or ARv7-shRNA leads enhancing and increasing radiation sensitivity better PCa involved modulation of circNHS/miR-512-5p/XRCC5 signaling. Results Mechanism studies...
Hepatocellular carcinoma (HCC) is one of most common cancers worldwide, however, the treatment for advanced HCC remains unsatisfactory. We focused on function androgen receptor (AR) in and tried to find new strategy based antiandrogen enzalutamide (Enz). Here, we found that olaparib, a FDA-approved PARP inhibitor, could enhance cytotoxicity cells with lower BRCA1 expression, suppressing AR either Enz or AR-shRNA further increase olaparib sensitivity better suppress cell growth via...
Abstract The FDA-approved anti-androgen Enzalutamide (Enz) has been used successfully as the last line therapy to extend castration-resistant prostate cancer (CRPC) patients’ survival by an extra 4.8 months. However, CRPC patients eventually develop Enz-resistance that may involve induction of androgen receptor (AR) splicing variant ARv7. Here we found Cisplatin (Cis) or Carboplatin, currently in chemotherapy/radiation suppress tumor progression, could restore Enz sensitivity multiple...
Abstract Background Early studies indicated that ASC-J9®, an androgen receptor (AR) degradation enhancer, could suppress the prostate cancer (PCa) progression. Here we found ASC-J9® also PCa progression via AR-independent mechanism, which might involve modulating tumor suppressor ATF3 expression. Methods The lentiviral system was used to modify gene expression in C4–2, CWR22Rv1 and PC-3 cells. Western blot Immunohistochemistry were detect protein MTT Transwell assays test proliferation...